Protein Info for Rru_A1880 in Rhodospirillum rubrum S1H
Annotation: Pyruvate dehydrogenase beta subunit (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00162, pyruvate dehydrogenase E1 component subunit beta [EC: 1.2.4.1] (inferred from 100% identity to rru:Rru_A1880)Predicted SEED Role
"Pyruvate dehydrogenase E1 component beta subunit (EC 1.2.4.1); Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" (EC 1.2.4.1, EC 1.2.4.4)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.1
Use Curated BLAST to search for 1.2.4.1 or 1.2.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RT65 at UniProt or InterPro
Protein Sequence (468 amino acids)
>Rru_A1880 Pyruvate dehydrogenase beta subunit (NCBI) (Rhodospirillum rubrum S1H) MPVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQIL IEAGTQNVPVNAPIGILLEEGETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPAD TPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMRRDDKVFLLGEEVAQYQGAYK ISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAA KTLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLK AAIRDPNPVVFLENEILYGQTFEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALE AAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVTLEEGWPFAGIGAEIGMTIME NAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS