Protein Info for Rru_A1500 in Rhodospirillum rubrum S1H

Annotation: Signal Transduction Histidine Kinase (STHK) with CheB and CheR activity (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1483 PF01339: CheB_methylest" amino acids 42 to 217 (176 residues), 184.6 bits, see alignment E=6.8e-58 PF03705: CheR_N" amino acids 249 to 297 (49 residues), 29.4 bits, see alignment (E = 2.6e-10) PF01739: CheR" amino acids 311 to 500 (190 residues), 164.5 bits, see alignment E=1e-51 PF13596: PAS_10" amino acids 758 to 863 (106 residues), 88 bits, see alignment 2.9e-28 PF08448: PAS_4" amino acids 764 to 866 (103 residues), 35.6 bits, see alignment 4.7e-12 PF00512: HisKA" amino acids 885 to 948 (64 residues), 29.8 bits, see alignment (E = 2.6e-10) PF02518: HATPase_c" amino acids 995 to 1101 (107 residues), 81.1 bits, see alignment 4.2e-26 PF00072: Response_reg" amino acids 1134 to 1246 (113 residues), 50 bits, see alignment 1.6e-16 amino acids 1277 to 1386 (110 residues), 81.6 bits, see alignment 2.3e-26 PF08281: Sigma70_r4_2" amino acids 1411 to 1459 (49 residues), 34.7 bits, see alignment (E = 5.5e-12) PF00196: GerE" amino acids 1416 to 1471 (56 residues), 67.6 bits, see alignment (E = 2.9e-22)

Best Hits

KEGG orthology group: K13924, two-component system, chemotaxis family, CheB/CheR fusion protein [EC: 2.1.1.80 3.1.1.61] (inferred from 100% identity to rru:Rru_A1500)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80, 3.1.1.61

Use Curated BLAST to search for 2.1.1.80 or 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RU94 at UniProt or InterPro

Protein Sequence (1483 amino acids)

>Rru_A1500 Signal Transduction Histidine Kinase (STHK) with CheB and CheR activity (NCBI) (Rhodospirillum rubrum S1H)
MPRRSFPWAGAVRSRAEEGVSPTDGASEGKGGSGEADRFRVVGIGASAGGLEACKALLAA
LPDRPGIALILVQHLDPTHESMMVALLALQTTMTVCQAAEGMIVEPDHLYVIPPGAYLSV
AGGTLHLSPPQARHGARLPFDFLLHSLAADYTQRAICVVLSGTGADGSLGLMALKERGGL
VIAQDPEEAGYDGMPRSAIATGQVDLVLSLAKIAETLVGEDLPPGKPALPEEIPSSKGKG
KGKGWVYAVIDLLRARTPYDFTHYKPGTLERRTERRMTLASIEVGDSARYLALLQEDATE
RDLLAKDLLINVTGFFRDPKVFDVLAEKTIPQMVATHPLDQPLRIWVAGCSTGEETYSLA
ILFREAIVSAGRAVKVQVFASDVDPDAVLSAREGRYPSTIEADVSAERLARFFTRDGDDY
RILPDLRSMVVFTVQDLLADPPFSRLDMVSCRNLLIYLRPEAQAKAIGLFHFALREGGIL
LLGNSETAGNVEGRFEVLSKSARLYRRIGRGGRVDLRLPVVGGDGSGAQRRPLFEPARSR
QATLADLCRRLVVEAYAPAAVLITLKYDCLYFLGAMDSYLRVAAGHPASDLLSLAREEVR
TKLRSAIEMACETKTRTLVTGGRLTHGGTVRGFSIAVHPVSHDGEDLLLVCFLDEVPSER
RADTPTSAEHAPRVAELEQELEITRSELRGAIRNLEISSEEQKAINEEALSVSEEYQATN
EELLASKEELQSLNEELTALNGQLHETLERQRTTSNDLQNVLYSTDVATLFLDTALNIRF
FTPATKLLFRVIPGDIGRPLADLSPLVADDALLGDAAKVLKTMTPIEREIEARSGSWYLR
RILPYRTQDNGVEGVVITFADITERRWAADAREVAKRQAQIANMAKSRFLAVASHDLRQP
LQTLTLLQGLLAKKVEGVQSRVLLARMDETLGAMSSMLNVLLDINQIEVGTVEAERVVFP
INDLFDRVREELAYQARAKKLDWSVVSCGLSVTSDPRLLEQMIRNLLANALKYTKQGKVL
LGCRRRSGSLSIEVWDSGIGIPPGELEAIFDEFHQVETPAQGQGRGLGLGLSIVRRLGDL
LGHPIGVRSRLGKGSVFTINVAIAPAAAEKPSLPARRGAAEVKVDADGQRTGEILIIEDD
PDVRDLLDLLLKGEGHRTVAVAEGGQAVALAEGGSIRPDLILADYNLPNGVNGLGVSATL
RDVLDRPIPVVILTGDISTRTLHDIARHRCVKLNKPVKPGELTQTIARLLPPPVGPAAAR
LPAPPPSPGPGGPLPLIYLVDDDASLRDSLRALLEDDGLKVEDYESSEAFLAAYRPGGGE
ACLLIDAYLPGMSGLDLLERLKKENRLLPAIVITGYSEVAMAVQAMKAGASDFIEKPVGR
TELLISIDRALEQHREHGNSGKMRLWRDAAKARLASLSARQREIMEMVLAGQPSKNIAAD
LGISQRTVESHRALIMKKLGVKSLPALVRLAQAASDGEVGEEG