Protein Info for Rru_A0858 in Rhodospirillum rubrum S1H

Annotation: Drug resistance transporter EmrB/QacA subfamily (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 54 to 76 (23 residues), see Phobius details amino acids 83 to 102 (20 residues), see Phobius details amino acids 108 to 130 (23 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 169 to 191 (23 residues), see Phobius details amino acids 201 to 222 (22 residues), see Phobius details amino acids 234 to 254 (21 residues), see Phobius details amino acids 275 to 297 (23 residues), see Phobius details amino acids 303 to 325 (23 residues), see Phobius details amino acids 337 to 354 (18 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details amino acids 402 to 421 (20 residues), see Phobius details amino acids 481 to 499 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 16 to 497 (482 residues), 492.5 bits, see alignment E=6.7e-152 PF07690: MFS_1" amino acids 21 to 414 (394 residues), 172.5 bits, see alignment E=6.1e-55

Best Hits

Swiss-Prot: 53% identical to EMRB_ECOLI: Multidrug export protein EmrB (emrB) from Escherichia coli (strain K12)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to rru:Rru_A0858)

MetaCyc: 53% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RW36 at UniProt or InterPro

Protein Sequence (515 amino acids)

>Rru_A0858 Drug resistance transporter EmrB/QacA subfamily (NCBI) (Rhodospirillum rubrum S1H)
MPPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSF
GVSNAIAVPLTGWLVGRIGQLRLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGP
MVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLGGWLTDNVSWPWIFYINGPIG
LIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALA
IIAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSV
MGYTATWAGLVTAPIGFFSLLLSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDV
ALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAASGLYNFMRIMAGSFAASVWTT
LWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITD
LFWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR