Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00235 and DZA65_RS00240 are separated by 2 nucleotides DZA65_RS00240 and DZA65_RS00245 are separated by 28 nucleotides DZA65_RS00245 and DZA65_RS00250 are separated by 27 nucleotides DZA65_RS00250 and DZA65_RS00255 are separated by 16 nucleotides
DZA65_RS00235: DZA65_RS00235 - urea carboxylase-associated family protein, at 57,368 to 58,003
_RS00235
DZA65_RS00240: DZA65_RS00240 - urea carboxylase-associated family protein, at 58,006 to 58,725
_RS00240
DZA65_RS00245: DZA65_RS00245 - ABC transporter ATP-binding protein, at 58,754 to 59,530
_RS00245
DZA65_RS00250: DZA65_RS00250 - ABC transporter permease subunit, at 59,558 to 60,370
_RS00250
DZA65_RS00255: DZA65_RS00255 - ABC transporter substrate-binding protein, at 60,387 to 61,442
_RS00255
Position (kb) 58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 57.855 kb on + strand, within DZA65_RS00235 at 57.855 kb on + strand, within DZA65_RS00235 at 57.855 kb on + strand, within DZA65_RS00235 at 57.855 kb on + strand, within DZA65_RS00235 at 57.855 kb on + strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.856 kb on - strand, within DZA65_RS00235 at 57.880 kb on + strand, within DZA65_RS00235 at 57.881 kb on - strand, within DZA65_RS00235 at 57.899 kb on - strand, within DZA65_RS00235 at 57.899 kb on - strand, within DZA65_RS00235 at 57.899 kb on - strand, within DZA65_RS00235 at 57.899 kb on - strand, within DZA65_RS00235 at 57.899 kb on - strand, within DZA65_RS00235 at 58.034 kb on + strand at 58.034 kb on + strand at 58.034 kb on + strand at 58.034 kb on + strand at 58.034 kb on + strand at 58.034 kb on + strand at 58.035 kb on - strand at 58.037 kb on + strand at 58.037 kb on + strand at 58.037 kb on + strand at 58.037 kb on + strand at 58.038 kb on - strand at 58.038 kb on - strand at 58.038 kb on - strand at 58.038 kb on - strand at 58.038 kb on - strand at 58.038 kb on - strand at 58.203 kb on + strand, within DZA65_RS00240 at 58.204 kb on - strand, within DZA65_RS00240 at 58.332 kb on - strand, within DZA65_RS00240 at 58.332 kb on - strand, within DZA65_RS00240 at 58.397 kb on + strand, within DZA65_RS00240 at 58.398 kb on - strand, within DZA65_RS00240 at 58.398 kb on - strand, within DZA65_RS00240 at 58.398 kb on - strand, within DZA65_RS00240 at 58.398 kb on - strand, within DZA65_RS00240 at 58.497 kb on + strand, within DZA65_RS00240 at 58.497 kb on + strand, within DZA65_RS00240 at 58.498 kb on - strand, within DZA65_RS00240 at 58.498 kb on - strand, within DZA65_RS00240 at 58.498 kb on - strand, within DZA65_RS00240 at 58.515 kb on - strand, within DZA65_RS00240 at 58.515 kb on - strand, within DZA65_RS00240 at 58.577 kb on + strand, within DZA65_RS00240 at 58.577 kb on + strand, within DZA65_RS00240 at 58.577 kb on + strand, within