Protein Info for Rru_A0903 in Rhodospirillum rubrum S1H
Annotation: Pyruvate formate-lyase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 100% identity to rru:Rru_A0903)Predicted SEED Role
"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)
MetaCyc Pathways
- L-threonine degradation I (6/6 steps found)
- pyruvate fermentation to acetate IV (3/3 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
- reductive monocarboxylic acid cycle (2/2 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
- superpathway of L-threonine metabolism (13/18 steps found)
- pyruvate fermentation to ethanol I (2/3 steps found)
- superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
- mixed acid fermentation (11/16 steps found)
- superpathway of N-acetylneuraminate degradation (13/22 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.54
Use Curated BLAST to search for 2.3.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RVZ1 at UniProt or InterPro
Protein Sequence (846 amino acids)
>Rru_A0903 Pyruvate formate-lyase (NCBI) (Rhodospirillum rubrum S1H) MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFN NLPVTIRNDELIVGAITKNPRSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELH DAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYGGVGHVCVDYGKVLKIGFRGI ITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADK SIDKGFAQELVDCIWIKLNDVNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISY MCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGLGVPAMYNDEVIVPALQNRGV TLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGG AIYNFTGPQAFGVADTGDSVYAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGD FTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPAAGGSSTKYDAIRRLLDASPA FGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFAS LVRSYFDHKGMHVQFNVIDRQTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIIS RTEQTL