Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01230 and DZA65_RS01235 overlap by 4 nucleotides DZA65_RS01235 and DZA65_RS01240 are separated by 91 nucleotides DZA65_RS01240 and DZA65_RS01245 are separated by 311 nucleotides
DZA65_RS01230: DZA65_RS01230 - uroporphyrinogen decarboxylase, at 274,754 to 275,827
_RS01230
DZA65_RS01235: DZA65_RS01235 - deoxyribonuclease V, at 275,824 to 276,501
_RS01235
DZA65_RS01240: DZA65_RS01240 - YjaG family protein, at 276,593 to 277,183
_RS01240
DZA65_RS01245: DZA65_RS01245 - DNA-binding protein HU-alpha, at 277,495 to 277,767
_RS01245
Position (kb) 275
276
277 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 275.808 kb on - strand at 275.833 kb on - strand at 275.892 kb on + strand, within DZA65_RS01235 at 275.892 kb on + strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.893 kb on - strand, within DZA65_RS01235 at 275.986 kb on + strand, within DZA65_RS01235 at 276.011 kb on - strand, within DZA65_RS01235 at 276.025 kb on - strand, within DZA65_RS01235 at 276.033 kb on + strand, within DZA65_RS01235 at 276.034 kb on - strand at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.043 kb on + strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.044 kb on - strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.045 kb on + strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.046 kb on - strand, within DZA65_RS01235 at 276.363 kb on + strand, within DZA65_RS01235 at 276.500 kb on - strand at 276.536 kb on + strand at 276.536 kb on + strand at 276.536 kb on + strand at 276.537 kb on - strand at 276.537 kb on - strand at 276.538 kb on + strand at 276.562 kb on - strand at 276.573 kb on + strand at 276.573 kb on + strand at 276.576 kb on + strand at 276.576 kb on + strand at 276.576 kb on + strand at 276.576 kb on + strand at 276.577 kb on - strand at 276.577 kb on - strand at 276.577 kb on - strand at 276.579 kb on + strand at 276.579 kb on + strand at 276.579 kb on + strand at 276.580 kb on - strand at 276.580 kb on - strand at 276.587 kb on + strand at 276.597 kb on + strand at 276.597 kb on + strand at 276.597 kb on + strand at 276.597 kb on + strand at 276.598 kb on - strand at 276.598 kb on - strand at 276.598 kb on - strand at 276.598 kb on - strand at 276.598 kb on - strand at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.676 kb on + strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.677 kb on - strand, within DZA65_RS01240 at 276.680 kb on + strand, within DZA65_RS01240 at 276.680 kb on + strand, within DZA65_RS01240 at 276.680 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.689 kb on + strand, within DZA65_RS01240 at 276.690 kb on - strand at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.690 kb on - strand, within DZA65_RS01240 at 276.692 kb on + strand, within DZA65_RS01240 at 276.692 kb on + strand, within DZA65_RS01240 at 276.692 kb on + strand, within DZA65_RS01240 at 276.693 kb on - strand, within DZA65_RS01240 at 276.742 kb on + strand, within DZA65_RS01240 at 276.854 kb on + strand, within DZA65_RS01240 at 276.854 kb on + strand, within DZA65_RS01240 at 276.855 kb on - strand, within DZA65_RS01240 at 276.855 kb on - strand, within DZA65_RS01240 at 276.855 kb on - strand, within DZA65_RS01240 at 276.861 kb on + strand, within DZA65_RS01240 at 276.861 kb on + strand, within DZA65_RS01240 at 276.861 kb on + strand, within DZA65_RS01240 at 276.862 kb on - strand, within DZA65_RS01240 at 276.862 kb on - strand, within DZA65_RS01240 at 276.862 kb on - strand, within DZA65_RS01240 at 276.862 kb on - strand, within DZA65_RS01240 at 276.972 kb on + strand, within DZA65_RS01240 at 277.117 kb on - strand, within DZA65_RS01240 at 277.117 kb on - strand, within DZA65_RS01240 at 277.153 kb on + strand at 277.153 kb on + strand at 277.154 kb on - strand at 277.154 kb on - strand at 277.154 kb on - strand at 277.157 kb on - strand at 277.175 kb on + strand at 277.201 kb on + strand at 277.202 kb on - strand at 277.202 kb on - strand at 277.202 kb on - strand at 277.224 kb on + strand at 277.253 kb on + strand at 277.253 kb on + strand at 277.253 kb on + strand at 277.268 kb on + strand at 277.293 kb on + strand at 277.293 kb on + strand at 277.293 kb on + strand at 277.369 kb on + strand at 277.369 kb on + strand at 277.377 kb on + strand at 277.377 kb on + strand at 277.377 kb on + strand at 277.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 275,808 - +0.1 275,833 - -0.2 275,892 + DZA65_RS01235 0.10 +0.1 275,892 + DZA65_RS01235 0.10 -0.3 275,893 - DZA65_RS01235 0.10 +0.6 275,893 - DZA65_RS01235 0.10 -0.3 275,893 - DZA65_RS01235 0.10 +1.6 275,893 - DZA65_RS01235 0.10 -0.5 275,893 - DZA65_RS01235 0.10 -0.1 275,893 - DZA65_RS01235 0.10 -0.3 275,893 - DZA65_RS01235 0.10 -0.8 275,893 - DZA65_RS01235 0.10 +4.9 275,986 + DZA65_RS01235 0.24 -0.5 276,011 - DZA65_RS01235 0.28 +0.3 276,025 - DZA65_RS01235 0.30 -0.6 276,033 + DZA65_RS01235 0.31 +0.6 276,034 - +1.3 276,043 + DZA65_RS01235 0.32 +1.3 276,043 + DZA65_RS01235 0.32 -1.0 276,043 + DZA65_RS01235 0.32 -0.1 276,043 + DZA65_RS01235 0.32 -0.9 276,043 + DZA65_RS01235 0.32 -0.5 276,043 + DZA65_RS01235 0.32 -0.0 276,043 + DZA65_RS01235 0.32 -0.1 276,043 + DZA65_RS01235 0.32 -1.7 276,043 + DZA65_RS01235 0.32 -0.4 276,043 + DZA65_RS01235 0.32 +0.1 276,043 + DZA65_RS01235 0.32 +0.7 276,043 + DZA65_RS01235 0.32 -0.3 276,043 + DZA65_RS01235 0.32 +0.4 276,043 + DZA65_RS01235 0.32 -0.0 276,043 + DZA65_RS01235 0.32 +2.9 276,043 + DZA65_RS01235 0.32 +0.7 276,043 + DZA65_RS01235 0.32 -0.1 276,043 + DZA65_RS01235 0.32 +1.3 276,043 + DZA65_RS01235 0.32 -1.1 276,043 + DZA65_RS01235 0.32 +0.6 276,043 + DZA65_RS01235 0.32 -1.4 276,043 + DZA65_RS01235 0.32 +0.3 276,044 - DZA65_RS01235 0.32 -0.2 276,044 - DZA65_RS01235 0.32 +0.5 276,044 - DZA65_RS01235 0.32 +1.1 276,044 - DZA65_RS01235 0.32 -0.6 276,044 - DZA65_RS01235 0.32 +0.5 276,044 - DZA65_RS01235 0.32 -1.3 276,044 - DZA65_RS01235 0.32 +0.1 276,044 - DZA65_RS01235 0.32 +0.7 276,044 - DZA65_RS01235 0.32 +0.1 276,044 - DZA65_RS01235 0.32 -1.4 276,044 - DZA65_RS01235 0.32 +0.3 276,044 - DZA65_RS01235 0.32 -0.3 276,044 - DZA65_RS01235 0.32 -0.1 276,044 - DZA65_RS01235 0.32 +0.2 276,044 - DZA65_RS01235 0.32 +0.6 276,044 - DZA65_RS01235 0.32 +2.9 276,044 - DZA65_RS01235 0.32 -0.3 276,045 + DZA65_RS01235 0.33 +1.2 276,045 + DZA65_RS01235 0.33 +0.2 276,045 + DZA65_RS01235 0.33 -0.6 276,045 + DZA65_RS01235 0.33 +0.0 276,045 + DZA65_RS01235 0.33 +1.9 276,045 + DZA65_RS01235 0.33 -0.2 276,046 - DZA65_RS01235 0.33 -0.1 276,046 - DZA65_RS01235 0.33 -1.3 276,046 - DZA65_RS01235 0.33 +0.1 276,046 - DZA65_RS01235 0.33 +0.2 276,046 - DZA65_RS01235 0.33 -0.6 