Protein Info for Rru_A1531 in Rhodospirillum rubrum S1H

Annotation: 5-methyltetrahydrofolate--homocysteine methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 8 to 1182 (1175 residues), 1252 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 13 to 302 (290 residues), 272.1 bits, E=2e-84
• PF00809: Pterin_bind
  • amino acids 344 to 577 (234 residues), 137.5 bits, E=1.6e-43
• PF02607: B12-binding_2
  • amino acids 633 to 706 (74 residues), 77.9 bits, 1.4e-25
• PF02310: B12-binding
  • amino acids 720 to 827 (108 residues), 65.7 bits, E=8.7e-22
• PF02965: Met_synt_B12
  • amino acids 936 to 1182 (247 residues), 154.5 bits, E=9.8e-49

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 100% identity to rru:Rru_A1531)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• folate transformations II (plants) (7/11 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (8/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q2RU64 at UniProt or InterPro

Protein Sequence (1182 amino acids)

>Rru_A1531 5-methyltetrahydrofolate--homocysteine methyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MSRFLDHLRNRVLLCDGGMGSLVQAMDLSVDGDFMGRENCTEVLNLSRPDVVRDIHTRYF
AAGADCVETNTFGGSTLTLAEFDLADRTREINRRAAEIAREAAESFADGRDRFVLGSIGP
GTKLPSLGHIDYDTLKDAITVQCEGLIEGGADAILVETCQDPLQFKAAINAAKAARLALG
SDTAILLQVTVETTGTLLVGADIAAAATVAHALGVDSLGLNCATGPQEMSEHVRWLKENW
PGLISLQPNAGLPELLDGKTHYPLTPAELADWHGRFIREDGVNLIGGCCGTTPDHIAAVN
AMLETLGQGRRPAPVKRSVHWVPSVASLYGAVPMRQENAFLSIGERCNANGSKKFRTFQD
NEDWDAIVGMAREQVKEGSHTLDVCTAFVGRKEGADMTEVVSRLRGGVNAPLVIDSTETP
VLTQALKLYGGKAIINSINFEDGEESAAQRLALAREFGAAVIALTIDEEGMAKDAEAKLR
IAHRLYDFAVTQHGLPASDLMFDPLTFTICTGNTDDRRLGLETLDAIRRIRTELPECQII
LGLSNISFGLKPVARHVLNSVFLDKAVEAGMTGAIVHISKILPLHKIPADEVKAAEDLIF
DRRAPGYDPLHAFIALFEDRSETKVAVKRAETVEERLKQRIVDGDRLGLDADLTEAMGKY
PPLEIINSLLLDGMKVVGELFGAGKMQLPFVLQSAETMKAAVAFLEPHMEKIEGQEKGII
VLATVKGDVHDIGKNLVDIILSNNGYKVINIGIKQPIAAILASARENRADAIGMSGLLVK
STVIMKDNLEEMAREGWNTPVLLGGAALTRAFVEEDCVSAYAGSGRVAYARDAFDGLDLM
AKVAEGRFDTHLSAVQQKRAGRPSRRPKAPPLWPANDPSSKIAPRTLDDQDTAIHKISER
PVDAEEILLRRAELARDHVVPTPPFWGAKVLESISLKSLVPYINEATLFQFQWGFRKGGR
TREEWKDWAASEIRPIMFDMLKTCAQEEILKPRAVYGFWKAASQGDAIVLFAEDGTTEVG
RFDLPRQTIEGGICLADFVRDIDSGERDVIALQAVTVGARASDVCRDWFAANRYKDYLYL
HGISVEVAEAMAEYVHKRIRAELGFAAEEARDIERMIKQEYRGSRYSFGYPACPNLGDQR
QLLDLLGAERIELDLSDEDELVPEQSTSALVLLHPQAKYFRI