Protein Info for Rru_A0671 in Rhodospirillum rubrum S1H

Annotation: cobalamin synthesis CobW protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR02475: cobalamin biosynthesis protein CobW" amino acids 10 to 346 (337 residues), 511.6 bits, see alignment E=4.1e-158 PF02492: cobW" amino acids 13 to 214 (202 residues), 182.7 bits, see alignment E=5.5e-58 PF07683: CobW_C" amino acids 260 to 347 (88 residues), 62 bits, see alignment E=4e-21

Best Hits

Swiss-Prot: 56% identical to COBW_SINSX: Protein CobW (cobW) from Sinorhizobium sp.

KEGG orthology group: K02234, cobalamin biosynthesis protein CobW (inferred from 100% identity to rru:Rru_A0671)

Predicted SEED Role

"CobW GTPase involved in cobalt insertion for B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RWM0 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Rru_A0671 cobalamin synthesis CobW protein (NCBI) (Rhodospirillum rubrum S1H)
MSVSARHAPRRIPATVITGFLGAGKTTLVRHLMEHAGGRRIALIINEFGDVGMDREILAA
CGVAGCAEDDIVELANGCICCTVADEFLPTMEALLARPQPPEHIIIETSGLALPKPLVRA
FDWPDVRARVTVDGVVAVVDARSVHDGRFTEDPAGLAARRAADPALDHDNPLEEVFEDQL
LCADMVVLNKVDLLSEAETAALIAELGGRLRPGTRLVPVVEGRVDPAVALGLMAGVEDDL
EGRPSHHDTATDHDHDDFHSFVVGLGAVADPAEARQRLGAVIADHDILRIKGFLAVPGKP
MRHLIQAVGPRVSGYYDRPWRDDEARESRLVVIGLRGLDEAAIRGALVGL