Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02465 and DZA65_RS02475 are separated by 338 nucleotides DZA65_RS02475 and DZA65_RS02480 are separated by 13 nucleotides DZA65_RS02480 and DZA65_RS02485 are separated by 71 nucleotides
DZA65_RS02465: DZA65_RS02465 - cell division protein ZapA, at 531,673 to 532,002
_RS02465
DZA65_RS02475: DZA65_RS02475 - 5-formyltetrahydrofolate cyclo-ligase, at 532,341 to 532,955
_RS02475
DZA65_RS02480: DZA65_RS02480 - multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR, at 532,969 to 534,225
_RS02480
DZA65_RS02485: DZA65_RS02485 - DNA repair protein RadA, at 534,297 to 535,682
_RS02485
Position (kb)
532
533
534
535 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 531.969 kb on + strand, within DZA65_RS02465 at 531.969 kb on + strand, within DZA65_RS02465 at 531.969 kb on + strand, within DZA65_RS02465 at 531.970 kb on - strand at 531.970 kb on - strand at 531.973 kb on - strand at 532.001 kb on - strand at 532.001 kb on - strand at 532.024 kb on + strand at 532.025 kb on - strand at 532.025 kb on - strand at 532.062 kb on + strand at 532.062 kb on + strand at 532.062 kb on + strand at 532.062 kb on + strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.063 kb on - strand at 532.128 kb on + strand at 532.129 kb on - strand at 532.148 kb on - strand at 532.157 kb on + strand at 532.158 kb on - strand at 532.194 kb on - strand at 532.239 kb on - strand at 532.239 kb on - strand at 532.277 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.310 kb on + strand at 532.311 kb on - strand at 532.311 kb on - strand at 532.311 kb on - strand at 532.319 kb on - strand at 532.387 kb on + strand at 532.388 kb on - strand at 532.596 kb on + strand, within DZA65_RS02475 at 532.596 kb on + strand, within DZA65_RS02475 at 532.596 kb on + strand, within DZA65_RS02475 at 532.642 kb on + strand, within DZA65_RS02475 at 532.642 kb on + strand, within DZA65_RS02475 at 532.642 kb on + strand, within DZA65_RS02475 at 532.643 kb on - strand, within DZA65_RS02475 at 532.807 kb on - strand, within DZA65_RS02475 at 532.807 kb on - strand, within DZA65_RS02475 at 532.958 kb on + strand at 532.958 kb on + strand at 532.971 kb on - strand at 533.054 kb on + strand at 533.054 kb on + strand at 533.078 kb on + strand at 533.078 kb on + strand at 533.078 kb on + strand at 533.078 kb on + strand at 533.078 kb on + strand at 533.079 kb on - strand at 533.079 kb on - strand at 533.079 kb on - strand at 533.079 kb on - strand at 533.079 kb on - strand at 533.083 kb on + strand at 533.083 kb on + strand at 533.084 kb on - strand at 533.084 kb on - strand at 533.084 kb on - strand at 533.103 kb on + strand, within DZA65_RS02480 at 533.103 kb on + strand, within DZA65_RS02480 at 533.103 kb on + strand, within DZA65_RS02480 at 533.103 kb on + strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.104 kb on - strand, within DZA65_RS02480 at 533.109 kb on + strand, within DZA65_RS02480 at 533.109 kb on + strand, within DZA65_RS02480 at 533.109 kb on + strand, within DZA65_RS02480 at 533.109 kb on + strand, within DZA65_RS02480 at 533.109 kb on + strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.110 kb on - strand, within DZA65_RS02480 at 533.262 kb on - strand, within DZA65_RS02480 at 533.426 kb on + strand at 533.426 kb on + strand, within DZA65_RS02480 at 533.426 kb on + strand, within DZA65_RS02480 at 533.426 kb on + strand, within DZA65_RS02480 at 533.426 kb on + strand, within DZA65_RS02480 at 533.426 kb on + strand, within DZA65_RS02480 at 533.427 kb on - strand, within DZA65_RS02480 at 533.427 kb on - strand, within DZA65_RS02480 at 533.427 kb on - strand, within DZA65_RS02480 at 533.427 kb on - strand, within DZA65_RS02480 at 533.439 kb on - strand, within DZA65_RS02480 at 533.439 kb on - strand, within DZA65_RS02480 at 533.467 kb on - strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.562 kb on + strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.563 kb on - strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.564 kb on + strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.565 kb on - strand, within DZA65_RS02480 at 533.574 kb on - strand, within DZA65_RS02480 at 533.616 kb on + strand at 533.616 kb on + strand, within DZA65_RS02480 at 533.617 kb on - strand, within DZA65_RS02480 at 533.617 kb on - strand, within DZA65_RS02480 at 533.617 kb on - strand, within DZA65_RS02480 at 533.669 kb on + strand, within DZA65_RS02480 at 533.670 kb on - strand, within DZA65_RS02480 at 533.670 kb on - strand, within DZA65_RS02480 at 533.670 kb on - strand, within DZA65_RS02480 at 533.670 kb on - strand, within DZA65_RS02480 at 533.699 kb on + strand, within DZA65_RS02480 at 533.700 kb on - strand, within DZA65_RS02480 at 533.829 kb on - strand, within DZA65_RS02480 at 533.905 kb on + strand, within DZA65_RS02480 at 533.906 kb on - strand, within DZA65_RS02480 at 533.906 kb on - strand, within DZA65_RS02480 at 533.953 kb on + strand, within DZA65_RS02480 at 533.954 kb on - strand, within DZA65_RS02480 at 533.954 kb on - strand, within DZA65_RS02480 at 533.981 kb on + strand, within DZA65_RS02480 at 533.981 kb on + strand, within DZA65_RS02480 at 533.982 kb on - strand, within DZA65_RS02480 at 533.982 kb on - strand, within DZA65_RS02480 at 534.130 kb on + strand at 534.130 kb on + strand at 534.130 kb on + strand at 534.130 kb on + strand at 534.130 kb on + strand at 534.130 kb on + strand at 534.131 kb on - strand at 534.131 kb on - strand at 534.131 kb on - strand at 534.131 kb on - strand at 534.134 kb on + strand at 534.148 kb on - strand at 534.209 kb on + strand at 534.209 kb on + strand at 534.209 kb on + strand at 534.210 kb on - strand at 534.215 kb on + strand at 534.215 kb on + strand at 534.215 kb on + strand at 534.216 kb on - strand at 534.233 kb on + strand at 534.234 kb on - strand at 534.263 kb on + strand at 534.263 kb on + strand at 534.263 kb on + strand at 534.263 kb on + strand at 534.263 kb on + strand at 534.263 kb on + strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.266 kb on + strand at 534.266 kb on + strand at 534.266 kb on + strand at 534.267 kb on - strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.281 kb on + strand at 534.282 kb on - strand at 534.282 kb on - strand