Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS21030 and DZA65_RS21035 are separated by 134 nucleotides DZA65_RS21035 and DZA65_RS21040 are separated by 122 nucleotides DZA65_RS21040 and DZA65_RS21045 are separated by 77 nucleotides
DZA65_RS21030: DZA65_RS21030 - lysophospholipase L2, at 4,548,171 to 4,549,163
_RS21030
DZA65_RS21035: DZA65_RS21035 - homoserine/homoserine lactone efflux protein, at 4,549,298 to 4,549,918
_RS21035
DZA65_RS21040: DZA65_RS21040 - threonine export protein RhtC, at 4,550,041 to 4,550,664
_RS21040
DZA65_RS21045: DZA65_RS21045 - ATP-dependent DNA helicase RecQ, at 4,550,742 to 4,552,571
_RS21045
Position (kb)
4549
4550 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.451 kb on + strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.452 kb on - strand, within DZA65_RS21030 at 4548.490 kb on + strand, within DZA65_RS21030 at 4548.490 kb on + strand, within DZA65_RS21030 at 4548.491 kb on - strand, within DZA65_RS21030 at 4548.491 kb on - strand, within DZA65_RS21030 at 4548.546 kb on - strand, within DZA65_RS21030 at 4548.583 kb on + strand, within DZA65_RS21030 at 4548.583 kb on + strand, within DZA65_RS21030 at 4548.583 kb on + strand, within DZA65_RS21030 at 4548.584 kb on - strand, within DZA65_RS21030 at 4548.584 kb on - strand, within DZA65_RS21030 at 4548.584 kb on - strand, within DZA65_RS21030 at 4548.584 kb on - strand, within DZA65_RS21030 at 4548.584 kb on - strand, within DZA65_RS21030 at 4548.586 kb on + strand, within DZA65_RS21030 at 4548.586 kb on + strand, within DZA65_RS21030 at 4548.586 kb on + strand, within DZA65_RS21030 at 4548.586 kb on + strand, within DZA65_RS21030 at 4548.586 kb on + strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.587 kb on - strand, within DZA65_RS21030 at 4548.773 kb on + strand, within DZA65_RS21030 at 4548.773 kb on + strand, within DZA65_RS21030 at 4548.774 kb on - strand, within DZA65_RS21030 at 4548.774 kb on - strand, within DZA65_RS21030 at 4548.776 kb on - strand, within DZA65_RS21030 at 4548.826 kb on + strand, within DZA65_RS21030 at 4548.826 kb on + strand, within DZA65_RS21030 at 4548.826 kb on + strand, within DZA65_RS21030 at 4548.826 kb on + strand, within DZA65_RS21030 at 4548.827 kb on - strand, within DZA65_RS21030 at 4548.827 kb on - strand, within DZA65_RS21030 at 4548.827 kb on - strand, within DZA65_RS21030 at 4548.842 kb on + strand, within DZA65_RS21030 at 4548.842 kb on + strand, within DZA65_RS21030 at 4548.842 kb on + strand, within DZA65_RS21030 at 4548.843 kb on - strand, within DZA65_RS21030 at 4548.843 kb on - strand, within DZA65_RS21030 at 4548.941 kb on - strand, within DZA65_RS21030 at 4548.955 kb on + strand, within DZA65_RS21030 at 4548.956 kb on - strand, within DZA65_RS21030 at 4548.956 kb on - strand, within DZA65_RS21030 at 4548.964 