Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00935 and DZA65_RS00940 are separated by 24 nucleotides DZA65_RS00940 and DZA65_RS00945 are separated by 116 nucleotides
DZA65_RS00935: DZA65_RS00935 - N-acetyl-gamma-glutamyl-phosphate reductase, at 214,532 to 215,536
_RS00935
DZA65_RS00940: DZA65_RS00940 - acetylglutamate kinase, at 215,561 to 216,334
_RS00940
DZA65_RS00945: DZA65_RS00945 - argininosuccinate lyase, at 216,451 to 217,824
_RS00945
Position (kb) 215
216
217 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 214.565 kb on + strand at 214.565 kb on + strand at 214.565 kb on + strand at 214.566 kb on - strand at 214.567 kb on + strand at 214.567 kb on + strand at 214.567 kb on + strand at 214.567 kb on + strand at 214.567 kb on + strand at 214.567 kb on + strand at 214.568 kb on - strand at 214.568 kb on - strand at 214.589 kb on + strand at 214.589 kb on + strand at 214.589 kb on + strand at 214.590 kb on - strand at 214.590 kb on - strand at 214.590 kb on - strand at 214.617 kb on + strand at 214.617 kb on + strand at 214.618 kb on - strand at 214.618 kb on - strand at 214.627 kb on - strand at 214.687 kb on + strand, within DZA65_RS00935 at 214.687 kb on + strand, within DZA65_RS00935 at 214.687 kb on + strand, within DZA65_RS00935 at 214.687 kb on + strand, within DZA65_RS00935 at 214.688 kb on - strand, within DZA65_RS00935 at 214.688 kb on - strand, within DZA65_RS00935 at 214.704 kb on + strand, within DZA65_RS00935 at 214.822 kb on + strand, within DZA65_RS00935 at 214.822 kb on + strand, within DZA65_RS00935 at 214.822 kb on + strand, within DZA65_RS00935 at 214.822 kb on + strand, within DZA65_RS00935 at 214.823 kb on - strand, within DZA65_RS00935 at 214.823 kb on - strand, within DZA65_RS00935 at 214.872 kb on - strand, within DZA65_RS00935 at 214.872 kb on - strand, within DZA65_RS00935 at 214.874 kb on - strand, within DZA65_RS00935 at 214.874 kb on - strand, within DZA65_RS00935 at 214.880 kb on + strand, within DZA65_RS00935 at 214.880 kb on + strand, within DZA65_RS00935 at 214.883 kb on + strand, within DZA65_RS00935 at 214.883 kb on + strand, within DZA65_RS00935 at 214.884 kb on - strand, within DZA65_RS00935 at 214.903 kb on + strand, within DZA65_RS00935 at 214.928 kb on + strand, within DZA65_RS00935 at 214.928 kb on + strand, within DZA65_RS00935 at 214.928 kb on + strand, within DZA65_RS00935 at 214.928 kb on + strand, within DZA65_RS00935 at 214.929 kb on - strand, within DZA65_RS00935 at 214.929 kb on - strand, within DZA65_RS00935 at 214.929 kb on - strand, within DZA65_RS00935 at 214.957 kb on + strand, within DZA65_RS00935 at 214.957 kb on + strand, within DZA65_RS00935 at 214.957 kb on + strand, within DZA65_RS00935 at 214.957 kb on + strand, within DZA65_RS00935 at 214.958 kb on - strand, within DZA65_RS00935 at 214.958 kb on - strand, within DZA65_RS00935 at 214.976 kb on - strand, within DZA65_RS00935 at 215.245 kb on + strand, within DZA65_RS00935 at 215.245 kb on + strand, within DZA65_RS00935 at 215.245 kb on + strand, within DZA65_RS00935 at 215.246 kb on - strand, within DZA65_RS00935 at 215.246 kb on - strand, within DZA65_RS00935 at 215.246 kb on - strand, within DZA65_RS00935 at 215.260 