Protein Info for Rru_A0773 in Rhodospirillum rubrum S1H

Annotation: Nitrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00148: Oxidored_nitro
  • amino acids 53 to 453 (401 residues), 306.6 bits, E=1.3e-95

Best Hits

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 100% identity to rru:Rru_A0773)

MetaCyc: 41% identical to methylthio-alkane reductase catalytic subunit alpha (Rhodospirillum rubrum)

Predicted SEED Role

"Nitrogenase vanadium-cofactor synthesis protein VnfE" in subsystem Nitrogen fixation

MetaCyc Pathways

• nitrogen fixation I (ferredoxin) (1/1 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

See Q2RWB8 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Rru_A0773 Nitrogenase (NCBI) (Rhodospirillum rubrum S1H)
MTKIEKPLQPVEIRERRLGSITGYSGTAGDLVARSRDGSLANRERSFQQASMCAGLCATL
NLTLIEDAVVINHAPVGCAGDFAQLNRCRRRGQGKRAMEVTNARLLSTALTEHDTIFGGA
QKLAETIDQAVARYHPRAIFVTASCASGIVGEDIDSVVTEAEERHAIPVVAIDCVGFRTQ
VWATGFDMAYDALLRKIVKPAVTKRPEVVNIVCFSGKKAYFSELLEPLGLVANPIVQFLT
VAEIETLSEAGATAHMCPTLGTWLGAGLERRFGVPEVKAPPPYGLAGTDHWLRALAALTG
RSDRVEAYIAAERAAIAPDLADLRAQLAGRTAFVAAGSAQGHSFMTVLRDLGIDVIGGCA
LHHDPVLDHGHQEEDTLAHATRDKAEVPYGVCNKQSFELINLVGKLKPDILLIRHPGLVV
WGAKLGIPTLYVEDEHAGIGYRGLIRYGRKIADWIANPVLERTLARRSPLPFTPWWLDQS
PYQFLES