Protein Info for Rru_A1392 in Rhodospirillum rubrum S1H

Annotation: Nitrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 TIGR02931: Fe-only nitrogenase, beta subunit" amino acids 2 to 462 (461 residues), 969 bits, see alignment E=1.7e-296 PF00148: Oxidored_nitro" amino acids 20 to 445 (426 residues), 367.3 bits, see alignment E=4.6e-114

Best Hits

Swiss-Prot: 80% identical to ANFK_AZOVI: Nitrogenase iron-iron protein beta chain (anfK) from Azotobacter vinelandii

KEGG orthology group: K02591, nitrogenase molybdenum-iron protein beta chain [EC: 1.18.6.1] (inferred from 100% identity to rru:Rru_A1392)

MetaCyc: 80% identical to dinitrogenase 3 subunit beta (Azotobacter vinelandii)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (iron-iron) beta chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RUK2 at UniProt or InterPro

Protein Sequence (462 amino acids)

>Rru_A1392 Nitrogenase (NCBI) (Rhodospirillum rubrum S1H)
MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES
FEIASSSVHEDGAVFGALDRVEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKL
EEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVKAFAKKGKPSLKINLITGWV
NPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA
LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAI
DALTDLVHMFLADKKVAIYGNPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKAL
QERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR
AGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW