Protein Info for Rru_A1744 in Rhodospirillum rubrum S1H

Annotation: DNA gyrase, subunit A (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR01063: DNA gyrase, A subunit" amino acids 13 to 890 (878 residues), 1169.9 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 37 to 501 (465 residues), 527 bits, see alignment E=3.9e-162 PF03989: DNA_gyraseA_C" amino acids 534 to 580 (47 residues), 51.8 bits, see alignment 4.3e-18 amino acids 585 to 632 (48 residues), 27.5 bits, see alignment 1.7e-10 amino acids 643 to 686 (44 residues), 26.5 bits, see alignment 3.6e-10 amino acids 690 to 732 (43 residues), 36.8 bits, see alignment 2.2e-13 amino acids 793 to 839 (47 residues), 47.7 bits, see alignment 8.3e-17 amino acids 843 to 889 (47 residues), 48.5 bits, see alignment 4.6e-17

Best Hits

Swiss-Prot: 58% identical to GYRA_RICBR: DNA gyrase subunit A (gyrA) from Rickettsia bellii (strain RML369-C)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 68% identity to azl:AZL_013920)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RTK1 at UniProt or InterPro

Protein Sequence (943 amino acids)

>Rru_A1744 DNA gyrase, subunit A (NCBI) (Rhodospirillum rubrum S1H)
MSTNTPKPIDLDISPVSIEDEMKRSYLDYAMSVIVSRALPDVRDGLKPVHRRILYSMKES
GYEFNKPYRKSARIVGDVMGKYHPHGDQSIYDAMVRMVQNFAMRLPLIDGQGNFGSMDGD
RAAAMRYTEARMAKAAHSLLDDIDKETVDFQPNYDETTREPIVLPARFPNLLVNGAGGIA
VGMATNIPPHNLGEVIDACCAMIDNPAVTAEELLELVPGPDFPTGGTILGRSGVRAAHLT
GRGSVIMRGRTHFEDISGGRQAIIVTEVPYQVNKASMVEKIAELVREKKLEGISDIRDES
DRRGVRVVIEVKRDAMAEVVLNQLFRYTPLQTSFGVNMLALNGGKPELLALRPIIEAFIT
FREEVIRRRTIFELGKARERAHTLVGLAIAVANLDPVIKLIREAPDPQAAREGLMARDWP
AMDVEPLIKLIDEPGRMVIDGVYRLSEIQARAILDLRLHRLTGLERDKLHGELIEIGQQI
EEYLHILRSRARLFEIMRAELVEMKEAYATPRRTTLEENEFEHDIEDLIQREEMVVTVTN
TGYIKRVPLTTYRAQRRGGRGRAGMSMRDEDFVTTIFVVNTHAPLLFFSSDGMVYKMKVY
RLPLGTPQARGKAMVNIMPLNEGERITTVMPLPEDEGAWDQLDVMFATETGNVRRNRLSD
FTNVMSNGKIAMKLDEGDHLIGVATCTDEQDVLLTTNLGKAIRFAVGDVRVFAGRTSTGV
RGIRLAEDDRVNRLSILGHVEASAETRAAYLKQASALRRASGETEGEDDSTLDAEPGVLS
AEEFEDLRQREEFLLTVTSRGFGKRTSAYEYRVTGRGGQGIANIEMSERNGTVVAAFPIA
NEDQVMMITDGGQLIRMPVNDVRIAGRKTQGVTLFRLSDDGEHVVSVARLPEMNGNGDDG
EGDDETPDESLAEDANGGRDESGEASGLPPAGQSPERNGDDAD