Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19145 and DZA65_RS19150 overlap by 14 nucleotides DZA65_RS19150 and DZA65_RS19155 are separated by 19 nucleotides DZA65_RS19155 and DZA65_RS19160 overlap by 4 nucleotides
DZA65_RS19145: DZA65_RS19145 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase, at 4,139,190 to 4,140,674
_RS19145
DZA65_RS19150: DZA65_RS19150 - peptidoglycan glycosyltransferase FtsI, at 4,140,661 to 4,142,424
_RS19150
DZA65_RS19155: DZA65_RS19155 - cell division protein FtsL, at 4,142,444 to 4,142,767
_RS19155
DZA65_RS19160: DZA65_RS19160 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 4,142,764 to 4,143,705
_RS19160
Position (kb)
4140
4141
4142
4143 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4140.698 kb on - strand at 4140.742 kb on - strand at 4140.742 kb on - strand at 4140.742 kb on - strand at 4142.441 kb on - strand at 4143.073 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.075 kb on - strand, within DZA65_RS19160 at 4143.117 kb on - strand, within DZA65_RS19160 at 4143.325 kb on - strand, within DZA65_RS19160
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,140,698 - -0.2 4,140,742 - +3.3 4,140,742 - +1.2 4,140,742 - +0.1 4,142,441 - +0.3 4,143,073 - DZA65_RS19160 0.33 +0.4 4,143,075 - DZA65_RS19160 0.33 +1.1 4,143,075 - DZA65_RS19160 0.33 -1.1 4,143,075 - DZA65_RS19160 0.33 -0.1 4,143,075 - DZA65_RS19160 0.33 +0.1 4,143,075 - DZA65_RS19160 0.33 +1.1 4,143,075 - DZA65_RS19160 0.33 -0.2 4,143,117 - DZA65_RS19160 0.37 +1.1 4,143,325 - DZA65_RS19160 0.60 -0.1
Or see this region's nucleotide sequence