kb on + strand, within DZA65_RS21030 at 4548.964 kb on + strand, within DZA65_RS21030 at 4548.964 kb on + strand, within DZA65_RS21030 at 4548.964 kb on + strand, within DZA65_RS21030 at 4548.964 kb on + strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand, within DZA65_RS21030 at 4548.965 kb on - strand at 4549.041 kb on + strand, within DZA65_RS21030 at 4549.041 kb on + strand, within DZA65_RS21030 at 4549.041 kb on + strand, within DZA65_RS21030 at 4549.041 kb on + strand, within DZA65_RS21030 at 4549.042 kb on - strand, within DZA65_RS21030 at 4549.042 kb on - strand, within DZA65_RS21030 at 4549.042 kb on - strand, within DZA65_RS21030 at 4549.179 kb on + strand at 4549.180 kb on - strand at 4549.212 kb on + strand at 4549.233 kb on + strand at 4549.233 kb on + strand at 4549.233 kb on + strand at 4549.233 kb on + strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.234 kb on - strand at 4549.235 kb on + strand at 4549.235 kb on + strand at 4549.269 kb on - strand at 4549.323 kb on - strand at 4549.323 kb on - strand at 4549.323 kb on - strand at 4549.323 kb on - strand at 4549.338 kb on + strand at 4549.338 kb on + strand at 4549.338 kb on + strand at 4549.338 kb on + strand at 4549.338 kb on + strand at 4549.338 kb on + strand at 4549.339 kb on - strand at 4549.339 kb on - strand at 4549.339 kb on - strand at 4549.516 kb on + strand, within DZA65_RS21035 at 4549.516 kb on + strand, within DZA65_RS21035 at 4549.541 kb on + strand, within DZA65_RS21035 at 4549.541 kb on + strand, within DZA65_RS21035 at 4549.542 kb on - strand, within DZA65_RS21035 at 4549.612 kb on + strand, within DZA65_RS21035 at 4549.705 kb on + strand, within DZA65_RS21035 at 4549.705 kb on + strand, within DZA65_RS21035 at 4549.705 kb on + strand, within DZA65_RS21035 at 4549.706 kb on - strand, within DZA65_RS21035 at 4549.708 kb on + strand, within DZA65_RS21035 at 4549.736 kb on + strand, within DZA65_RS21035 at 4549.736 kb on + strand, within DZA65_RS21035 at 4549.736 kb on + strand, within DZA65_RS21035 at 4549.737 kb on - strand, within DZA65_RS21035 at 4549.737 kb on - strand, within DZA65_RS21035 at 4549.737 kb on - strand, within DZA65_RS21035 at 4549.737 kb on - strand, within DZA65_RS21035 at 4549.737 kb on - strand, within DZA65_RS21035 at 4549.791 kb on - strand, within DZA65_RS21035 at 4549.791 kb on - strand, within DZA65_RS21035 at 4549.851 kb on + strand, within DZA65_RS21035 at 4549.851 kb on + strand, within DZA65_RS21035 at 4549.851 kb on + strand, within DZA65_RS21035 at 4549.851 kb on + strand, within DZA65_RS21035 at 4549.852 kb on - strand, within DZA65_RS21035 at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.938 kb on + strand at 4549.939 kb on - strand at 4549.939 kb on - strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.948 kb on + strand at 4549.949 kb on - strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.950 kb on + strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4549.951 kb on - strand at 4550.008 kb on + strand at 4550.008 kb on + strand at 4550.009 kb on - strand at 4550.009 kb on - strand at 4550.014 kb on - strand at 4550.014 kb on - strand at 4550.025 kb on + strand at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.270 kb on + strand, within DZA65_RS21040 at 4550.271 kb on - strand, within DZA65_RS21040 at 4550.271 kb on - strand, within DZA65_RS21040 at 4550.271 kb on - strand, within DZA65_RS21040 at 4550.379 kb on + strand, within DZA65_RS21040 at 4550.380 kb on - strand at 4550.380 kb on - strand, within DZA65_RS21040 at 4550.423 kb on + strand, within DZA65_RS21040 at 4550.423 kb on + strand, within DZA65_RS21040 at 4550.423 kb on + strand, within DZA65_RS21040 at 4550.424 kb on - strand, within DZA65_RS21040 at 4550.424 kb on - strand, within DZA65_RS21040 at 4550.424 kb on - strand, within DZA65_RS21040 at 4550.424 kb on - strand, within DZA65_RS21040 at 4550.424 kb on - strand, within DZA65_RS21040 at 4550.476 kb on + strand, within DZA65_RS21040 at 4550.485 kb on + strand, within DZA65_RS21040 at 4550.485 kb on + strand, within DZA65_RS21040 at 4550.529 kb on + strand, within DZA65_RS21040 at 4550.530 kb on - strand, within DZA65_RS21040 at 4550.530 kb on - strand, within DZA65_RS21040 at 4550.711 kb on + strand at 4550.711 kb on + strand at 4550.712 kb on - strand at 4550.712 kb on - strand at 4550.712 kb on - strand at 4550.740 kb on + strand at 4550.740 kb on + strand at 4550.740 kb on + strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.741 kb on - strand at 4550.783 kb on + strand at 4550.783 kb on + strand at 4550.784 kb on - strand at 4550.895 kb on - strand at 4550.905 kb on + strand at 4550.905 kb on + strand at 4550.905 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,548,451 + DZA65_RS21030 0.28 +1.5 4,548,451 + DZA65_RS21030 0.28 +0.7 4,548,451 + DZA65_RS21030 0.28 +0.8 4,548,451 + DZA65_RS21030 0.28 +0.8 4,548,451 + DZA65_RS21030 0.28 -0.4 4,548,451 + DZA65_RS21030 0.28 +0.3 4,548,451 + DZA65_RS21030 0.28 +0.2 4,548,451 + DZA65_RS21030 0.28 +0.6 4,548,451 + DZA65_RS21030 0.28 +0.2 4,548,451 + DZA65_RS21030 0.28 -0.2 4,548,452 - DZA65_RS21030 0.28 -1.6 4,548,452 - DZA65_RS21030 0.28 -0.1 4,548,452 - DZA65_RS21030 0.28 -0.5 4,548,452 - DZA65_RS21030 0.28 -0.5 4,548,452 - DZA65_RS21030 0.28 +0.8 4,548,452 - DZA65_RS21030 0.28 +0.9 4,548,490 + DZA65_RS21030 0.32 +0.6 4,548,490 + DZA65_RS21030 0.32 -0.0 4,548,491 - DZA65_RS21030 0.32 -0.3 4,548,491 - DZA65_RS21030 0.32 -1.7 4,548,546 - DZA65_RS21030 0.38 -0.2 4,548,583 + DZA65_RS21030 0.41 -1.0 4,548,583 + DZA65_RS21030 0.41 +1.1 4,548,583 + DZA65_RS21030 0.41 +0.2 4,548,584 - DZA65_RS21030 0.42 -0.3 4,548,584 - DZA65_RS21030 0.42 -1.0 4,548,584 - DZA65_RS21030 0.42 -0.5 4,548,584 - DZA65_RS21030 0.42 -0.3 4,548,584 - DZA65_RS21030 0.42 +0.5 4,548,586 + DZA65_RS21030 0.42 +0.9 4,548,586 + DZA65_RS21030 0.42 +0.0 4,548,586 + DZA65_RS21030 0.42 +0.3 4,548,586 + DZA65_RS21030 0.42 -1.2 4,548,586 + DZA65_RS21030 0.42 -0.7 4,548,587 - DZA65_RS21030 0.42 -0.1 4,548,587 - DZA65_RS21030 0.42 +1.3 4,548,587 - DZA65_RS21030 0.42 -0.2 4,548,587 - DZA65_RS21030 0.42 +0.0 4,548,587 - DZA65_RS21030 0.42 -1.8 4,548,587 - DZA65_RS21030 0.42 +1.3 4,548,773 + DZA65_RS21030 0.61 -1.0 4,548,773 + DZA65_RS21030 0.61 +0.9 4,548,774 - DZA65_RS21030 0.61 -0.4 4,548,774 - DZA65_RS21030 0.61 +0.3 4,548,776 - DZA65_RS21030 0.61 +0.0 4,548,826 + DZA65_RS21030 0.66 -0.2 4,548,826 + DZA65_RS21030 0.66 -0.2 4,548,826 + DZA65_RS21030 0.66 -0.4 4,548,826 + DZA65_RS21030 0.66 +0.5 4,548,827 - DZA65_RS21030 0.66 +0.2 4,548,827 - DZA65_RS21030 0.66 +0.2 4,548,827 - DZA65_RS21030 0.66 -0.5 4,548,842 + DZA65_RS21030 0.68 -2.2 4,548,842 + DZA65_RS21030 0.68 +0.3 4,548,842 + DZA65_RS21030 0.68 +0.5 4,548,843 - DZA65_RS21030 0.68 +0.7 4,548,843 - DZA65_RS21030 0.68 -0.8 4,548,941 - DZA65_RS21030 0.78 -0.2 4,548,955 + DZA65_RS21030 0.79 +1.1 4,548,956 - DZA65_RS21030 0.79 -0.5 4,548,956 - DZA65_RS21030 0.79 +0.7 4,548,964 + DZA65_RS21030 0.80 -0.6 4,548,964 + DZA65_RS21030 0.80 +0.7 4,548,964 + DZA65_RS21030 0.80 +0.1 4,548,964 + DZA65_RS21030 0.80 +0.3 4,548,964 + DZA65_RS21030 0.80 -0.5 4,548,965 - DZA65_RS21030 0.80 +0.0 4,548,965 - DZA65_RS21030 0.80 +0.6 4,548,965 - DZA65_RS21030 0.80 +0.4 4,548,965 - DZA65_RS21030 0.80 -1.2 4,548,965 - DZA65_RS21030 0.80 -0.4 4,548,965 - DZA65_RS21030 0.80 -1.7 4,548,965 - +2.3 4,549,041 + DZA65_RS21030 0.88 -2.2 4,549,041 + DZA65_RS21030 0.88 +0.2 4,549,041 + DZA65_RS21030 0.88 +0.0 4,549,041 + DZA65_RS21030 0.88 -0.1 4,549,042 - DZA65_RS21030 0.88 +0.5 4,549,042 - DZA65_RS21030 0.88 -0.9 4,549,042 - DZA65_RS21030 0.88 -0.0 4,549,179 + -0.7 4,549,180 - -0.8 4,549,212 + +0.3 4,549,233 + -0.0 4,549,233 + -0.2 4,549,233 + +1.8 4,549,233 + -0.1 4,549,234 - -0.8 4,549,234 - -0.8 4,549,234 - +0.3 4,549,234 - -0.7 4,549,234 - +0.9 4,549,234 - -0.1 4,549,234 - +0.1 4,549,234 - +0.8 4,549,234 - -0.1 4,549,235 + -0.2 4,549,235 + +0.3 4,549,269 - -0.9 4,549,323 - -0.3 4,549,323 - -0.7 4,549,323 - +0.8 4,549,323 - -0.1 4,549,338 + +1.2 4,549,338 + +0.3 4,549,338 + +0.3 4,549,338 + -1.0 4,549,338 + +0.3 4,549,338 + -1.2 4,549,339 - +1.0 4,549,339 - +1.3 4,549,339 - -0.7 4,549,516 + DZA65_RS21035 0.35 -0.7 4,549,516 + DZA65_RS21035 0.35 +0.2 4,549,541 + DZA65_RS21035 0.39 +0.3 4,549,541 + DZA65_RS21035 0.39 -0.9 4,549,542 - DZA65_RS21035 0.39 +0.8 4,549,612 + DZA65_RS21035 0.51 -0.2 4,549,705 + DZA65_RS21035 0.66 +0.7 4,549,705 + DZA65_RS21035 0.66 +1.2 