Protein Info for Rru_A1322 in Rhodospirillum rubrum S1H

Annotation: alpha subunit of malonate decarboxylase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 PF16957: Mal_decarbox_Al" amino acids 9 to 557 (549 residues), 927.2 bits, see alignment E=1.3e-283 TIGR01110: malonate decarboxylase, alpha subunit" amino acids 10 to 557 (548 residues), 957.2 bits, see alignment E=1.2e-292

Best Hits

Swiss-Prot: 74% identical to MADA_MALRU: Acetyl-S-ACP:malonate ACP transferase (madA) from Malonomonas rubra

KEGG orthology group: K13929, malonate decarboxylase alpha subunit [EC: 2.3.1.187] (inferred from 100% identity to rru:Rru_A1322)

MetaCyc: 74% identical to acetyl-[acyl-carrier protein]:malonate [acyl-carrier protein] transferase (Malonomonas rubra)
RXN-9774 [EC: 7.2.4.4]; Acetyl-S-ACP:malonate ACP transferase. [EC: 7.2.4.4, 2.3.1.187]

Predicted SEED Role

"Malonate decarboxylase alpha subunit" in subsystem Malonate decarboxylase

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.187 or 7.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RUS2 at UniProt or InterPro

Protein Sequence (557 amino acids)

>Rru_A1322 alpha subunit of malonate decarboxylase (NCBI) (Rhodospirillum rubrum S1H)
MSKTGTDKKWDHRSVDTAERLAAAARFIGPGKEVKAADTVALLEAVIRAGDRVNIEGNNQ
KQADFLAECLNKVDKSKIHDLHMVQSAVPLPVHLDLFENGIAKKLDFAFGGPQAGRVAKF
IKEGKLELGAIHTYLELFGRYFLDLTPRVSLICAYKADRQGNLYTGFNTEDTPTIVEATK
FRQGIVIAQVNEIVDTLPRVDIPADWVDVVIESPQPFFLEPLFTRDPALVTDSQILLGML
CLKGIYAEYGVQRINHGIGFLTSAIELMLPTYGEELGLKGKVCTHMILNPHPTMIPAIES
GWVDTIHCFGGELGMEEYVAARPDIFFVGPDGTLRSNRAFAQTAGHYALDMFIGGTLQID
KFGNSSTATANRVAGFGGAPNMGCDSKGRRHSTASWLKCGAEQPTLDRQLGTMPRGKRLV
VQGVETFGEGRAPVFVDKLDAWELAENAGLDLPPVMIYGDDLTHVVTEEGIAYLNRCPDI
DSRMAAIRAVAGYTELGLQAKPSETRALRDAGIVKTPEDLGVDRSRANRSMLAARNIRDL
VSWSGGLYNPPARFRNW