Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS04600 and DZA65_RS04605 are separated by 713 nucleotides DZA65_RS04605 and DZA65_RS04610 are separated by 111 nucleotides DZA65_RS04610 and DZA65_RS04615 are separated by 106 nucleotides
DZA65_RS04600: DZA65_RS04600 - conjugal transfer protein TraF, at 963,179 to 964,426
_RS04600
DZA65_RS04605: DZA65_RS04605 - helix-turn-helix transcriptional regulator, at 965,140 to 965,535
_RS04605
DZA65_RS04610: DZA65_RS04610 - transposase, at 965,647 to 965,808
_RS04610
DZA65_RS04615: DZA65_RS04615 - alpha/beta hydrolase, at 965,915 to 966,655
_RS04615
Position (kb) 965
966 Strain fitness (log2 ratio) -2
-1
0
1
2 at 964.142 kb on - strand, within DZA65_RS04600 at 964.181 kb on + strand, within DZA65_RS04600 at 964.181 kb on + strand, within DZA65_RS04600 at 964.181 kb on + strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.182 kb on - strand, within DZA65_RS04600 at 964.232 kb on - strand, within DZA65_RS04600 at 964.325 kb on - strand at 964.421 kb on - strand at 964.426 kb on + strand at 964.426 kb on + strand at 964.427 kb on - strand at 964.427 kb on - strand at 964.427 kb on - strand at 964.452 kb on + strand at 964.500 kb on + strand at 964.521 kb on - strand at 964.587 kb on - strand at 964.670 kb on - strand at 964.892 kb on - strand at 964.909 kb on + strand at 964.928 kb on - strand at 964.935 kb on + strand at 964.991 kb on + strand at 964.991 kb on + strand at 964.992 kb on - strand at 964.996 kb on - strand at 964.996 kb on - strand at 965.009 kb on + strand at 965.009 kb on + strand at 965.024 kb on - strand at 965.035 kb on - strand at 965.035 kb on - strand at 965.035 kb on - strand at 965.077 kb on - strand at 965.087 kb on + strand at 965.087 kb on + strand at 965.087 kb on + strand at 965.088 kb on - strand at 965.134 kb on + strand at 965.135 kb on - strand at 965.139 kb on + strand at 965.139 kb on + strand at 965.139 kb on + strand at 965.140 kb on - strand at 965.174 kb on + strand at 965.207 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.214 kb on + strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.215 kb on - strand, within DZA65_RS04605 at 965.313 kb on + strand, within DZA65_RS04605 at 965.313 kb on + strand, within DZA65_RS04605 at 965.313 kb on + strand, within DZA65_RS04605 at 965.313 kb on + strand, within DZA65_RS04605 at 965.313 kb on + strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.314 kb on - strand, within DZA65_RS04605 at 965.330 kb on + strand, within DZA65_RS04605 at 965.330 kb on + strand at 965.330 kb on + strand, within DZA65_RS04605 at 965.330 kb on + strand, within DZA65_RS04605 at 965.330 kb on + strand, within DZA65_RS04605 at 965.330 kb on + strand, within DZA65_RS04605 at 965.331 kb on - strand, within DZA65_RS04605 at 965.331 kb on - strand, within DZA65_RS04605 at 965.331 kb on - strand, within DZA65_RS04605 at 965.331 kb on - strand, within DZA65_RS04605 at 965.372 kb on - strand, within DZA65_RS04605 at 965.372 kb on - strand, within DZA65_RS04605 at 965.372 kb on - strand, within DZA65_RS04605 at 965.398 kb on + strand, within DZA65_RS04605 at 965.398 kb on + strand, within DZA65_RS04605 at 965.398 kb on + strand, within DZA65_RS04605 at 965.398 kb on + strand, within DZA65_RS04605 at 965.399 kb on - strand, within DZA65_RS04605 at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.543 kb on + strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.544 kb on - strand at 965.546 kb on - strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.608 kb on + strand at 965.609 kb on - strand at 965.609 kb on - strand at 965.609 kb on - strand at 965.609 kb on - strand at 965.609 kb on - strand at 965.615 kb on + strand at 965.615 kb on + strand at 965.616 kb on - strand at 965.633 kb on - strand at 965.795 kb on + strand at 965.796 kb on - strand at 965.814 kb on + strand at 965.825 kb on - strand at 965.825 kb on - strand at 965.855 kb on - strand at 965.908 kb on - strand at 965.954 kb on + strand at 965.955 kb on - strand at 965.985 kb on - strand at 966.013 kb on + strand, within DZA65_RS04615 at 966.079 kb on + strand, within DZA65_RS04615 at 966.085 kb on + strand, within DZA65_RS04615 at 966.241 kb on + strand, within DZA65_RS04615 at 966.312 kb on - strand, within DZA65_RS04615 at 966.358 kb on + strand, within DZA65_RS04615 at 966.358 kb on + strand, within DZA65_RS04615 at 966.359 kb on - strand, within DZA65_RS04615 at 966.361 kb on + strand, within DZA65_RS04615 at 966.371 kb on + strand, within DZA65_RS04615 at 966.411 kb on - strand, within DZA65_RS04615 at 966.434 kb on + strand, within DZA65_RS04615 at 966.469 kb on + strand, within DZA65_RS04615
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 964,142 - DZA65_RS04600 0.77 -0.3 964,181 + DZA65_RS04600 0.80 -0.4 964,181 + DZA65_RS04600 0.80 +0.6 964,181 + DZA65_RS04600 0.80 +0.3 964,182 - DZA65_RS04600 0.80 +1.1 964,182 - DZA65_RS04600 0.80 +1.1 964,182 - DZA65_RS04600 0.80 +0.1 964,182 - DZA65_RS04600 0.80 -0.7 964,182 - DZA65_RS04600 0.80 -1.1 964,182 - DZA65_RS04600 0.80 +0.1 964,232 - DZA65_RS04600 0.84 +0.1 964,325 - +0.4 964,421 - +0.1 964,426 + -0.1 964,426 + -0.8 964,427 - -0.1 964,427 - -0.1 964,427 - -0.0 964,452 + -0.6 964,500 + -1.0 964,521 - -0.1 964,587 - +0.3 964,670 - -0.5 964,892 - -2.4 964,909 + -0.7 964,928 - +1.4 964,935 + -1.3 964,991 + -0.1 964,991 + +0.2 964,992 - -0.9 964,996 - -0.0 964,996 - -0.9 965,009 + +0.1 965,009 + +0.5 965,024 - -0.5 965,035 - -0.5 965,035 - -0.2 965,035 - +0.4 965,077 - -0.1 965,087 + +0.9 965,087 + -0.0 965,087 + -0.0 965,088 - +0.9 965,134 + +0.1 965,135 - +1.4 965,139 + +0.2 965,139 + -0.1 965,139 + +1.1 965,140 - -0.2 965,174 + +0.5 965,207 + DZA65_RS04605 0.17 -0.9 965,214 + DZA65_RS04605 0.19 -0.2 965,214 + DZA65_RS04605 0.19 -0.1 965,214 + DZA65_RS04605 0.19 +1.1 965,214 + DZA65_RS04605 0.19 -0.9 965,214 + DZA65_RS04605 0.19 +0.3 965,214 + DZA65_RS04605 0.19 -0.3 965,214 + DZA65_RS04605 0.19 +0.8 965,215 - DZA65_RS04605 0.19 +0.1 965,215 - DZA65_RS04605 0.19 -0.5 965,215 - DZA65_RS04605 0.19 +0.0 965,215 - DZA65_RS04605 0.19 -0.7 965,215 - DZA65_RS04605 0.19 +0.0 965,215 - DZA65_RS04605 0.19 +0.6 965,215 - DZA65_RS04605 0.19 -0.3 965,313 + DZA65_RS04605 0.44 -0.2 965,313 + DZA65_RS04605 0.44 -0.3 965,313 + DZA65_RS04605 0.44 +0.3 965,313 + DZA65_RS04605 0.44 -0.4 965,313 + DZA65_RS04605 0.44 -0.4 965,314 - DZA65_RS04605 0.44 -2.2 965,314 - DZA65_RS04605 0.44 +0.4 965,314 - DZA65_RS04605 0.44 +0.8 965,314 - DZA65_RS04605 0.44 +0.3 965,314 - DZA65_RS04605 0.44 -2.0 965,314 - DZA65_RS04605 0.44 -0.7 965,314 - DZA65_RS04605 0.44 -0.3 965,330 + DZA65_RS04605 0.48 +0.7 965,330 + +0.8 965,330 + DZA65_RS04605 0.48 -0.3 965,330 + DZA65_RS04605 0.48 -1.4 965,330 + DZA65_RS04605 0.48 -1.7 965,330 + DZA65_RS04605 0.48 -0.1 965,331 - DZA65_RS04605 0.48 +0.3 965,331 - DZA65_RS04605 0.48 -1.7 965,331 - DZA65_RS04605 0.48 -0.1 965,331 - DZA65_RS04605 0.48 +1.4 965,372 - DZA65_RS04605 0.59 -0.9 965,372 - DZA65_RS04605 0.59 -1.0 965,372 - DZA65_RS04605 0.59 +0.3 965,398 + DZA65_RS04605 0.65 -0.5 965,398 + DZA65_RS04605 0.65 +0.1 965,398 + DZA65_RS04605 0.65 -2.1 965,398 + DZA65_RS04605 0.65 -0.8 965,399 - DZA65_RS04605 0.65 -1.6 965,543 + +0.6 965,543 + +0.2 965,543 + +0.1 965,543 + +1.1 965,543 + +1.7 965,543 + +0.2 965,543 + +0.3 965,543 + -1.8 965,543 + +0.1 965,543 + +1.1 965,544 - -0.7 965,544 - -0.9 965,544 - +0.3 965,544 - +1.4 965,544 - +0.3 965,544 - -0.9 965,544 - +0.1 965,544 - +0.4 965,544 - -0.1 965,544 - -0.7 965,544 - +1.1 965,544 - -0.9 965,546 - -0.0 965,608 + +0.6 965,608 + -0.7 965,608 + +2.1 965,608 + +1.1 965,608 + -0.4 965,608 + +0.3 965,608 + -1.1 965,608 + -0.4 965,608 + +0.2 965,609 - +0.1 965,609 - -1.1 965,609 - +0.9 965,609 - -0.2 965,609 - +1.6 965,615 + +0.1 965,615 + -0.1 965,616 - -0.8 965,633 - -0.9 965,795 + +0.9 965,796 - -0.6 965,814 + +0.1 965,825 - +0.0 965,825 - -0.5 965,855 - +0.1 965,908 - +0.5 965,954 + -0.1 965,955 - -0.9 965,985 - -0.4 966,013 + DZA65_RS04615 0.13 -0.1 966,079 + DZA65_RS04615 0.22 -0.2 966,085 + DZA65_RS04615 0.23 -0.4 966,241 + DZA65_RS04615 0.44 +0.9 966,312 - DZA65_RS04615 0.54 -0.2 966,358 + DZA65_RS04615 0.60 -0.1 966,358 + DZA65_RS04615 0.60 +2.6 966,359 - DZA65_RS04615 0.60 +0.9 966,361 + DZA65_RS04615 0.60 +0.1 966,371 + DZA65_RS04615 0.62 +0.3 966,411 - DZA65_RS04615 0.67 -0.4 966,434 + DZA65_RS04615 0.70 -0.1 966,469 + DZA65_RS04615 0.75 +0.1
Or see this region's nucleotide sequence