at 534.282 kb on - strand at 534.282 kb on - strand at 534.282 kb on - strand at 534.282 kb on - strand at 534.298 kb on + strand at 534.298 kb on + strand at 534.381 kb on - strand at 534.381 kb on - strand at 534.663 kb on - strand, within DZA65_RS02485 at 534.874 kb on - strand, within DZA65_RS02485 at 534.971 kb on + strand, within DZA65_RS02485 at 534.971 kb on + strand, within DZA65_RS02485 at 534.971 kb on + strand, within DZA65_RS02485 at 534.972 kb on - strand, within DZA65_RS02485 at 534.972 kb on - strand at 535.072 kb on + strand, within DZA65_RS02485 at 535.073 kb on - strand, within DZA65_RS02485
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 531,969 + DZA65_RS02465 0.90 -0.1 531,969 + DZA65_RS02465 0.90 +0.1 531,969 + DZA65_RS02465 0.90 +0.3 531,970 - -0.2 531,970 - +0.2 531,973 - -0.2 532,001 - -1.3 532,001 - +0.8 532,024 + +0.8 532,025 - +0.8 532,025 - +2.2 532,062 + +1.1 532,062 + +0.1 532,062 + +0.1 532,062 + -0.2 532,063 - +1.0 532,063 - +0.6 532,063 - +0.0 532,063 - -1.8 532,063 - -0.9 532,063 - +0.6 532,063 - +0.3 532,063 - +0.4 532,128 + +0.5 532,129 - +0.9 532,148 - +1.4 532,157 + +0.8 532,158 - +0.1 532,194 - +1.0 532,239 - +0.6 532,239 - +1.6 532,277 + -0.7 532,297 + -0.2 532,297 + +0.5 532,297 + +0.1 532,297 + -0.2 532,297 + -0.9 532,310 + +0.5 532,311 - +1.0 532,311 - +0.7 532,311 - -1.9 532,319 - +1.0 532,387 + +0.1 532,388 - +0.2 532,596 + DZA65_RS02475 0.41 -0.2 532,596 + DZA65_RS02475 0.41 +0.2 532,596 + DZA65_RS02475 0.41 -0.1 532,642 + DZA65_RS02475 0.49 -0.0 532,642 + DZA65_RS02475 0.49 +0.5 532,642 + DZA65_RS02475 0.49 -1.6 532,643 - DZA65_RS02475 0.49 -1.1 532,807 - DZA65_RS02475 0.76 -0.6 532,807 - DZA65_RS02475 0.76 -2.2 532,958 + -1.4 532,958 + -0.1 532,971 - -0.5 533,054 + -0.4 533,054 + +0.5 533,078 + +1.5 533,078 + -1.4 533,078 + +1.3 533,078 + +1.0 533,078 + +0.7 533,079 - +1.1 533,079 - +1.0 533,079 - +0.1 533,079 - -0.3 533,079 - -0.8 533,083 + -0.8 533,083 + +0.1 533,084 - -0.4 533,084 - +1.8 533,084 - +0.3 533,103 + DZA65_RS02480 0.11 -0.0 533,103 + DZA65_RS02480 0.11 +0.2 533,103 + DZA65_RS02480 0.11 +1.0 533,103 + DZA65_RS02480 0.11 +1.0 533,104 - DZA65_RS02480 0.11 +1.0 533,104 - DZA65_RS02480 0.11 +2.6 533,104 - DZA65_RS02480 0.11 +1.0 533,104 - DZA65_RS02480 0.11 -1.8 533,104 - DZA65_RS02480 0.11 +0.8 533,104 - DZA65_RS02480 0.11 +1.0 533,109 + DZA65_RS02480 0.11 +0.5 533,109 + DZA65_RS02480 0.11 +0.1 533,109 + DZA65_RS02480 0.11 -0.2 533,109 + DZA65_RS02480 0.11 +2.0 533,109 + DZA65_RS02480 0.11 +1.2 533,110 - DZA65_RS02480 0.11 +0.1 533,110 - DZA65_RS02480 0.11 +0.4 533,110 - DZA65_RS02480 0.11 +0.9 533,110 - DZA65_RS02480 0.11 +0.3 533,110 - DZA65_RS02480 0.11 -1.1 533,110 - DZA65_RS02480 0.11 +0.3 533,110 - DZA65_RS02480 0.11 +2.0 533,262 - DZA65_RS02480 0.23 +0.0 533,426 + +2.2 533,426 + DZA65_RS02480 0.36 -0.4 533,426 + DZA65_RS02480 0.36 +0.5 533,426 + DZA65_RS02480 0.36 +0.6 533,426 + DZA65_RS02480 0.36 -0.8 533,426 + DZA65_RS02480 0.36 +1.2 533,427 - DZA65_RS02480 0.36 +0.9 533,427 - DZA65_RS02480 0.36 -0.1 533,427 - DZA65_RS02480 0.36 +0.5 533,427 - DZA65_RS02480 0.36 +0.1 533,439 - DZA65_RS02480 0.37 +2.0 