kb on + strand, within DZA65_RS00935 at 215.260 kb on + strand, within DZA65_RS00935 at 215.260 kb on + strand, within DZA65_RS00935 at 215.261 kb on - strand, within DZA65_RS00935 at 215.261 kb on - strand, within DZA65_RS00935 at 215.310 kb on - strand, within DZA65_RS00935 at 215.310 kb on - strand, within DZA65_RS00935 at 215.321 kb on + strand, within DZA65_RS00935 at 215.321 kb on + strand, within DZA65_RS00935 at 215.322 kb on - strand, within DZA65_RS00935 at 215.349 kb on - strand, within DZA65_RS00935 at 215.349 kb on - strand, within DZA65_RS00935 at 215.349 kb on - strand, within DZA65_RS00935 at 215.349 kb on - strand, within DZA65_RS00935 at 215.349 kb on - strand, within DZA65_RS00935 at 215.452 kb on + strand at 215.452 kb on + strand at 215.452 kb on + strand at 215.453 kb on - strand at 215.453 kb on - strand at 215.453 kb on - strand at 215.484 kb on + strand at 215.485 kb on - strand at 215.485 kb on - strand at 215.529 kb on + strand at 215.529 kb on + strand at 215.529 kb on + strand at 215.530 kb on - strand at 215.530 kb on - strand at 215.571 kb on + strand at 215.571 kb on + strand at 215.572 kb on - strand at 215.572 kb on - strand at 215.575 kb on + strand at 215.575 kb on + strand at 215.575 kb on + strand at 215.576 kb on - strand at 215.583 kb on + strand at 215.583 kb on + strand at 215.583 kb on + strand at 215.583 kb on + strand at 215.584 kb on - strand at 215.584 kb on - strand at 215.584 kb on - strand at 215.584 kb on - strand at 215.592 kb on + strand at 215.592 kb on + strand at 215.592 kb on + strand at 215.593 kb on - strand at 215.593 kb on - strand at 215.593 kb on - strand at 215.593 kb on - strand at 215.595 kb on + strand at 215.595 kb on + strand at 215.595 kb on + strand at 215.596 kb on - strand at 215.782 kb on + strand, within DZA65_RS00940 at 215.782 kb on + strand, within DZA65_RS00940 at 215.782 kb on + strand, within DZA65_RS00940 at 215.782 kb on + strand, within DZA65_RS00940 at 215.782 kb on + strand, within DZA65_RS00940 at 215.783 kb on - strand, within DZA65_RS00940 at 215.783 kb on - strand, within DZA65_RS00940 at 215.786 kb on - strand, within DZA65_RS00940 at 215.786 kb on - strand, within DZA65_RS00940 at 215.892 kb on + strand, within DZA65_RS00940 at 215.979 kb on - strand, within DZA65_RS00940 at 216.154 kb on + strand, within DZA65_RS00940 at 216.181 kb on + strand, within DZA65_RS00940 at 216.181 kb on + strand, within DZA65_RS00940 at 216.182 kb on - strand, within DZA65_RS00940 at 216.184 kb on + strand, within DZA65_RS00940 at 216.184 kb on + strand, within DZA65_RS00940 at 216.184 kb on + strand, within DZA65_RS00940 at 216.184 kb on + strand, within DZA65_RS00940 at 216.185 kb on - strand, within DZA65_RS00940 at 216.185 kb on - strand, within DZA65_RS00940 at 216.185 kb on - strand, within DZA65_RS00940 at 216.185 kb on - strand, within DZA65_RS00940 at 216.185 kb on - strand, within DZA65_RS00940 at 216.197 kb on - strand, within DZA65_RS00940 at 216.205 kb on + strand, within DZA65_RS00940 at 216.285 kb on + strand at 216.310 kb on + strand at 216.332 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.350 kb on + strand at 216.351 kb on - strand at 216.351 kb on - strand at 216.351 kb on - strand at 216.351 kb on - strand at 216.351 kb on - strand at 216.351 kb on - strand at 216.368 kb on + strand at 216.368 kb on + strand at 216.369 kb on - strand at 216.369 kb on - strand at 216.426 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.446 kb on + strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.447 kb on - strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.450 kb on + strand at 216.451 kb on - strand at 216.451 kb on - strand at 216.451 kb on - strand at 216.451 kb on - strand at 216.451 kb on - strand at 216.451 kb on - strand at 216.489 kb on + strand at 216.489 kb on + strand at 216.490 kb on - strand at 216.499 kb on - strand at 216.499 kb on - strand at 216.529 kb on + strand at 216.561 kb on + strand at 216.573 kb on + strand at 216.573 kb on + strand at 216.573 kb on + strand at 216.574 kb on - strand at 216.574 kb on - strand at 216.574 kb on - strand at 216.590 kb on + strand, within DZA65_RS00945 at 216.591 kb on - strand, within DZA65_RS00945 at 216.591 kb on - strand, within DZA65_RS00945 at 216.591 kb on - strand, within DZA65_RS00945 at 216.596 kb on + strand, within DZA65_RS00945 at 216.597 kb on - strand, within DZA65_RS00945 at 216.597 kb on - strand, within DZA65_RS00945 at 216.597 kb on - strand, within DZA65_RS00945 at 216.597 kb on - strand, within DZA65_RS00945 at 216.675 kb on + strand, within DZA65_RS00945 at 216.675 kb on + strand, within DZA65_RS00945 at 216.675 kb on + strand, within DZA65_RS00945 at 216.676 kb on - strand, within DZA65_RS00945 at 216.676 kb on - strand, within DZA65_RS00945 at 216.676 kb on - strand, within DZA65_RS00945 at 216.676 kb on - strand, within DZA65_RS00945 at 216.676 kb on - strand, within DZA65_RS00945 at 216.714 kb on - strand, within DZA65_RS00945 at 216.910 kb on + strand, within DZA65_RS00945 at 216.910 kb on + strand, within DZA65_RS00945 at 216.911 kb on - strand, within DZA65_RS00945 at 216.911 kb on - strand, within DZA65_RS00945 at 216.912 kb on + strand, within DZA65_RS00945 at 216.912 kb on + strand, within DZA65_RS00945 at 216.913 kb on - strand, within DZA65_RS00945 at 216.965 kb on - strand, within DZA65_RS00945 at 216.965 kb on - strand, within DZA65_RS00945 at 217.005 kb on + strand, within DZA65_RS00945 at 217.005 kb on + strand, within DZA65_RS00945 at 217.005 kb on + strand, within DZA65_RS00945 at 217.005 kb on + strand at 217.005 kb on + strand, within DZA65_RS00945 at 217.005 kb on + strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.006 kb on - strand, within DZA65_RS00945 at 217.069 kb on - strand, within DZA65_RS00945 at 217.180 kb on - strand, within DZA65_RS00945 at 217.185 kb on + strand, within DZA65_RS00945 at 217.269 kb on + strand, within DZA65_RS00945 at 217.269 kb on + strand, within DZA65_RS00945 at 217.269 kb on + strand, within DZA65_RS00945 at 217.269 kb on + strand, within DZA65_RS00945 at 217.270 kb on - strand, within DZA65_RS00945
