Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05920 and DZA65_RS05925 overlap by 4 nucleotides DZA65_RS05925 and DZA65_RS05930 are separated by 241 nucleotides DZA65_RS05930 and DZA65_RS05935 are separated by 65 nucleotides DZA65_RS05935 and DZA65_RS05940 overlap by 38 nucleotides
DZA65_RS05920: DZA65_RS05920 - (2E,6E)-farnesyl diphosphate synthase, at 1,221,328 to 1,222,239
_RS05920
DZA65_RS05925: DZA65_RS05925 - exodeoxyribonuclease VII small subunit, at 1,222,236 to 1,222,484
_RS05925
DZA65_RS05930: DZA65_RS05930 - tRNA 4-thiouridine(8) synthase ThiI, at 1,222,726 to 1,224,174
_RS05930
DZA65_RS05935: DZA65_RS05935 - protein deglycase YajL, at 1,224,240 to 1,224,839
_RS05935
DZA65_RS05940: DZA65_RS05940 - 2-dehydropantoate 2-reductase, at 1,224,802 to 1,225,722
_RS05940
Position (kb)
1222
1223
1224
1225 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1222.506 kb on - strand at 1222.506 kb on - strand at 1222.506 kb on - strand at 1222.570 kb on - strand at 1222.570 kb on - strand at 1222.570 kb on - strand at 1222.578 kb on - strand at 1222.600 kb on - strand at 1222.608 kb on - strand at 1222.608 kb on - strand at 1222.608 kb on - strand at 1222.610 kb on - strand at 1222.610 kb on - strand at 1222.610 kb on - strand at 1222.610 kb on - strand at 1222.628 kb on + strand at 1222.629 kb on - strand at 1222.629 kb on - strand at 1222.671 kb on + strand at 1222.671 kb on + strand at 1222.671 kb on + strand at 1222.671 kb on + strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.672 kb on - strand at 1222.675 kb on + strand at 1222.683 kb on + strand at 1222.694 kb on + strand at 1222.695 kb on - strand at 1222.695 kb on - strand at 1222.740 kb on + strand at 1222.741 kb on - strand at 1222.741 kb on - strand at 1222.741 kb on - strand at 1222.792 kb on - strand at 1222.834 kb on + strand at 1222.872 kb on + strand, within DZA65_RS05930 at 1222.873 kb on - strand, within DZA65_RS05930 at 1222.972 kb on - strand, within DZA65_RS05930 at 1222.972 kb on - strand, within DZA65_RS05930 at 1222.972 kb on - strand, within DZA65_RS05930 at 1222.972 kb on - strand, within DZA65_RS05930 at 1222.975 kb on + strand, within DZA65_RS05930 at 1222.976 kb on - strand, within DZA65_RS05930 at 1222.992 kb on + strand, within DZA65_RS05930 at 1223.308 kb on + strand, within DZA65_RS05930 at 1223.309 kb on - strand, within DZA65_RS05930 at 1223.309 kb on - strand, within DZA65_RS05930 at 1223.309 kb on - strand, within DZA65_RS05930 at 1223.309 kb on - strand, within DZA65_RS05930 at 1223.341 kb on + strand, within DZA65_RS05930 at 1223.341 kb on + strand, within DZA65_RS05930 at 1223.341 kb on + strand, within DZA65_RS05930 at 1223.342 kb on - strand, within DZA65_RS05930 at 1223.342 kb on - strand, within DZA65_RS05930 at 1223.342 kb on - strand, within DZA65_RS05930 at 1223.342 kb on - strand, within DZA65_RS05930 at 1223.384 kb on + strand, within DZA65_RS05930 at 1223.385 kb on - strand, within DZA65_RS05930 at 1223.686 kb on - strand, within DZA65_RS05930 at 1224.034 kb on + strand at 1224.080 kb on - strand at 1224.127 kb on + strand at 1224.150 kb on + strand at 1224.164 kb on - strand at 1224.191 kb on + strand at 1224.191 kb on + strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.257 kb on - strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.269 kb on + strand at 1224.269 kb on + strand at 1224.270 kb on - strand at 1224.470 kb on + strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.487 kb on - strand, within DZA65_RS05935 at 1224.687 kb on + strand, within DZA65_RS05935 at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.859 kb on - strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.863 kb on + strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.927 kb on - strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.991 kb on - strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.050 kb on + strand, within DZA65_RS05940 at 1225.164 kb on + strand, within DZA65_RS05940 at 1225.164 kb on + strand, within DZA65_RS05940
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,222,506 - -0.5 1,222,506 - -0.0 1,222,506 - -0.6 1,222,570 - -0.3 1,222,570 - -1.0 1,222,570 - +0.6 1,222,578 - -2.3 1,222,600 - -0.2 1,222,608 - -0.8 1,222,608 - +0.2 1,222,608 - -0.0 1,222,610 - -0.1 1,222,610 - -0.0 1,222,610 - -0.2 1,222,610 - -0.6 1,222,628 + +1.0 1,222,629 - -1.5 1,222,629 - -0.0 1,222,671 + -1.3 1,222,671 + -0.4 1,222,671 + +0.4 1,222,671 + +0.5 1,222,672 - -0.6 1,222,672 - -1.6 1,222,672 - -1.9 1,222,672 - -1.4 1,222,672 - +1.0 1,222,672 - +0.7 1,222,672 - +0.3 1,222,675 + -0.0 1,222,683 + -1.1 1,222,694 + -0.8 1,222,695 - -1.6 1,222,695 - +1.0 1,222,740 + -0.3 1,222,741 - -2.2 1,222,741 - -1.8 1,222,741 - +1.3 1,222,792 - -1.2 1,222,834 + +0.4 1,222,872 + DZA65_RS05930 0.10 -0.2 1,222,873 - DZA65_RS05930 0.10 +1.5 1,222,972 - DZA65_RS05930 0.17 +0.6 1,222,972 - DZA65_RS05930 0.17 -2.5 1,222,972 - DZA65_RS05930 0.17 -2.3 1,222,972 - DZA65_RS05930 0.17 -2.0 1,222,975 + DZA65_RS05930 0.17 -1.0 1,222,976 - DZA65_RS05930 0.17 -2.5 1,222,992 + DZA65_RS05930 0.18 +0.5 1,223,308 + DZA65_RS05930 0.40 -2.6 1,223,309 - DZA65_RS05930 0.40 -0.0 1,223,309 - DZA65_RS05930 0.40 -0.2 1,223,309 - DZA65_RS05930 0.40 -0.3 1,223,309 - DZA65_RS05930 0.40 -1.6 1,223,341 + DZA65_RS05930 0.42 -1.6 1,223,341 + DZA65_RS05930 0.42 -1.7 1,223,341 + DZA65_RS05930 0.42 -0.6 1,223,342 - DZA65_RS05930 0.43 -1.1 1,223,342 - DZA65_RS05930 0.43 -2.2 1,223,342 - DZA65_RS05930 0.43 -0.3 1,223,342 - DZA65_RS05930 0.43 -0.5 1,223,384 + DZA65_RS05930 0.45 -1.2 1,223,385 - DZA65_RS05930 0.45 -1.7 1,223,686 - DZA65_RS05930 0.66 -0.4 1,224,034 + -1.8 1,224,080 - +0.2 1,224,127 + -0.0 1,224,150 + -0.3 1,224,164 - -0.8 1,224,191 + -0.3 1,224,191 + +0.1 1,224,192 - -1.1 1,224,192 - +0.7 1,224,192 - -0.8 1,224,192 - -0.2 1,224,257 - +1.1 1,224,259 + +0.6 1,224,259 + -0.3 1,224,259 + +0.4 1,224,259 + -0.3 1,224,259 + -0.2 1,224,260 - -0.6 1,224,260 - -0.3 1,224,260 - -0.3 1,224,260 - +1.0 1,224,260 - -1.0 1,224,260 - -0.0 1,224,260 - +0.1 1,224,260 - +0.1 1,224,260 - -2.3 1,224,265 + -0.2 1,224,265 + -0.6 1,224,265 + +1.8 1,224,265 + -0.4 1,224,265 + +0.6 1,224,265 + +0.3 1,224,265 + -0.0 1,224,266 - -1.3 1,224,266 - -0.6 1,224,266 - +0.4 1,224,266 - +0.2 1,224,266 - -0.0 1,224,266 - -0.0 1,224,266 - -0.8 1,224,269 + -1.5 1,224,269 + -0.3 1,224,270 - +1.7 1,224,470 + DZA65_RS05935 0.38 +0.2 1,224,471 - DZA65_RS05935 0.39 +0.2 1,224,471 - DZA65_RS05935 0.39 +0.7 1,224,471 - DZA65_RS05935 0.39 +0.2 1,224,487 - DZA65_RS05935 0.41 +1.1 1,224,687 + DZA65_RS05935 0.74 -0.0 1,224,839 + +0.2 1,224,839 + +0.8 1,224,859 - -0.9 1,224,861 + -2.2 1,224,861 + -2.7 1,224,861 + -0.6 1,224,861 + -0.3 1,224,861 + -2.3 1,224,862 - +0.1 1,224,862 - -0.3 1,224,862 - -0.4 1,224,862 - +1.0 1,224,862 - -1.0 1,224,862 - -0.2 1,224,862 - -0.3 1,224,862 - -1.7 1,224,863 + -0.6 1,224,864 - -0.8 1,224,864 - +0.3 1,224,864 - -0.6 1,224,864 - +0.4 1,224,927 - DZA65_RS05940 0.14 -0.0 1,224,936 + DZA65_RS05940 0.15 -0.8 1,224,936 + DZA65_RS05940 0.15 -0.8 1,224,936 + DZA65_RS05940 0.15 -0.6 1,224,936 + DZA65_RS05940 0.15 -0.2 1,224,936 + DZA65_RS05940 0.15 -0.5 1,224,936 + DZA65_RS05940 0.15 -1.8 1,224,937 - DZA65_RS05940 0.15 -0.2 1,224,937 - DZA65_RS05940 0.15 -1.6 1,224,937 - DZA65_RS05940 0.15 +0.5 1,224,937 - DZA65_RS05940 0.15 -0.4 1,224,937 - DZA65_RS05940 0.15 -1.0 1,224,938 + DZA65_RS05940 0.15 -0.1 1,224,938 + DZA65_RS05940 0.15 -0.2 1,224,938 + DZA65_RS05940 0.15 +0.6 1,224,938 + DZA65_RS05940 0.15 -0.4 1,224,938 + DZA65_RS05940 0.15 -0.1 1,224,938 + DZA65_RS05940 0.15 -0.9 1,224,938 + DZA65_RS05940 0.15 +1.0 1,224,938 + DZA65_RS05940 0.15 -0.6 1,224,938 + DZA65_RS05940 0.15 -0.6 1,224,939 - DZA65_RS05940 0.15 -1.0 1,224,939 - DZA65_RS05940 0.15 +0.7 1,224,939 - DZA65_RS05940 0.15 -0.1 1,224,939 - DZA65_RS05940 0.15 -1.3 1,224,991 - DZA65_RS05940 0.21 +0.7 1,225,025 + DZA65_RS05940 0.24 -0.6 1,225,025 + DZA65_RS05940 0.24 -0.2 1,225,025 + DZA65_RS05940 0.24 -0.8 1,225,025 + DZA65_RS05940 0.24 +0.7 1,225,025 + DZA65_RS05940 0.24 +0.3 1,225,025 + DZA65_RS05940 0.24 -1.0 1,225,025 + DZA65_RS05940 0.24 -0.6 1,225,025 + DZA65_RS05940 0.24 -0.6 1,225,026 - DZA65_RS05940 0.24 -1.0 1,225,026 - DZA65_RS05940 0.24 -0.0 1,225,026 - DZA65_RS05940 0.24 -0.7 1,225,026 - DZA65_RS05940 0.24 -0.6 1,225,026 - DZA65_RS05940 0.24 -0.4 1,225,026 - DZA65_RS05940 0.24 -0.8 1,225,026 - DZA65_RS05940 0.24 -1.3 1,225,026 - DZA65_RS05940 0.24 +1.0 1,225,026 - DZA65_RS05940 0.24 -1.2 1,225,026 - DZA65_RS05940 0.24 +0.2 1,225,050 + DZA65_RS05940 0.27 -0.8 1,225,164 + DZA65_RS05940 0.39 +0.1 1,225,164 + DZA65_RS05940 0.39 -0.8
Or see this region's nucleotide sequence