Protein Info for Rru_A1011 in Rhodospirillum rubrum S1H

Annotation: Nitrogenase molybdenum-iron protein alpha chain (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 16 to 481 (466 residues), 863.6 bits, see alignment E=3.2e-264 TIGR01862: nitrogenase component I, alpha chain" amino acids 31 to 475 (445 residues), 623.9 bits, see alignment E=1.3e-191 PF00148: Oxidored_nitro" amino acids 65 to 469 (405 residues), 355.8 bits, see alignment E=1.4e-110

Best Hits

Swiss-Prot: 78% identical to NIFD_SINFN: Nitrogenase molybdenum-iron protein alpha chain (nifD1) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 100% identity to rru:Rru_A1011)

MetaCyc: 73% identical to [FeMo]-nitrogenase complex dinitrogenase component alpha subunit (Azotobacter vinelandii)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVN3 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Rru_A1011 Nitrogenase molybdenum-iron protein alpha chain (NCBI) (Rhodospirillum rubrum S1H)
MSLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVM
TIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSD
FQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIGLIGDDIEAVARKKAKDVGLP
IIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWAS
RILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYN
FFGPSQIEKSLRAIAANFDETIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGL
RPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGTLIYDDLTGYELERFIEKLRP
DLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAP
WTKAAAE