Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01195 and DZA65_RS01200 are separated by 1 nucleotides DZA65_RS01200 and DZA65_RS01205 overlap by 4 nucleotides DZA65_RS01205 and DZA65_RS01210 are separated by 1 nucleotides DZA65_RS01210 and DZA65_RS01215 overlap by 1 nucleotides
DZA65_RS01195: DZA65_RS01195 - thiazole synthase, at 268,704 to 269,483
_RS01195
DZA65_RS01200: DZA65_RS01200 - sulfur carrier protein ThiS, at 269,485 to 269,685
_RS01200
DZA65_RS01205: DZA65_RS01205 - HesA/MoeB/ThiF family protein, at 269,682 to 270,431
_RS01205
DZA65_RS01210: DZA65_RS01210 - thiamine phosphate synthase, at 270,433 to 271,068
_RS01210
DZA65_RS01215: DZA65_RS01215 - phosphomethylpyrimidine synthase ThiC, at 271,068 to 273,035
_RS01215
Position (kb) 270
271
272 Strain fitness (log2 ratio) -2
-1
0
1
2 at 269.456 kb on + strand at 269.465 kb on + strand at 269.920 kb on + strand, within DZA65_RS01205 at 269.920 kb on + strand, within DZA65_RS01205 at 270.146 kb on + strand, within DZA65_RS01205 at 270.292 kb on + strand, within DZA65_RS01205 at 270.293 kb on - strand, within DZA65_RS01205 at 270.293 kb on - strand, within DZA65_RS01205 at 270.410 kb on + strand at 270.410 kb on + strand at 270.410 kb on + strand at 270.410 kb on + strand at 270.411 kb on - strand at 270.411 kb on - strand at 270.411 kb on - strand at 270.411 kb on - strand at 270.434 kb on + strand at 270.435 kb on - strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.455 kb on - strand at 270.455 kb on - strand at 270.455 kb on - strand at 270.464 kb on - strand at 270.681 kb on + strand, within DZA65_RS01210 at 270.696 kb on + strand, within DZA65_RS01210 at 270.696 kb on + strand, within DZA65_RS01210 at 270.696 kb on + strand, within DZA65_RS01210 at 270.697 kb on - strand, within DZA65_RS01210 at 270.697 kb on - strand, within DZA65_RS01210 at 270.697 kb on - strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.698 kb on + strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.699 kb on - strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.823 kb on + strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.824 kb on - strand, within DZA65_RS01210 at 270.831 kb on - strand, within DZA65_RS01210 at 270.860 kb on + strand, within DZA65_RS01210 at 270.888 kb on - strand, within DZA65_RS01210 at 270.951 kb on + strand, within DZA65_RS01210 at 270.952 kb on - strand, within DZA65_RS01210 at 270.958 kb on + strand, within DZA65_RS01210 at 270.959 kb on - strand, within DZA65_RS01210 at 270.959 kb on - strand, within DZA65_RS01210 at 270.959 kb on - strand, within DZA65_RS01210 at 270.970 kb on - strand, within DZA65_RS01210 at 271.026 kb on - strand at 271.105 kb on + strand at 