Protein Info for Rru_A0052 in Rhodospirillum rubrum S1H

Annotation: Biotin carboxylase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 109 (109 residues), 144.2 bits, E=1.1e-45
• PF08443: RimK
  • amino acids 115 to 313 (199 residues), 26.4 bits, E=2.4e-09
• PF02786: CPSase_L_D2
  • amino acids 115 to 321 (207 residues), 263.2 bits, E=7.8e-82
• PF02222: ATP-grasp
  • amino acids 125 to 277 (153 residues), 29.4 bits, E=3.1e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 134 to 290 (157 residues), 36.9 bits, E=1.4e-12
• PF02785: Biotin_carb_C
  • amino acids 335 to 443 (109 residues), 127.7 bits, E=1e-40
• PF18140: PCC_BT
  • amino acids 463 to 586 (124 residues), 128.8 bits, E=6.8e-41
• PF00364: Biotin_lipoyl
  • amino acids 597 to 659 (63 residues), 59 bits, E=1.7e-19
• PF13533: Biotin_lipoyl_2
  • amino acids 597 to 629 (33 residues), 23.5 bits, see alignment (E = 1.9e-08)

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 100% identity to rru:Rru_A0052)

MetaCyc: 60% identical to propionyl-CoA carboxylase alpha subunit (Cereibacter sphaeroides)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module (EC 6.4.1.3)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• methylaspartate cycle (15/19 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Sequence Analysis Tools

See Q2RYD8 at UniProt or InterPro

Protein Sequence (662 amino acids)

>Rru_A0052 Biotin carboxylase (NCBI) (Rhodospirillum rubrum S1H)
MFDKILIANRGEIACRVIKTARKMGIKTVSVYSDADRNAVHTDMADEKVHIGGSASAQSY
LVIERIVEACKRTGAQAVHPGYGFLSENKAFQIALAKEGIAFIGPDALAIQAMGDKIESK
KLAAKAGVSTVPGYMGVIKDAEEAARIASEIGFPVMIKASAGGGGKGMRVAWSVEEAREG
FVSATNEAKSSFGDDRVFIEKFIEQPRHIEIQILADGETYLYVNERECSIQRRNQKVVEE
APSPFLTPEVRKAMGEQAVALAKVVDYKSAGTIEFIVDAARNFYFLEMNTRLQVEHPVTE
MITGLDLVEWMIRIAAGEKLTLRQEDIGIDGWAMECRIYAEDPYRNFLPSIGRLVQYAPP
EEIEGQVRVDTGVENGGEISMYYDPMIAKLVTHGADRAEAIHRMRNALDAFLIRGVAHNI
PFLASLLSRERFVEGRLTTNFIAEEYANGFNASDLPPEDPSILIAVAATVHQKGSDRNAN
ITGQIRGHEKKLKHDWVVRLNGTNHLVNVRSIDGGHEVVLEHGPVNVYHDWKFGHSLFRC
AIDGREVCVQIEREGVGYRLHHAGTQASALVLTPETAHYASLMLKKEPPDMSLYLLSPMP
GLLVSLAVEEGHEVKAGETLAVIEAMKMENILKAQHDGVIAKVHSKPGDSLSVDQKILEF
AP