Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14340 and DZA65_RS14345 are separated by 153 nucleotides DZA65_RS14345 and DZA65_RS14350 are separated by 16 nucleotides DZA65_RS14350 and DZA65_RS14355 overlap by 11 nucleotides DZA65_RS14355 and DZA65_RS14360 are separated by 141 nucleotides DZA65_RS14360 and DZA65_RS14365 are separated by 172 nucleotides
DZA65_RS14340: DZA65_RS14340 - hypothetical protein, at 3,128,873 to 3,129,787
_RS14340
DZA65_RS14345: DZA65_RS14345 - AlpA family transcriptional regulator, at 3,129,941 to 3,130,135
_RS14345
DZA65_RS14350: DZA65_RS14350 - DUF2857 domain-containing protein, at 3,130,152 to 3,130,736
_RS14350
DZA65_RS14355: DZA65_RS14355 - TraR/DksA family transcriptional regulator, at 3,130,726 to 3,130,935
_RS14355
DZA65_RS14360: DZA65_RS14360 - transposase, at 3,131,077 to 3,131,184
_RS14360
DZA65_RS14365: DZA65_RS14365 - tetratricopeptide repeat protein, at 3,131,357 to 3,135,169
_RS14365
Position (kb)
3130
3131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3129.937 kb on - strand at 3129.941 kb on - strand at 3130.011 kb on - strand, within DZA65_RS14345 at 3130.011 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.151 kb on + strand at 3130.151 kb on + strand at 3130.152 kb on - strand at 3130.152 kb on - strand at 3130.152 kb on - strand at 3130.235 kb on - strand, within DZA65_RS14350 at 3130.273 kb on + strand, within DZA65_RS14350 at 3130.320 kb on - strand, within DZA65_RS14350 at 3130.400 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.524 kb on - strand, within DZA65_RS14350 at 3130.592 kb on + strand, within DZA65_RS14350 at 3130.592 kb on + strand, within DZA65_RS14350 at 3130.683 kb on - strand at 3130.812 kb on + strand, within DZA65_RS14355 at 3130.813 kb on - strand, within DZA65_RS14355 at 3130.813 kb on - strand at 3130.823 kb on + strand, within DZA65_RS14355 at 3130.823 kb on + strand, within DZA65_RS14355 at 3130.823 kb on + strand, within DZA65_RS14355 at 3130.824 kb on - strand, within DZA65_RS14355 at 3130.829 kb on - strand, within DZA65_RS14355 at 3130.913 kb on + strand, within DZA65_RS14355 at 3130.914 kb on - strand at 3130.914 kb on - strand, within DZA65_RS14355 at 3130.947 kb on + strand at 3130.947 kb on + strand at 3131.018 kb on + strand at 3131.075 kb on - strand at 3131.201 kb on + strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.202 kb on - strand at 3131.291 kb on + strand at 3131.305 kb on - strand at 3131.362 kb on + strand at 3131.419 kb on + strand at 3131.703 kb on + strand at 3131.719 kb on + strand at 3131.719 kb on + strand at 3131.732 kb on + strand at 3131.732 kb on + strand at 3131.785 kb on + strand, within DZA65_RS14365 at 3131.790 kb on - strand, within DZA65_RS14365 at 3131.820 kb on + strand, within DZA65_RS14365 at 3131.820 kb on + strand, within DZA65_RS14365 at 3131.898 kb on - strand, within DZA65_RS14365 at 3131.903 kb on - strand, within DZA65_RS14365 at 3131.903 kb on - strand, within DZA65_RS14365 at 3131.903 kb on - strand, within DZA65_RS14365 at 3131.935 kb on + strand, within DZA65_RS14365
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,129,937 - -0.1 3,129,941 - +0.5 3,130,011 - DZA65_RS14345 0.36 +0.5 3,130,011 - DZA65_RS14345 0.36 +0.2 3,130,017 - DZA65_RS14345 0.39 +0.2 3,130,017 - DZA65_RS14345 0.39 -0.4 3,130,017 - DZA65_RS14345 0.39 -1.4 3,130,017 - DZA65_RS14345 0.39 -1.5 3,130,151 + -2.8 3,130,151 + -1.5 3,130,152 - +0.1 3,130,152 - +0.3 3,130,152 - +1.1 3,130,235 - DZA65_RS14350 0.14 +0.5 3,130,273 + DZA65_RS14350 0.21 -1.2 3,130,320 - DZA65_RS14350 0.29 -0.6 3,130,400 + DZA65_RS14350 0.42 -1.2 3,130,427 + DZA65_RS14350 0.47 -2.1 3,130,427 + DZA65_RS14350 0.47 -1.8 3,130,427 + DZA65_RS14350 0.47 -0.5 3,130,427 + DZA65_RS14350 0.47 -2.4 3,130,428 - DZA65_RS14350 0.47 -0.3 3,130,428 - DZA65_RS14350 0.47 -0.8 3,130,428 - DZA65_RS14350 0.47 -0.2 3,130,524 - DZA65_RS14350 0.64 -0.2 3,130,592 + DZA65_RS14350 0.75 +0.5 3,130,592 + DZA65_RS14350 0.75 -2.9 3,130,683 - -0.9 3,130,812 + DZA65_RS14355 0.41 -2.0 3,130,813 - DZA65_RS14355 0.41 -1.1 3,130,813 - +3.1 3,130,823 + DZA65_RS14355 0.46 -0.2 3,130,823 + DZA65_RS14355 0.46 +1.6 3,130,823 + DZA65_RS14355 0.46 -0.7 3,130,824 - DZA65_RS14355 0.47 -0.4 3,130,829 - DZA65_RS14355 0.49 -0.6 3,130,913 + DZA65_RS14355 0.89 -0.2 3,130,914 - -0.2 3,130,914 - DZA65_RS14355 0.90 -2.1 3,130,947 + -1.1 3,130,947 + -0.6 3,131,018 + -0.0 3,131,075 - -0.4 3,131,201 + +0.1 3,131,202 - -0.0 3,131,202 - -0.5 3,131,202 - -1.8 3,131,202 - +0.2 3,131,202 - +1.2 3,131,202 - +1.0 3,131,202 - -2.8 3,131,202 - +0.2 3,131,291 + +0.5 3,131,305 - -0.2 3,131,362 + +0.2 3,131,419 + -1.0 3,131,703 + -0.1 3,131,719 + -1.9 3,131,719 + +2.1 3,131,732 + -0.7 3,131,732 + -2.0 3,131,785 + DZA65_RS14365 0.11 +0.3 3,131,790 - DZA65_RS14365 0.11 +0.4 3,131,820 + DZA65_RS14365 0.12 -0.7 3,131,820 + DZA65_RS14365 0.12 +0.0 3,131,898 - DZA65_RS14365 0.14 +0.8 3,131,903 - DZA65_RS14365 0.14 +0.4 3,131,903 - DZA65_RS14365 0.14 -1.0 3,131,903 - DZA65_RS14365 0.14 -0.2 3,131,935 + DZA65_RS14365 0.15 -0.3
Or see this region's nucleotide sequence