Protein Info for Rru_A0955 in Rhodospirillum rubrum S1H

Annotation: UDP-N-acetylmuramyl-tripeptide synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 29 to 487 (459 residues), 443.6 bits, see alignment E=4.7e-137 PF01225: Mur_ligase" amino acids 30 to 98 (69 residues), 36.2 bits, see alignment E=9.7e-13 PF08245: Mur_ligase_M" amino acids 115 to 318 (204 residues), 176.1 bits, see alignment E=1.4e-55 PF02875: Mur_ligase_C" amino acids 338 to 424 (87 residues), 81.6 bits, see alignment E=6.3e-27

Best Hits

Swiss-Prot: 49% identical to MURE_AGRFC: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to rru:Rru_A0955)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVT9 at UniProt or InterPro

Protein Sequence (493 amino acids)

>Rru_A0955 UDP-N-acetylmuramyl-tripeptide synthetase (NCBI) (Rhodospirillum rubrum S1H)
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFI
PAALAAGASAVLAPEGTALPPDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNG
KTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTTPDPVSLHKMLAEMTEAGVDA
LAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLL
PLVGDFQAMNVLCALGLAIATGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAH
TPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGRAVAEGADVAILTDDNPRSED
PAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
ADHARAAVGEADA