Protein Info for Rru_A0695 in Rhodospirillum rubrum S1H

Annotation: 2-isopropylmalate synthase/homocitrate synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 533 TIGR00977: citramalate synthase" amino acids 6 to 528 (523 residues), 674.6 bits, see alignment E=3.9e-207 PF00682: HMGL-like" amino acids 6 to 271 (266 residues), 148.4 bits, see alignment E=3e-47 PF08502: LeuA_dimer" amino acids 378 to 521 (144 residues), 79 bits, see alignment E=3.2e-26

Best Hits

Swiss-Prot: 51% identical to CIMA_AQUAE: (R)-citramalate synthase (cimA) from Aquifex aeolicus (strain VF5)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to rru:Rru_A0695)

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RWJ6 at UniProt or InterPro

Protein Sequence (533 amino acids)

>Rru_A0695 2-isopropylmalate synthase/homocitrate synthase (NCBI) (Rhodospirillum rubrum S1H)
MSDKRVYLYDSTLRDGAQTQGVDFSATDKAAIARELDALGIDYVEGGWPGANPTDDALFA
NPPVLRRARLSAFGMTRRSGRSAENDPSLQALFCPGVRVVTMVGKTWDFQCEVAIGVPLD
ENRKMIADSIAHAAARVEEAVFDAEHFFDGYKSNPDYALSCVRAAYEAGARWVVLCDTNG
GTLPHQIEAIVGAVAAVIPGDHLGIHCHNDTENAVANSLAAVRAGARQVQGTLNGLGERC
GNANLVSIIPNLMLKMGFETGVSEHDLRHLTHVSRMLDERLNRAPNRNAAFVGDSAFAHK
GGLHVSAVERDPRCYEHIAPETVGNARQILVSNQSGRSNVIARFREIGLEIDPGEPKVND
LLDAVKRRESEGYAYDGAEASFELLARRALEGVPEYFRLNSFRVIDERRWNARGQLITLS
EATIKAVVQGRDYMTVAEGNGPINALDAALRKVLLPVYPQLADLRLVDYKVRILTPDDAT
AAITRVMIESADSKGGRWNTVGVSGNIIDASYNALHDSLTYKLFKGEQASRAA