271.105 kb on + strand at 271.105 kb on + strand at 271.105 kb on + strand at 271.105 kb on + strand at 271.105 kb on + strand at 271.106 kb on - strand at 271.334 kb on + strand, within DZA65_RS01215 at 271.335 kb on - strand, within DZA65_RS01215 at 271.335 kb on - strand, within DZA65_RS01215 at 271.335 kb on - strand, within DZA65_RS01215 at 271.444 kb on + strand, within DZA65_RS01215 at 271.444 kb on + strand, within DZA65_RS01215 at 271.444 kb on + strand, within DZA65_RS01215 at 271.462 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.510 kb on + strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.511 kb on - strand, within DZA65_RS01215 at 271.608 kb on + strand, within DZA65_RS01215 at 271.608 kb on + strand, within DZA65_RS01215 at 271.608 kb on + strand, within DZA65_RS01215 at 271.609 kb on - strand, within DZA65_RS01215 at 271.609 kb on - strand, within DZA65_RS01215 at 271.615 kb on + strand, within DZA65_RS01215 at 271.615 kb on + strand, within DZA65_RS01215 at 271.616 kb on - strand, within DZA65_RS01215 at 271.616 kb on - strand, within DZA65_RS01215 at 271.616 kb on - strand, within DZA65_RS01215 at 271.616 kb on - strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.710 kb on + strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.711 kb on - strand, within DZA65_RS01215 at 271.717 kb on + strand, within DZA65_RS01215 at 271.718 kb on - strand, within DZA65_RS01215 at 271.718 kb on - strand, within DZA65_RS01215 at 271.718 kb on - strand, within DZA65_RS01215 at 271.837 kb on + strand, within DZA65_RS01215 at 271.837 kb on + strand, within DZA65_RS01215 at 271.838 kb on - strand, within DZA65_RS01215 at 271.876 kb on + strand, within DZA65_RS01215 at 271.876 kb on + strand, within DZA65_RS01215 at 271.877 kb on - strand, within DZA65_RS01215 at 271.877 kb on - strand, within DZA65_RS01215 at 271.975 kb on + strand, within DZA65_RS01215 at 271.975 kb on + strand, within DZA65_RS01215 at 271.975 kb on + strand, within DZA65_RS01215 at 271.976 kb on - strand, within DZA65_RS01215 at 271.976 kb on - strand, within DZA65_RS01215 at 271.976 kb on - strand, within DZA65_RS01215 at 271.976 kb on - strand, within DZA65_RS01215 at 272.008 kb on + strand, within DZA65_RS01215 at 272.008 kb on + strand, within DZA65_RS01215 at 272.008 kb on + strand, within DZA65_RS01215 at 272.009 kb on - strand, within DZA65_RS01215 at 272.009 kb on - strand, within DZA65_RS01215