533,439 - DZA65_RS02480 0.37 -0.1 533,467 - DZA65_RS02480 0.40 +1.0 533,562 + DZA65_RS02480 0.47 +1.0 533,562 + DZA65_RS02480 0.47 +0.6 533,562 + DZA65_RS02480 0.47 +0.6 533,562 + DZA65_RS02480 0.47 -1.1 533,562 + DZA65_RS02480 0.47 -0.4 533,562 + DZA65_RS02480 0.47 +1.5 533,562 + DZA65_RS02480 0.47 -0.6 533,562 + DZA65_RS02480 0.47 +0.2 533,562 + DZA65_RS02480 0.47 -0.4 533,563 - DZA65_RS02480 0.47 +2.5 533,563 - +0.8 533,563 - DZA65_RS02480 0.47 +0.4 533,563 - DZA65_RS02480 0.47 +0.8 533,563 - DZA65_RS02480 0.47 -1.2 533,563 - DZA65_RS02480 0.47 +0.7 533,563 - DZA65_RS02480 0.47 +0.8 533,563 - DZA65_RS02480 0.47 +0.3 533,563 - DZA65_RS02480 0.47 +0.2 533,563 - DZA65_RS02480 0.47 +0.7 533,564 + DZA65_RS02480 0.47 +1.2 533,564 + DZA65_RS02480 0.47 +0.1 533,564 + DZA65_RS02480 0.47 +0.2 533,564 + DZA65_RS02480 0.47 +0.6 533,564 + DZA65_RS02480 0.47 -0.5 533,564 + DZA65_RS02480 0.47 +1.5 533,565 - DZA65_RS02480 0.47 -0.4 533,565 - DZA65_RS02480 0.47 +0.7 533,565 - DZA65_RS02480 0.47 -0.1 533,565 - DZA65_RS02480 0.47 +0.7 533,565 - DZA65_RS02480 0.47 -0.1 533,565 - DZA65_RS02480 0.47 +0.4 533,565 - DZA65_RS02480 0.47 -1.1 533,565 - DZA65_RS02480 0.47 -0.3 533,574 - DZA65_RS02480 0.48 +1.2 533,616 + +0.2 533,616 + DZA65_RS02480 0.51 -0.6 533,617 - DZA65_RS02480 0.52 -0.1 533,617 - DZA65_RS02480 0.52 +0.8 533,617 - DZA65_RS02480 0.52 +0.9 533,669 + DZA65_RS02480 0.56 +1.6 533,670 - DZA65_RS02480 0.56 -0.2 533,670 - DZA65_RS02480 0.56 -0.2 533,670 - DZA65_RS02480 0.56 +0.1 533,670 - DZA65_RS02480 0.56 +1.0 533,699 + DZA65_RS02480 0.58 +0.8 533,700 - DZA65_RS02480 0.58 +0.9 533,829 - DZA65_RS02480 0.68 -0.2 533,905 + DZA65_RS02480 0.74 -0.1 533,906 - DZA65_RS02480 0.75 +0.6 533,906 - DZA65_RS02480 0.75 +0.0 533,953 + DZA65_RS02480 0.78 -0.6 533,954 - DZA65_RS02480 0.78 +1.0 533,954 - DZA65_RS02480 0.78 -0.0 533,981 + DZA65_RS02480 0.81 +1.0 533,981 + DZA65_RS02480 0.81 +0.0 533,982 - DZA65_RS02480 0.81 -0.7 533,982 - DZA65_RS02480 0.81 +0.0 534,130 + -1.1 534,130 + +1.5 534,130 + +0.1 534,130 + -0.4 534,130 + -0.4 534,130 + +0.4 534,131 - +0.6 534,131 - -1.1 534,131 - +0.2 534,131 - +1.5 534,134 + +1.0 534,148 - +0.1 534,209 + +0.6 534,209 + -2.1 534,209 + +0.8 534,210 - +0.1 534,215 + -0.6 534,215 + +1.7 534,215 + +2.2 534,216 - +0.7 534,233 + -0.4 534,234 - +1.5 534,263 + +0.2 534,263 + +0.4 534,263 + +0.9 534,263 + +0.6 534,263 + +0.8 534,263 + +2.0 534,264 - +0.8 534,264 - +0.1 534,264 - +1.0 534,264 - +0.9 534,264 - -1.2 534,264 - +1.5 534,264 - -0.5 534,266 + -0.8 534,266 + +1.4 534,266 + -0.2 534,267 - -0.2 534,281 + -0.8 534,281 + +2.2 534,281 + +1.2 534,281 + +0.8 534,281 + +0.3 534,281 + -0.1 534,281 + -1.0 534,282 - +1.0 534,282 - -1.3 534,282 - -1.8 534,282 - +0.1 534,282 - -0.0 534,282 - +0.1 534,298 + +0.6 534,298 + -1.8 534,381 - +0.8 534,381 - +0.2 534,663 - DZA65_RS02485 0.26 -0.3 534,874 - DZA65_RS02485 0.42 -0.4 534,971 + DZA65_RS02485 0.49 +0.9 534,971 + DZA65_RS02485 0.49 +0.5 534,971 + DZA65_RS02485 0.49 -1.1 534,972 - DZA65_RS02485 0.49 -0.9 534,972 - -0.8 535,072 + DZA65_RS02485 0.56 -0.4 535,073 - DZA65_RS02485 0.56 -0.3
Or see this region's nucleotide sequence