DZA65_RS00240 at 58.578 kb on - strand, within DZA65_RS00240 at 58.602 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.604 kb on + strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.605 kb on - strand, within DZA65_RS00240 at 58.712 kb on + strand at 58.712 kb on + strand at 58.712 kb on + strand at 58.712 kb on + strand at 58.713 kb on - strand at 58.713 kb on - strand at 58.713 kb on - strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.737 kb on + strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.738 kb on - strand at 58.833 kb on + strand, within DZA65_RS00245 at 58.833 kb on + strand, within DZA65_RS00245 at 58.833 kb on + strand, within DZA65_RS00245 at 58.834 kb on - strand, within DZA65_RS00245 at 58.834 kb on - strand, within DZA65_RS00245 at 58.834 kb on - strand, within DZA65_RS00245 at 58.834 kb on - strand, within DZA65_RS00245 at 58.834 kb on - strand, within DZA65_RS00245 at 58.973 kb on + strand, within DZA65_RS00245 at 59.121 kb on + strand, within DZA65_RS00245 at 59.121 kb on + strand, within DZA65_RS00245 at 59.121 kb on + strand, within DZA65_RS00245 at 59.122 kb on - strand, within DZA65_RS00245 at 59.122 kb on - strand, within DZA65_RS00245 at 59.125 kb on + strand, within DZA65_RS00245 at 59.125 kb on + strand, within DZA65_RS00245 at 59.125 kb on + strand, within DZA65_RS00245 at 59.125 kb on + strand, within DZA65_RS00245 at 59.126 kb on - strand, within DZA65_RS00245 at 59.126 kb on - strand, within DZA65_RS00245 at 59.286 kb on + strand, within DZA65_RS00245 at 59.493 kb on + strand at 59.494 kb on - strand at 59.513 kb on - strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.531 kb on - strand at 59.531 kb on - strand at 59.571 kb on + strand at 59.571 kb on + strand at 59.571 kb on + strand at 59.572 kb on - strand at 59.572 kb on - strand at 59.572 kb on - strand at 59.572 kb on - strand at 59.572 kb on - strand at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.649 kb on + strand, within DZA65_RS00250 at 59.650 kb on - strand, within DZA65_RS00250 at 59.650 kb on - strand, within DZA65_RS00250 at 59.650 kb on - strand, within DZA65_RS00250 at 59.650 kb on - strand, within DZA65_RS00250 at 59.676 kb on + strand, within DZA65_RS00250 at 59.812 kb on + strand, within DZA65_RS00250 at 59.812 kb on + strand, within DZA65_RS00250 at 59.813 kb on - strand, within DZA65_RS00250 at 59.813 kb on - strand, within DZA65_RS00250 at 59.813 kb on - strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.892 kb on + strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.893 kb on - strand, within DZA65_RS00250 at 59.954 kb on + strand, within DZA65_RS00250 at 59.954 kb on + strand, within DZA65_RS00250 at 59.955 kb on - strand, within DZA65_RS00250 at 60.105 kb on - strand, within DZA65_RS00250 at 60.285 kb on + strand, within DZA65_RS00250 at 60.286 kb on - strand, within DZA65_RS00250 at 60.286 kb on - strand, within DZA65_RS00250 at 60.305 kb on + strand at 60.351 kb on + strand at 60.351 kb on + strand at 60.352 kb on - strand at 60.352 kb on - strand at 60.352 kb on - strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.388 kb on + strand at 60.389 kb on - strand at 60.389 kb on - strand at 60.