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 269,456 + -1.8 269,465 + -1.6 269,920 + DZA65_RS01205 0.32 +1.1 269,920 + DZA65_RS01205 0.32 +0.1 270,146 + DZA65_RS01205 0.62 -1.0 270,292 + DZA65_RS01205 0.81 +0.3 270,293 - DZA65_RS01205 0.81 +0.0 270,293 - DZA65_RS01205 0.81 -1.7 270,410 + +1.1 270,410 + -1.1 270,410 + -1.3 270,410 + +0.2 270,411 - -0.7 270,411 - -0.5 270,411 - +0.2 270,411 - +0.2 270,434 + -1.6 270,435 - -1.7 270,454 + -0.9 270,454 + -0.2 270,454 + -0.1 270,454 + -1.3 270,455 - +0.4 270,455 - +0.5 270,455 - -0.9 270,464 - -0.9 270,681 + DZA65_RS01210 0.39 -0.4 270,696 + DZA65_RS01210 0.41 +1.6 270,696 + DZA65_RS01210 0.41 -1.0 270,696 + DZA65_RS01210 0.41 +0.6 270,697 - DZA65_RS01210 0.42 -0.4 270,697 - DZA65_RS01210 0.42 -0.4 270,697 - DZA65_RS01210 0.42 -2.3 270,698 + DZA65_RS01210 0.42 -0.5 270,698 + DZA65_RS01210 0.42 -1.5 270,698 + DZA65_RS01210 0.42 -0.6 270,698 + DZA65_RS01210 0.42 -0.4 270,698 + DZA65_RS01210 0.42 -0.5 270,698 + DZA65_RS01210 0.42 +0.7 270,698 + DZA65_RS01210 0.42 -1.0 270,698 + DZA65_RS01210 0.42 -1.4 270,698 + DZA65_RS01210 0.42 -1.6 270,698 + DZA65_RS01210 0.42 -0.1 270,698 + DZA65_RS01210 0.42 -0.8 270,698 + DZA65_RS01210 0.42 -0.9 270,698 + DZA65_RS01210 0.42 -1.8 270,698 + DZA65_RS01210 0.42 -1.4 270,699 - DZA65_RS01210 0.42 +0.7 270,699 - DZA65_RS01210 0.42 +0.3 270,699 - DZA65_RS01210 0.42 +0.5 270,699 - DZA65_RS01210 0.42 -1.1 270,699 - DZA65_RS01210 0.42 +0.0 270,699 - DZA65_RS01210 0.42 +0.0 270,699 - DZA65_RS01210 0.42 -0.3 270,699 - DZA65_RS01210 0.42 -0.7 270,699 - DZA65_RS01210 0.42 -0.7 270,699 - DZA65_RS01210 0.42 -1.5 270,699 - DZA65_RS01210 0.42 -0.6 270,699 - DZA65_RS01210 0.42 -1.1 270,699 - DZA65_RS01210 0.42 -1.6 270,823 + DZA65_RS01210 0.61 -1.3 270,823 + DZA65_RS01210 0.61 -0.7 270,823 + DZA65_RS01210 0.61 -0.7 270,823 + DZA65_RS01210 0.61 -0.5 270,823 + DZA65_RS01210 0.61 -0.3 270,823 + DZA65_RS01210 0.61 -2.0 270,823 + DZA65_RS01210 0.61 -1.2 270,823 + DZA65_RS01210 0.61 -1.7 270,824 - DZA65_RS01210 0.61 -1.4 270,824 - DZA65_RS01210 0.61 +1.0 270,824 - DZA65_RS01210 0.61 +0.0 270,824 - DZA65_RS01210 0.61 -1.1 270,824 - DZA65_RS01210 0.61 -2.1 270,824 - DZA65_RS01210 0.61 -0.5 270,824 - DZA65_RS01210 0.61 -0.8 270,824 - DZA65_RS01210 0.61 -0.8 270,831 - DZA65_RS01210 0.63 -1.1 270,860 + DZA65_RS01210 0.67 -0.7 270,888 - DZA65_RS01210 0.72 -0.5 270,951 + DZA65_RS01210 0.81 -0.2 270,952 - DZA65_RS01210 0.82 -0.8 270,958 + DZA65_RS01210 0.83 -1.6 270,959 - DZA65_RS01210 0.83 -0.8 270,959 - DZA65_RS01210 0.83 -0.7 270,959 - DZA65_RS01210 0.83 -0.9 270,970 - DZA65_RS01210 0.84 -2.1 271,026 - -0.5 271,105 + -1.5 271,105 + -1.8 271,105 + -0.1 271,105 + -0.7 271,105 + -0.9 271,105 + -1.3 271,106 - -0.8 271,334 + DZA65_RS01215 0.14 -0.3 271,335 - DZA65_RS01215 0.14 -0.3 271,335 - DZA65_RS01215 0.14 -1.3 271,335 - DZA65_RS01215 0.14 -0.9 271,444 + DZA65_RS01215 0.19 -1.5 271,444 + DZA65_RS01215 0.19 -1.9 271,444 + DZA65_RS01215 0.19 -1.8 271,462 + DZA65_RS01215 