Protein Info for Rru_A1674 in Rhodospirillum rubrum S1H

Annotation: bifunctional enzyme, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, 4-diphosphocytidyl-2C- methyl-D-erythritol synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01128: IspD" amino acids 4 to 220 (217 residues), 220.2 bits, see alignment E=4.7e-69 TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase" amino acids 6 to 220 (215 residues), 228.2 bits, see alignment E=8.1e-72 PF12804: NTP_transf_3" amino acids 6 to 133 (128 residues), 45.2 bits, see alignment E=1.7e-15 PF02542: YgbB" amino acids 228 to 380 (153 residues), 206.1 bits, see alignment E=5.7e-65 TIGR00151: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase" amino acids 228 to 380 (153 residues), 201.7 bits, see alignment E=6.8e-64

Best Hits

Swiss-Prot: 100% identical to ISPDF_RHORT: Bifunctional enzyme IspD/IspF (ispDF) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K12506, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [EC: 2.7.7.60 4.6.1.12] (inferred from 100% identity to rru:Rru_A1674)

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis or Teichoic and lipoteichoic acids biosynthesis (EC 2.7.7.60, EC 4.6.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60 or 4.6.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RTS1 at UniProt or InterPro

Protein Sequence (384 amino acids)

>Rru_A1674 bifunctional enzyme, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, 4-diphosphocytidyl-2C- methyl-D-erythritol synthase (NCBI) (Rhodospirillum rubrum S1H)
MAKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRD
LYGAAAHGLATLLDAVEGGAERQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVI
AALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQTPQGFRYDAILAAHRAQAGT
MLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGP
GDFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSA
IFLRHAAALITALGGRILNVDITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTT
EELGFTGRGEGIAAQATALITLPF