4,549,705 + DZA65_RS21035 0.66 -0.5 4,549,706 - DZA65_RS21035 0.66 -0.5 4,549,708 + DZA65_RS21035 0.66 +0.2 4,549,736 + DZA65_RS21035 0.71 +1.2 4,549,736 + DZA65_RS21035 0.71 +0.4 4,549,736 + DZA65_RS21035 0.71 +2.7 4,549,737 - DZA65_RS21035 0.71 -1.1 4,549,737 - DZA65_RS21035 0.71 +1.5 4,549,737 - DZA65_RS21035 0.71 +0.7 4,549,737 - DZA65_RS21035 0.71 +0.2 4,549,737 - DZA65_RS21035 0.71 -1.4 4,549,791 - DZA65_RS21035 0.79 -0.5 4,549,791 - DZA65_RS21035 0.79 +1.0 4,549,851 + DZA65_RS21035 0.89 +0.0 4,549,851 + DZA65_RS21035 0.89 +1.8 4,549,851 + DZA65_RS21035 0.89 +0.6 4,549,851 + DZA65_RS21035 0.89 -0.2 4,549,852 - DZA65_RS21035 0.89 -0.5 4,549,938 + +0.8 4,549,938 + +0.1 4,549,938 + +0.0 4,549,938 + -0.0 4,549,938 + +0.1 4,549,938 + -0.1 4,549,938 + +0.3 4,549,938 + -0.4 4,549,938 + -0.3 4,549,938 + -1.2 4,549,939 - +0.0 4,549,939 - +1.7 4,549,948 + -0.8 4,549,948 + -0.8 4,549,948 + +0.2 4,549,948 + -0.2 4,549,948 + -0.7 4,549,948 + -0.5 4,549,948 + -0.2 4,549,948 + +0.0 4,549,949 - -0.2 4,549,950 + +0.0 4,549,950 + +2.1 4,549,950 + -0.2 4,549,950 + -0.0 4,549,950 + -0.1 4,549,950 + -0.0 4,549,950 + +0.1 4,549,950 + +0.0 4,549,950 + -0.1 4,549,950 + +2.0 4,549,950 + +0.1 4,549,951 - +0.0 4,549,951 - -0.2 4,549,951 - +0.9 4,549,951 - +0.7 4,549,951 - +2.1 4,549,951 - +0.5 4,549,951 - -0.2 4,549,951 - -1.6 4,549,951 - -1.0 4,550,008 + -1.0 4,550,008 + +0.0 4,550,009 - +0.6 4,550,009 - +0.9 4,550,014 - +1.2 4,550,014 - -0.1 4,550,025 + +0.7 4,550,270 + DZA65_RS21040 0.37 +1.9 4,550,270 + DZA65_RS21040 0.37 -1.4 4,550,270 + DZA65_RS21040 0.37 -0.6 4,550,270 + DZA65_RS21040 0.37 +0.5 4,550,270 + DZA65_RS21040 0.37 -1.5 4,550,270 + DZA65_RS21040 0.37 +0.0 4,550,271 - DZA65_RS21040 0.37 -0.5 4,550,271 - DZA65_RS21040 0.37 -1.0 4,550,271 - DZA65_RS21040 0.37 +0.2 4,550,379 + DZA65_RS21040 0.54 -0.9 4,550,380 - +1.3 4,550,380 - DZA65_RS21040 0.54 +0.7 4,550,423 + DZA65_RS21040 0.61 -0.1 4,550,423 + DZA65_RS21040 0.61 -0.4 4,550,423 + DZA65_RS21040 0.61 -0.0 4,550,424 - DZA65_RS21040 0.61 +0.5 4,550,424 - DZA65_RS21040 0.61 -0.0 4,550,424 - DZA65_RS21040 0.61 +0.3 4,550,424 - DZA65_RS21040 0.61 +1.9 4,550,424 - DZA65_RS21040 0.61 -0.2 4,550,476 + DZA65_RS21040 0.70 -1.7 4,550,485 + DZA65_RS21040 0.71 +0.2 4,550,485 + DZA65_RS21040 0.71 +0.0 4,550,529 + DZA65_RS21040 0.78 -0.3 4,550,530 - DZA65_RS21040 0.78 +0.3 4,550,530 - DZA65_RS21040 0.78 +0.3 4,550,711 + +0.6 4,550,711 + +0.9 4,550,712 - +0.1 4,550,712 - -0.7 4,550,712 - +0.8 4,550,740 + +0.3 4,550,740 + -0.2 4,550,740 + +0.9 4,550,741 - -0.4 4,550,741 - +2.0 4,550,741 - +1.9 4,550,741 - +0.1 4,550,741 - +1.3 4,550,741 - +0.3 4,550,741 - +0.7 4,550,741 - +0.5 4,550,783 + +0.2 4,550,783 + +0.1 4,550,784 - -0.0 4,550,895 - +0.2 4,550,905 + +0.1 4,550,905 + -1.5 4,550,905 + +0.8
Or see this region's nucleotide sequence