0.20 +0.6 271,510 + DZA65_RS01215 0.22 -0.5 271,510 + DZA65_RS01215 0.22 -1.3 271,510 + DZA65_RS01215 0.22 -1.0 271,510 + DZA65_RS01215 0.22 +1.1 271,510 + DZA65_RS01215 0.22 -0.6 271,510 + DZA65_RS01215 0.22 -2.4 271,510 + DZA65_RS01215 0.22 -0.1 271,510 + DZA65_RS01215 0.22 -0.1 271,510 + DZA65_RS01215 0.22 -1.2 271,510 + DZA65_RS01215 0.22 -0.8 271,511 - DZA65_RS01215 0.23 -2.1 271,511 - DZA65_RS01215 0.23 -0.9 271,511 - DZA65_RS01215 0.23 -1.3 271,511 - DZA65_RS01215 0.23 -0.4 271,511 - DZA65_RS01215 0.23 +0.5 271,511 - DZA65_RS01215 0.23 -0.7 271,511 - DZA65_RS01215 0.23 -0.4 271,511 - DZA65_RS01215 0.23 -0.8 271,608 + DZA65_RS01215 0.27 +0.6 271,608 + DZA65_RS01215 0.27 +0.3 271,608 + DZA65_RS01215 0.27 -0.8 271,609 - DZA65_RS01215 0.27 -2.1 271,609 - DZA65_RS01215 0.27 -1.5 271,615 + DZA65_RS01215 0.28 +0.0 271,615 + DZA65_RS01215 0.28 -1.3 271,616 - DZA65_RS01215 0.28 -0.9 271,616 - DZA65_RS01215 0.28 +0.4 271,616 - DZA65_RS01215 0.28 +0.3 271,616 - DZA65_RS01215 0.28 +0.0 271,710 + DZA65_RS01215 0.33 -0.7 271,710 + DZA65_RS01215 0.33 -1.0 271,710 + DZA65_RS01215 0.33 -0.8 271,710 + DZA65_RS01215 0.33 -0.5 271,710 + DZA65_RS01215 0.33 -0.7 271,710 + DZA65_RS01215 0.33 -1.6 271,710 + DZA65_RS01215 0.33 -1.0 271,710 + DZA65_RS01215 0.33 -0.1 271,710 + DZA65_RS01215 0.33 -1.9 271,710 + DZA65_RS01215 0.33 -1.1 271,710 + DZA65_RS01215 0.33 -1.6 271,710 + DZA65_RS01215 0.33 -0.6 271,710 + DZA65_RS01215 0.33 -0.2 271,711 - DZA65_RS01215 0.33 -1.9 271,711 - DZA65_RS01215 0.33 -0.9 271,711 - DZA65_RS01215 0.33 -0.8 271,711 - DZA65_RS01215 0.33 +1.3 271,711 - DZA65_RS01215 0.33 -1.6 271,711 - DZA65_RS01215 0.33 +0.2 271,711 - DZA65_RS01215 0.33 +2.1 271,711 - DZA65_RS01215 0.33 -1.2 271,711 - DZA65_RS01215 0.33 -2.1 271,711 - DZA65_RS01215 0.33 -1.6 271,711 - DZA65_RS01215 0.33 -1.8 271,711 - DZA65_RS01215 0.33 +1.0 271,711 - DZA65_RS01215 0.33 -0.9 271,717 + DZA65_RS01215 0.33 -0.3 271,718 - DZA65_RS01215 0.33 +1.3 271,718 - DZA65_RS01215 0.33 -1.6 271,718 - DZA65_RS01215 0.33 -1.9 271,837 + DZA65_RS01215 0.39 -0.8 271,837 + DZA65_RS01215 0.39 -1.3 271,838 - DZA65_RS01215 0.39 -1.3 271,876 + DZA65_RS01215 0.41 +0.0 271,876 + DZA65_RS01215 0.41 -1.5 271,877 - DZA65_RS01215 0.41 -0.8 271,877 - DZA65_RS01215 0.41 -0.8 271,975 + DZA65_RS01215 0.46 -1.5 271,975 + DZA65_RS01215 0.46 -1.6 271,975 + DZA65_RS01215 0.46 -0.5 271,976 - DZA65_RS01215 0.46 +0.0 271,976 - DZA65_RS01215 0.46 -0.6 271,976 - DZA65_RS01215 0.46 -1.5 271,976 - DZA65_RS01215 0.46 -1.3 272,008 + DZA65_RS01215 0.48 -1.5 272,008 + DZA65_RS01215 0.48 +0.2 272,008 + DZA65_RS01215 0.48 -0.1 272,009 - DZA65_RS01215 0.48 -0.9 272,009 - DZA65_RS01215 0.48 -1.4
Or see this region's nucleotide sequence