Protein Info for Rru_A1823 in Rhodospirillum rubrum S1H

Annotation: 3,4-Dihydroxy-2-butanone 4-phosphate synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00926: DHBP_synthase
  • amino acids 18 to 209 (192 residues), 292 bits, E=1.7e-91
• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 19 to 210 (192 residues), 249.3 bits, E=1.2e-78
• PF00925: GTP_cyclohydro2
  • amino acids 221 to 371 (151 residues), 131.4 bits, E=2.5e-42

Best Hits

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to rru:Rru_A1823)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

See Q2RTC2 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Rru_A1823 3,4-Dihydroxy-2-butanone 4-phosphate synthase (NCBI) (Rhodospirillum rubrum S1H)
MTDGTSPVPYRDYLASPEDIIEDARQGKMFILVDDENRENEGDLVIPAQMATAEVVNFMA
KHGRGLICLAMTRERCEQLDLPLMAQQNSSRHSTAFTISIEAKDGVTTGISAADRALTIQ
VAIDPAKGRGDIATPGHVFPLVARDGGVLVRAGHTEAAVDVARMAGLVPAGVICEVMNDD
GTMARMPDLVRFAQEHGLKVGTIADLIAYRRTKERLVRRELETDFRSVHGGDWRMMLYVS
TVSYGEHIALVKGDPRGSDPVLVRMHALNVLDDVLGDSDGNAHGQLHRAMDVIGRAGRGV
LVLIREGRPTALSERLRARIEQGPSAQRLVDYGVGAQILLDLGVTDMTLLTNTPKHIIGL
EGYGLRVAGHQPLSDKE