Protein Info for Rru_A0570 in Rhodospirillum rubrum S1H

Annotation: Glutamate--cysteine ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 TIGR01436: glutamate--cysteine ligase" amino acids 13 to 456 (444 residues), 690.4 bits, see alignment E=3.9e-212 PF04107: GCS2" amino acids 54 to 360 (307 residues), 314.5 bits, see alignment E=3.7e-98

Best Hits

Swiss-Prot: 60% identical to GSH1_TOBAC: Glutamate--cysteine ligase, chloroplastic (GSH1) from Nicotiana tabacum

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to rru:Rru_A0570)

MetaCyc: 61% identical to glutamate-cysteine ligase (Arabidopsis thaliana col)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RWX1 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Rru_A0570 Glutamate--cysteine ligase (NCBI) (Rhodospirillum rubrum S1H)
MSSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGV
RALLEGLTRFGWSPVYEGETVIALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTH
HRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLMSEYMPKVGSLGLDMMLRTCT
VQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPART
GILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTI
ADWTDHMTVAFPEVRLKRYLEMRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDW
TAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAGLAARGRLNSKGRDETGFLDP
LWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY