Protein Info for Rru_A0466 in Rhodospirillum rubrum S1H

Annotation: tRNA isopentenyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 TIGR00174: tRNA dimethylallyltransferase" amino acids 13 to 291 (279 residues), 210 bits, see alignment E=2.3e-66 PF01745: IPT" amino acids 14 to 61 (48 residues), 26 bits, see alignment 5.9e-10 PF01715: IPPT" amino acids 46 to 290 (245 residues), 228.1 bits, see alignment E=1.4e-71

Best Hits

Swiss-Prot: 100% identical to MIAA_RHORT: tRNA dimethylallyltransferase (miaA) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 100% identity to rru:Rru_A0466)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RX74 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Rru_A0466 tRNA isopentenyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSV
EDEAAAPHALYGVLDGAEVCSVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGL
ASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAERLRPSDAQRIARAYEVVRGTG
RSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSES
LVTQLSESQLTDIDNFLYAFLLTGAS