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 57,855 + DZA65_RS00235 0.77 +0.0 57,855 + DZA65_RS00235 0.77 -0.5 57,855 + DZA65_RS00235 0.77 -0.5 57,855 + DZA65_RS00235 0.77 -0.4 57,855 + DZA65_RS00235 0.77 -1.5 57,856 - DZA65_RS00235 0.77 -0.4 57,856 - DZA65_RS00235 0.77 -0.0 57,856 - DZA65_RS00235 0.77 +1.4 57,856 - DZA65_RS00235 0.77 -0.1 57,856 - DZA65_RS00235 0.77 -0.6 57,856 - DZA65_RS00235 0.77 -0.2 57,856 - DZA65_RS00235 0.77 +0.0 57,880 + DZA65_RS00235 0.81 -0.6 57,881 - DZA65_RS00235 0.81 -1.2 57,899 - DZA65_RS00235 0.83 +0.2 57,899 - DZA65_RS00235 0.83 +0.4 57,899 - DZA65_RS00235 0.83 +1.0 57,899 - DZA65_RS00235 0.83 +0.0 57,899 - DZA65_RS00235 0.83 -0.8 58,034 + +0.2 58,034 + -0.4 58,034 + -0.5 58,034 + -0.1 58,034 + -0.6 58,034 + -1.3 58,035 - -0.6 58,037 + -0.4 58,037 + +0.0 58,037 + +1.2 58,037 + -0.0 58,038 - +0.5 58,038 - +0.4 58,038 - -0.8 58,038 - +0.6 58,038 - +1.9 58,038 - -0.2 58,203 + DZA65_RS00240 0.27 -0.3 58,204 - DZA65_RS00240 0.28 +0.3 58,332 - DZA65_RS00240 0.45 +0.6 58,332 - DZA65_RS00240 0.45 -0.6 58,397 + DZA65_RS00240 0.54 -2.1 58,398 - DZA65_RS00240 0.54 +0.1 58,398 - DZA65_RS00240 0.54 -1.2 58,398 - DZA65_RS00240 0.54 -0.3 58,398 - DZA65_RS00240 0.54 +0.7 58,497 + DZA65_RS00240 0.68 -1.5 58,497 + DZA65_RS00240 0.68 -2.8 58,498 - DZA65_RS00240 0.68 +0.4 58,498 - DZA65_RS00240 0.68 -0.6 58,498 - DZA65_RS00240 0.68 -0.2 58,515 - DZA65_RS00240 0.71 -1.2 58,515 - DZA65_RS00240 0.71 -0.4 58,577 + DZA65_RS00240 0.79 -1.8 58,577 + DZA65_RS00240 0.79 -0.6 58,577 + DZA65_RS00240 0.79 +0.8 58,578 - DZA65_RS00240 0.79 -0.2 58,602 + DZA65_RS00240 0.83 +0.5 58,604 + DZA65_RS00240 0.83 -0.2 58,604 + DZA65_RS00240 0.83 -0.2 58,604 + DZA65_RS00240 0.83 -0.6 58,604 + DZA65_RS00240 0.83 -1.1 58,604 + DZA65_RS00240 0.83 +0.2 58,604 + DZA65_RS00240 0.83 -0.1 58,604 + DZA65_RS00240 0.83 +0.3 58,604 + DZA65_RS00240 0.83 +0.8 58,605 - DZA65_RS00240 0.83 +0.8 58,605 - DZA65_RS00240 0.83 -0.0 58,605 - DZA65_RS00240 0.83 +0.2 58,605 - DZA65_RS00240 0.83 +0.2 58,605 - DZA65_RS00240 0.83 +0.4 58,605 - DZA65_RS00240 0.83 -1.5 58,605 - DZA65_RS00240 0.83 -0.2 58,712 + +0.1 58,712 + +0.4 58,712 + +1.4 58,712 + -1.3 58,713 - +0.0 58,713 - +0.0 58,713 - +0.6 58,737 + +0.7 58,737 + -0.6 58,737 + -0.8 58,737 + +0.5 58,737 + -0.3 58,737 + +0.5 58,737 + -1.6 58,738 - +0.9 58,738 - -0.2 58,738 - +0.3 58,738 - -0.4 58,738 - +1.2 58,738 - +0.1 58,738 - -0.2 58,738 - +0.4 58,738 - -0.2 58,738 - -0.5 58,738 - -0.2 58,738 - -0.3 58,738 - +0.0 58,833 + DZA65_RS00245 0.10 -0.2 58,833 + DZA65_RS00245 0.10 -0.1 58,833 + DZA65_RS00245 0.10 -0.4 58,834 - DZA65_RS00245 0.10 -1.1 58,834 - DZA65_RS00245 0.10 -0.0 58,834 - DZA65_RS00245 0.10 +0.3 58,834 - DZA65_RS00245 0.10 -0.1 58,834 - DZA65_RS00245 0.10 -1.0 58,973 + DZA65_RS00245 0.28 -0.5 59,121 + DZA65_RS00245 0.47 -1.3 59,121 + DZA65_RS00245 0.47 -1.0 59,121 + DZA65_RS00245 0.47 +0.6 59,122 - DZA65_RS00245 0.47 -1.1 59,122 - DZA65_RS00245 0.47 -0.4 59,125 + DZA65_RS00245 0.48 +0.6 59,125 + DZA65_RS00245 0.48 -0.2 59,125 + DZA65_RS00245 0.48 +1.1 59,125 + DZA65_RS00245 0.48 +0.1 59,126 - DZA65_RS00245 0.48 +0.4 59,126 - DZA65_RS00245 0.48 -0.8 59,286 + DZA65_RS00245 0.68 +0.5 59,493 + +1.1 59,494 - -0.8 59,513 - -0.9 59,530 + -0.6 59,530 + -0.3 59,530 + -0.0 59,531 - +1.4 59,531 - -0.3 59,571 + -0.4 59,571 + -2.0 59,571 + +0.2 59,572 - -0.9 59,572 - +0.2 59,572 - -0.9 59,572 - -0.2 59,572 - +0.8 59,649 + DZA65_RS00250 0.11 +0.8 59,649 + DZA65_RS00250 0.11 -0.6 59,649 + DZA65_RS00250 0.11 -0.2 59,649 + DZA65_RS00250 0.11 -2.0 59,649 + DZA65_RS00250 0.11 -0.6 59,649 + DZA65_RS00250 0.11 +0.2 59,649 + DZA65_RS00250 0.11 +0.5 59,650 - DZA65_RS00250 0.11 +0.8 59,650 - DZA65_RS00250 0.11 +0.3 59,650 - DZA65_RS00250 0.11 -0.5 59,650 - DZA65_RS00250 0.11 -0.1 59,676 + DZA65_RS00250 0.15 -0.2 59,812 + DZA65_RS00250 0.31 -1.1 59,812 + DZA65_RS00250 0.31 +0.4 59,813 - DZA65_RS00250 0.31 +0.1 59,813 - DZA65_RS00250 0.31 -1.0 59,813 - DZA65_RS00250 0.31 -1.2 59,892 + DZA65_RS00250 0.41 -0.7 59,892 + DZA65_RS00250 0.41 -0.5 59,892 + DZA65_RS00250 0.41 +0.2 59,892 + DZA65_RS00250 0.41 -0.2 59,892 + DZA65_RS00250 0.41 +0.4 59,892 + DZA65_RS00250 0.41 -0.9 59,892 + DZA65_RS00250 0.41 +1.4 59,893 - DZA65_RS00250 0.41 -0.2 59,893 - DZA65_RS00250 0.41 -0.2 59,893 - DZA65_RS00250 0.41 -1.2 59,893 - DZA65_RS00250 0.41 -0.4 59,893 - DZA65_RS00250 0.41 -0.1 59,893 - DZA65_RS00250 0.41 +0.4 59,893 - DZA65_RS00250 0.41 +0.2 59,893 - DZA65_RS00250 0.41 -0.2 59,893 - DZA65_RS00250 0.41 +0.3 59,893 - DZA65_RS00250 0.41 -0.9 59,893 - DZA65_RS00250 0.41 +0.5 59,893 - DZA65_RS00250 0.41 +0.1 59,893 - DZA65_RS00250 0.41 -1.0 59,893 - DZA65_RS00250 0.41 +1.0 59,893 - DZA65_RS00250 0.41 +1.0 59,893 - DZA65_RS00250 0.41 +0.6 59,954 + DZA65_RS00250 0.49 -0.3 59,954 + DZA65_RS00250 0.49 +0.1 59,955 - DZA65_RS00250 0.49 -1.2 60,105 - DZA65_RS00250 0.67 -0.2 60,285 + DZA65_RS00250 0.89 +0.9 60,286 - DZA65_RS00250 0.90 -0.4 60,286 - DZA65_RS00250 0.90 -0.3 60,305 + +0.4 60,351 + -0.2 60,351 + +1.1 60,352 - +0.2 60,352 - -0.1 60,352 - -0.1 60,388 + -0.6 60,388 + -0.6 60,388 + +0.0 60,388 + -1.6 60,388 + -0.3 60,388 + +0.0 60,388 + +1.8 60,389 - -0.5 60,389 - +0.0 60,389 - +1.4
Or see this region's nucleotide sequence