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 214,565 + -1.9 214,565 + -2.4 214,565 + -3.4 214,566 - -0.6 214,567 + -1.4 214,567 + -0.4 214,567 + -2.7 214,567 + -2.6 214,567 + +0.5 214,567 + -2.0 214,568 - -1.0 214,568 - -0.4 214,589 + -3.3 214,589 + -2.1 214,589 + -2.1 214,590 - -1.0 214,590 - -2.4 214,590 - -1.2 214,617 + -2.3 214,617 + -1.0 214,618 - -0.6 214,618 - -3.5 214,627 - -2.3 214,687 + DZA65_RS00935 0.15 -1.2 214,687 + DZA65_RS00935 0.15 -0.8 214,687 + DZA65_RS00935 0.15 -0.4 214,687 + DZA65_RS00935 0.15 -3.1 214,688 - DZA65_RS00935 0.16 -1.5 214,688 - DZA65_RS00935 0.16 -1.5 214,704 + DZA65_RS00935 0.17 -0.2 214,822 + DZA65_RS00935 0.29 -1.3 214,822 + DZA65_RS00935 0.29 -0.7 214,822 + DZA65_RS00935 0.29 -2.2 214,822 + DZA65_RS00935 0.29 -0.6 214,823 - DZA65_RS00935 0.29 -3.2 214,823 - DZA65_RS00935 0.29 -3.4 214,872 - DZA65_RS00935 0.34 -2.0 214,872 - DZA65_RS00935 0.34 -1.6 214,874 - DZA65_RS00935 0.34 +0.1 214,874 - DZA65_RS00935 0.34 -1.6 214,880 + DZA65_RS00935 0.35 +1.4 214,880 + DZA65_RS00935 0.35 +0.4 214,883 + DZA65_RS00935 0.35 -0.3 214,883 + DZA65_RS00935 0.35 -2.7 214,884 - DZA65_RS00935 0.35 -0.8 214,903 + DZA65_RS00935 0.37 -2.2 214,928 + DZA65_RS00935 0.39 +0.4 214,928 + DZA65_RS00935 0.39 -0.0 214,928 + DZA65_RS00935 0.39 -2.4 214,928 + DZA65_RS00935 0.39 -2.1 214,929 - DZA65_RS00935 0.40 -0.6 214,929 - DZA65_RS00935 0.40 -2.1 214,929 - DZA65_RS00935 0.40 -1.6 214,957 + DZA65_RS00935 0.42 -2.0 214,957 + DZA65_RS00935 0.42 -2.5 214,957 + DZA65_RS00935 0.42 +0.4 214,957 + DZA65_RS00935 0.42 -0.4 214,958 - DZA65_RS00935 0.42 -1.2 214,958 - DZA65_RS00935 0.42 -2.5 214,976 - DZA65_RS00935 0.44 -1.2 215,245 + DZA65_RS00935 0.71 -3.5 215,245 + DZA65_RS00935 0.71 -1.2 215,245 + DZA65_RS00935 0.71 -1.4 215,246 - DZA65_RS00935 0.71 -2.2 215,246 - DZA65_RS00935 0.71 -0.6 215,246 - DZA65_RS00935 0.71 -0.6 215,260 + DZA65_RS00935 0.72 -3.4 215,260 + DZA65_RS00935 0.72 +0.0 215,260 + DZA65_RS00935 0.72 -2.1 215,261 - DZA65_RS00935 0.73 -2.4 215,261 - DZA65_RS00935 0.73 -0.3 215,310 - DZA65_RS00935 0.77 -1.1 215,310 - DZA65_RS00935 0.77 -1.0 215,321 + DZA65_RS00935 0.79 -2.8 215,321 + DZA65_RS00935 0.79 -1.9 215,322 - DZA65_RS00935 0.79 +0.6 215,349 - DZA65_RS00935 0.81 -2.0 215,349 - DZA65_RS00935 0.81 -0.1 215,349 - DZA65_RS00935 0.81 -2.8 215,349 - DZA65_RS00935 0.81 -1.2 215,349 - DZA65_RS00935 0.81 -2.0 215,452 + -1.0 215,452 + -2.0 215,452 + -2.3 215,453 - -3.2 215,453 - -2.1 215,453 - -3.0 215,484 + -2.2 215,485 - -0.4 215,485 - -1.5 215,529 + +1.4 215,529 + +0.4 215,529 + +0.1 215,530 - -3.3 215,530 - -1.2 215,571 + -1.6 215,571 + -2.2 215,572 - -1.4 215,572 - -1.2 215,575 + -4.7 215,575 + -0.6 215,575 + -2.2 215,576 - -1.0 215,583 + -2.1 215,583 + -1.0 215,583 + -3.1 215,583 + -1.4 215,584 - -0.6 215,584 - -2.1 215,584 - -1.8 215,584 - -2.2 215,592 + -2.1 215,592 + +0.1 215,592 + -0.6 215,593 - -2.6 215,593 - -2.8 215,593 - -1.6 215,593 - -3.8 215,595 + -3.4 215,595 + -3.5 215,595 + -0.6 215,596 - -0.0 215,782 + DZA65_RS00940 0.29 -0.6 215,782 + DZA65_RS00940 0.29 -1.8 215,782 + DZA65_RS00940 0.29 -3.1 215,782 + DZA65_RS00940 0.29 -1.3 215,782 + DZA65_RS00940 0.29 -1.0 215,783 - DZA65_RS00940 0.29 -1.0 215,783 - DZA65_RS00940 0.29 -2.2 215,786 - DZA65_RS00940 0.29 -3.8 215,786 - DZA65_RS00940 0.29 -3.5 215,892 + DZA65_RS00940 0.43 -1.0 215,979 - DZA65_RS00940 0.54 -2.3 216,154 + DZA65_RS00940 0.77 +0.0 216,181 + DZA65_RS00940 0.80 -1.0 216,181 + DZA65_RS00940 0.80 -1.7 