Protein Info for Rru_A1027 in Rhodospirillum rubrum S1H

Annotation: (Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 transmembrane" amino acids 162 to 181 (20 residues), see Phobius details amino acids 187 to 205 (19 residues), see Phobius details amino acids 213 to 231 (19 residues), see Phobius details amino acids 237 to 255 (19 residues), see Phobius details amino acids 387 to 407 (21 residues), see Phobius details amino acids 416 to 442 (27 residues), see Phobius details amino acids 725 to 746 (22 residues), see Phobius details amino acids 752 to 773 (22 residues), see Phobius details PF00403: HMA" amino acids 24 to 82 (59 residues), 49.7 bits, see alignment 5.8e-17 TIGR01525: heavy metal translocating P-type ATPase" amino acids 216 to 772 (557 residues), 555.9 bits, see alignment E=1.9e-170 TIGR01494: HAD ATPase, P-type, family IC" amino acids 239 to 488 (250 residues), 131.3 bits, see alignment E=5.3e-42 amino acids 590 to 747 (158 residues), 119.7 bits, see alignment E=1.8e-38 PF00122: E1-E2_ATPase" amino acids 267 to 448 (182 residues), 179 bits, see alignment E=1e-56 PF00702: Hydrolase" amino acids 465 to 684 (220 residues), 105.2 bits, see alignment E=8.9e-34

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 99% identity to rru:Rru_A1027)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVL7 at UniProt or InterPro

Protein Sequence (777 amino acids)

>Rru_A1027 (Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase (NCBI) (Rhodospirillum rubrum S1H)
MRQDKHASTPLRPGEAEGFRRTRYRVAGMDCGSCAAKIERALMPLPGVAQVGVSVTAGTL
TVDHGPKVGRAQIVRIVDALGFTVLEDGPNPTTSPATVVSSCGCSGSCADGVPRAASHDH
DHDHDHDHDHDHDHDHEHGHDHGASPVGDVEGRRWWRSGRGMLTLGCGAALAVAYGLGLV
FPPLADGLFIAAMLIGLLPIARRAFTSARYGSPFSIETLMTVAALGAVALGAVEEAAAVV
FLFLVGEALEGLAAGRARAGIRALSALVPKTAQREGADGGVREVLADRLAIGDVIVVRPG
DRVPADGLVLSGNGEVDEAPVTGESLPKAKAAGDGLFAGTINGIGLLRVRVTAAAADNTI
ARVVRLVEEATESKAPIARFIDRFARLYTPAVIAAALAVAVLPPMVLGGAWETWIYRALA
LLLIGCPCALVISTPAAIAAGLSAGARRGLLIKGGAVLEILGKVTTVAFDKTGTLTRGEP
VVTDLQARALSAGEALALAAGLAEGSSHPVSRAILARARLDAILPRRIDDAGTRAGEGLD
GTVDGEALFLGSPKAARRRMGGFEDDVDGAILALEQAGKTVSLLVLGEGATARVGAVFGL
RDEPRPDAGEGVAALKAAGVEAMMLTGDTPHAARALGQGLDIAVHAGLMPEDKLRLVRER
QNRGALVAVVGDGINDAPALAAADVGIAMGGGTDVALETADAAILRSRVGDVAAMIDLSK
RTLAIIHQNVAIALGLKALFLVTTVAGLTGLWPAILADTGATVLVTANALRLLRRRR