Protein Info for Rru_B0046 in Rhodospirillum rubrum S1H

Annotation: Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 10 to 477 (468 residues), 711.6 bits, E=2.2e-218
• PF00483: NTP_transferase
  • amino acids 12 to 295 (284 residues), 226.9 bits, E=4.6e-71
• PF01050: MannoseP_isomer
  • amino acids 324 to 473 (150 residues), 253.7 bits, E=8.8e-80
• PF07883: Cupin_2
  • amino acids 390 to 457 (68 residues), 44.5 bits, E=1.5e-15

Best Hits

Swiss-Prot: 61% identical to XANB_XANCB: Xanthan biosynthesis protein XanB (xanB) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 100% identity to rru:Rru_B0046)

MetaCyc: 54% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

No annotation

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• D-mannose degradation II (2/2 steps found)
• mannitol degradation II (3/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• mannitol biosynthesis (1/3 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.13, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See Q2RMK3 at UniProt or InterPro

Protein Sequence (494 amino acids)

>Rru_B0046 Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (NCBI) (Rhodospirillum rubrum S1H)
MASQSASAPITPVILSGGSGSRLWPLSRALYPKQFLPLVSELTMIQETALRASGERFTAP
LVICNDEHRFIVAEQLRAAACQPTAIILEPVGRNTAPAVAVAALKLLESASDPIMLVMPS
DHVIQNADAFLAAIDQAAPAARAGALVTFGITPNAPETGYGYIKAGGSFAPFTGVLRVER
FVEKPNLQTAESYLAEGSYLWNSGIFLFSAKAYLAELGRTNSKIIEACSQALAQGQRDLS
FCRLDAAAFAASPADSIDYAVMEKTANAAVFPVEMGWSDLGAWSALWEIGPKDAQDNVAL
GDVLLQDVEGSYIRSDHRLVAVAGLCNVVVVATDDAVLVIDRGKVQDVKQIVERLKKANR
DEHALHSTVHRPWGHYRGIDRGERFQVKRIVVQPGERLSLQMHHHRAEHWIVVTGTALVT
RGAETFLLHENESTYIRAGQTHRLENPGKVPLHLIEVQSGGYLGEDDIVRFEDGYGRTLP
QKEPPLISLENKTE