216,182 - DZA65_RS00940 0.80 -1.9 216,184 + DZA65_RS00940 0.80 -3.2 216,184 + DZA65_RS00940 0.80 -2.8 216,184 + DZA65_RS00940 0.80 -3.0 216,184 + DZA65_RS00940 0.80 -2.9 216,185 - DZA65_RS00940 0.81 -1.2 216,185 - DZA65_RS00940 0.81 -1.2 216,185 - DZA65_RS00940 0.81 -2.5 216,185 - DZA65_RS00940 0.81 +0.2 216,185 - DZA65_RS00940 0.81 -3.8 216,197 - DZA65_RS00940 0.82 -1.2 216,205 + DZA65_RS00940 0.83 -2.4 216,285 + +0.5 216,310 + -0.0 216,332 + -1.0 216,350 + +0.8 216,350 + -0.1 216,350 + -0.4 216,350 + -0.7 216,350 + -1.8 216,350 + -0.1 216,350 + -0.4 216,350 + +0.2 216,350 + +0.4 216,351 - +0.4 216,351 - -1.6 216,351 - -1.1 216,351 - -1.8 216,351 - -2.2 216,351 - -0.2 216,368 + +1.7 216,368 + +0.8 216,369 - -1.8 216,369 - -1.3 216,426 + -0.6 216,446 + -3.3 216,446 + -2.0 216,446 + -2.5 216,446 + -1.4 216,446 + -2.0 216,446 + -3.7 216,446 + -2.3 216,446 + -3.1 216,446 + -0.0 216,446 + +0.6 216,446 + -2.7 216,446 + -1.1 216,446 + -2.9 216,446 + -2.8 216,446 + +0.1 216,446 + -2.1 216,446 + -2.8 216,446 + -1.2 216,446 + -2.6 216,447 - -2.4 216,447 - -2.7 216,447 - -2.7 216,447 - -0.6 216,447 - -1.4 216,447 - -3.2 216,447 - -2.3 216,447 - -1.2 216,447 - -1.4 216,447 - -3.7 216,447 - -3.3 216,450 + -1.8 216,450 + -1.2 216,450 + +0.4 216,450 + -2.0 216,451 - -4.0 216,451 - -0.2 216,451 - -1.6 216,451 - -2.5 216,451 - -0.6 216,451 - +0.1 216,489 + -2.1 216,489 + +0.8 216,490 - -1.2 216,499 - -1.2 216,499 - +0.4 216,529 + -3.0 216,561 + -0.4 216,573 + -1.2 216,573 + -1.9 216,573 + -3.2 216,574 - -3.1 216,574 - +1.4 216,574 - -3.6 216,590 + DZA65_RS00945 0.10 -1.3 216,591 - DZA65_RS00945 0.10 -1.4 216,591 - DZA65_RS00945 0.10 -1.5 216,591 - DZA65_RS00945 0.10 -1.2 216,596 + DZA65_RS00945 0.11 -1.4 216,597 - DZA65_RS00945 0.11 -2.1 216,597 - DZA65_RS00945 0.11 -2.0 216,597 - DZA65_RS00945 0.11 -1.8 216,597 - DZA65_RS00945 0.11 -1.2 216,675 + DZA65_RS00945 0.16 -2.7 216,675 + DZA65_RS00945 0.16 -3.2 216,675 + DZA65_RS00945 0.16 -1.3 216,676 - DZA65_RS00945 0.16 -2.0 216,676 - DZA65_RS00945 0.16 -2.8 216,676 - DZA65_RS00945 0.16 -3.2 216,676 - DZA65_RS00945 0.16 +0.2 216,676 - DZA65_RS00945 0.16 -3.7 216,714 - DZA65_RS00945 0.19 -1.9 216,910 + DZA65_RS00945 0.33 -2.9 216,910 + DZA65_RS00945 0.33 +0.7 216,911 - DZA65_RS00945 0.33 -1.4 216,911 - DZA65_RS00945 0.33 -2.1 216,912 + DZA65_RS00945 0.34 -2.9 216,912 + DZA65_RS00945 0.34 -2.8 216,913 - DZA65_RS00945 0.34 -1.0 216,965 - DZA65_RS00945 0.37 -2.9 216,965 - DZA65_RS00945 0.37 -0.2 217,005 + DZA65_RS00945 0.40 -2.8 217,005 + DZA65_RS00945 0.40 -1.4 217,005 + DZA65_RS00945 0.40 -2.5 217,005 + +1.4 217,005 + DZA65_RS00945 0.40 -1.2 217,005 + DZA65_RS00945 0.40 -0.4 217,006 - DZA65_RS00945 0.40 -0.2 217,006 - DZA65_RS00945 0.40 +0.0 217,006 - DZA65_RS00945 0.40 -1.0 217,006 - DZA65_RS00945 0.40 -2.9 217,006 - DZA65_RS00945 0.40 -1.2 217,006 - DZA65_RS00945 0.40 -1.6 217,006 - DZA65_RS00945 0.40 -0.9 217,069 - DZA65_RS00945 0.45 -2.2 217,180 - DZA65_RS00945 0.53 -1.0 217,185 + DZA65_RS00945 0.53 -1.6 217,269 + DZA65_RS00945 0.60 -1.2 217,269 + DZA65_RS00945 0.60 -1.5 217,269 + DZA65_RS00945 0.60 -2.1 217,269 + DZA65_RS00945 0.60 -1.2 217,270 - DZA65_RS00945 0.60 -2.0
Or see this region's nucleotide sequence