276,046 - DZA65_RS01235 0.33 -1.4 276,363 + DZA65_RS01235 0.79 +0.3 276,500 - -1.5 276,536 + +1.2 276,536 + +1.3 276,536 + +0.1 276,537 - +1.5 276,537 - +0.6 276,538 + +0.3 276,562 - -1.1 276,573 + +0.0 276,573 + +1.5 276,576 + -0.0 276,576 + -0.3 276,576 + +1.1 276,576 + +0.8 276,577 - +1.3 276,577 - +1.7 276,577 - -0.5 276,579 + +0.9 276,579 + -0.3 276,579 + -0.5 276,580 - +0.5 276,580 - -0.3 276,587 + +0.4 276,597 + -2.0 276,597 + +0.5 276,597 + -0.5 276,597 + +0.2 276,598 - -0.0 276,598 - -0.8 276,598 - -1.0 276,598 - -0.0 276,598 - -0.3 276,676 + DZA65_RS01240 0.14 +0.6 276,676 + +0.7 276,676 + DZA65_RS01240 0.14 -1.5 276,676 + DZA65_RS01240 0.14 -0.9 276,676 + DZA65_RS01240 0.14 -1.3 276,676 + DZA65_RS01240 0.14 +0.5 276,676 + DZA65_RS01240 0.14 -0.9 276,676 + DZA65_RS01240 0.14 -1.0 276,676 + DZA65_RS01240 0.14 -0.9 276,676 + DZA65_RS01240 0.14 -2.5 276,676 + DZA65_RS01240 0.14 -0.6 276,676 + DZA65_RS01240 0.14 -0.7 276,676 + DZA65_RS01240 0.14 +0.9 276,676 + DZA65_RS01240 0.14 -1.1 276,676 + DZA65_RS01240 0.14 -1.3 276,676 + DZA65_RS01240 0.14 -2.0 276,676 + DZA65_RS01240 0.14 -0.3 276,677 - DZA65_RS01240 0.14 -0.3 276,677 - DZA65_RS01240 0.14 +0.6 276,677 - DZA65_RS01240 0.14 -0.4 276,677 - DZA65_RS01240 0.14 +0.5 276,677 - DZA65_RS01240 0.14 -0.1 276,677 - DZA65_RS01240 0.14 -0.6 276,677 - DZA65_RS01240 0.14 +0.8 276,677 - DZA65_RS01240 0.14 -1.3 276,677 - -1.0 276,677 - DZA65_RS01240 0.14 -1.0 276,677 - DZA65_RS01240 0.14 -2.3 276,677 - DZA65_RS01240 0.14 -0.1 276,677 - DZA65_RS01240 0.14 -0.7 276,677 - DZA65_RS01240 0.14 -0.3 276,677 - DZA65_RS01240 0.14 -0.7 276,677 - DZA65_RS01240 0.14 -0.0 276,680 + DZA65_RS01240 0.15 -1.0 276,680 + DZA65_RS01240 0.15 -0.6 276,680 + DZA65_RS01240 0.15 -1.4 276,689 + DZA65_RS01240 0.16 +0.1 276,689 + DZA65_RS01240 0.16 -0.0 276,689 + DZA65_RS01240 0.16 +0.3 276,689 + DZA65_RS01240 0.16 -0.7 276,689 + DZA65_RS01240 0.16 -0.4 276,689 + DZA65_RS01240 0.16 -0.3 276,690 - +1.3 276,690 - DZA65_RS01240 0.16 -1.0 276,690 - DZA65_RS01240 0.16 -1.3 276,690 - DZA65_RS01240 0.16 -2.1 276,690 - DZA65_RS01240 0.16 +0.9 276,690 - DZA65_RS01240 0.16 -1.0 276,690 - DZA65_RS01240 0.16 +0.2 276,690 - DZA65_RS01240 0.16 -0.3 276,690 - DZA65_RS01240 0.16 +0.2 276,692 + DZA65_RS01240 0.17 +1.1 276,692 + DZA65_RS01240 0.17 +0.1 276,692 + DZA65_RS01240 0.17 -0.5 276,693 - DZA65_RS01240 0.17 -0.0 276,742 + DZA65_RS01240 0.25 +0.1 276,854 + DZA65_RS01240 0.44 -0.7 276,854 + DZA65_RS01240 0.44 -0.5 276,855 - DZA65_RS01240 0.44 +0.5 276,855 - DZA65_RS01240 0.44 -1.5 276,855 - DZA65_RS01240 0.44 +0.7 276,861 + DZA65_RS01240 0.45 -1.9 276,861 + DZA65_RS01240 0.45 -0.0 276,861 + DZA65_RS01240 0.45 -0.7 276,862 - DZA65_RS01240 0.46 -0.0 276,862 - DZA65_RS01240 0.46 +0.9 276,862 - DZA65_RS01240 0.46 -2.6 276,862 - DZA65_RS01240 0.46 -0.8 276,972 + DZA65_RS01240 0.64 -1.0 277,117 - DZA65_RS01240 0.89 -0.2 277,117 - DZA65_RS01240 0.89 -0.6 277,153 + -0.0 277,153 + -0.6 277,154 - +0.1 277,154 - -0.3 277,154 - +0.1 277,157 - +1.3 277,175 + -0.1 277,201 + -1.4 277,202 - -0.5 277,202 - -0.9 277,202 - -0.7 277,224 + -0.2 277,253 + +0.1 277,253 + +0.5 277,253 + +0.0 277,268 + +0.8 277,293 + +0.9 277,293 + -0.4 277,293 + -2.0 277,369 + -0.4 277,369 + -0.3 277,377 + -1.9 277,377 + +1.1 277,377 + +1.6 277,425 + +1.5
Or see this region's nucleotide sequence