Protein Info for Rru_A0273 in Rhodospirillum rubrum S1H

Annotation: Acetoacetyl-CoA reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR01829: acetoacetyl-CoA reductase" amino acids 5 to 241 (237 residues), 350.1 bits, see alignment E=3.4e-109 PF00106: adh_short" amino acids 5 to 190 (186 residues), 196.8 bits, see alignment E=4e-62 PF08659: KR" amino acids 7 to 181 (175 residues), 66.9 bits, see alignment E=3.3e-22 PF13561: adh_short_C2" amino acids 14 to 240 (227 residues), 198.9 bits, see alignment E=1.5e-62

Best Hits

Swiss-Prot: 65% identical to PHAB_RHIME: Acetoacetyl-CoA reductase (phaB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 100% identity to rru:Rru_A0273)

MetaCyc: 64% identical to (R)-3-hydroxybutyryl-CoA dehydrogenase (Cereibacter sphaeroides)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXR7 at UniProt or InterPro

Protein Sequence (242 amino acids)

>Rru_A0273 Acetoacetyl-CoA reductase (NCBI) (Rhodospirillum rubrum S1H)
MTKGRVALVTGGTRGIGAAISLALRDAGYRVAANYYGNDAAAERFTEENKIPAFKFDVAE
YAAVQEGVKQITAELGPIEVLVNNAGITRDGTMHRMTPEQWEEVIHTNLSSCFNLARGVI
DSMRDRGFGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKALAQEAAAKGITVNAIAPG
YVDTDMVRAVPYHVLEKIIAKIPMGRLGRAEDIARGVLFLVADDADYITGATLSINGGQH
MY