Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01265 and DZA65_RS01270 are separated by 82 nucleotides DZA65_RS01270 and DZA65_RS01275 are separated by 185 nucleotides DZA65_RS01275 and DZA65_RS01280 are separated by 102 nucleotides DZA65_RS01280 and DZA65_RS01285 are separated by 280 nucleotides
DZA65_RS01265: DZA65_RS01265 - 16S ribosomal RNA, at 282,076 to 283,623
_RS01265
DZA65_RS01270: DZA65_RS01270 - tRNA-Glu, at 283,706 to 283,781
_RS01270
DZA65_RS01275: DZA65_RS01275 - 23S ribosomal RNA, at 283,967 to 286,990
_RS01275
DZA65_RS01280: DZA65_RS01280 - 5S ribosomal RNA, at 287,093 to 287,208
_RS01280
DZA65_RS01285: DZA65_RS01285 - amino acid ABC transporter ATP-binding protein, at 287,489 to 288,250
_RS01285
Position (kb) 283
284
285
286
287 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 285.328 kb on + strand, within DZA65_RS01275 at 286.144 kb on + strand, within DZA65_RS01275 at 287.323 kb on - strand at 287.458 kb on - strand at 287.458 kb on - strand at 287.458 kb on - strand at 287.491 kb on - strand at 287.499 kb on - strand at 287.499 kb on - strand at 287.521 kb on + strand at 287.521 kb on + strand at 287.521 kb on + strand at 287.522 kb on - strand at 287.522 kb on - strand at 287.522 kb on - strand at 287.591 kb on - strand, within DZA65_RS01285 at 287.591 kb on - strand, within DZA65_RS01285 at 287.591 kb on - strand, within DZA65_RS01285 at 287.614 kb on + strand, within DZA65_RS01285 at 287.821 kb on + strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.822 kb on - strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.856 kb on + strand, within DZA65_RS01285 at 287.857 kb on - strand, within DZA65_RS01285 at 287.857 kb on - strand, within DZA65_RS01285 at 287.920 kb on + strand, within DZA65_RS01285 at 287.920 kb on + strand, within DZA65_RS01285 at 287.921 kb on - strand, within DZA65_RS01285 at 287.921 kb on - strand, within DZA65_RS01285
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 285,328 + DZA65_RS01275 0.45 -1.7 285,328 + DZA65_RS01275 0.45 -1.1 285,328 + DZA65_RS01275 0.45 -1.1 285,328 + DZA65_RS01275 0.45 -1.0 285,328 + DZA65_RS01275 0.45 -1.1 286,144 + DZA65_RS01275 0.72 +0.2 287,323 - -0.6 287,458 - +1.2 287,458 - -1.8 287,458 - -0.0 287,491 - -1.1 287,499 - +0.3 287,499 - +0.7 287,521 + -0.7 287,521 + +0.1 287,521 + -1.3 287,522 - -3.3 287,522 - +0.4 287,522 - -0.6 287,591 - DZA65_RS01285 0.13 +0.1 287,591 - DZA65_RS01285 0.13 -1.8 287,591 - DZA65_RS01285 0.13 -0.6 287,614 + DZA65_RS01285 0.16 +0.7 287,821 + DZA65_RS01285 0.44 +0.6 287,822 - DZA65_RS01285 0.44 -0.1 287,822 - DZA65_RS01285 0.44 -0.1 287,822 - DZA65_RS01285 0.44 -2.0 287,822 - DZA65_RS01285 0.44 +0.6 287,856 + DZA65_RS01285 0.48 -0.7 287,856 + DZA65_RS01285 0.48 -0.2 287,856 + DZA65_RS01285 0.48 -0.1 287,857 - DZA65_RS01285 0.48 +0.5 287,857 - DZA65_RS01285 0.48 +1.0 287,920 + DZA65_RS01285 0.57 -0.7 287,920 + DZA65_RS01285 0.57 +0.8 287,921 - DZA65_RS01285 0.57 -0.2 287,921 - DZA65_RS01285 0.57 -0.2
Or see this region's nucleotide sequence