Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10675 and DZA65_RS10680 are separated by 152 nucleotides DZA65_RS10680 and DZA65_RS10685 are separated by 267 nucleotides DZA65_RS10685 and DZA65_RS10690 are separated by 139 nucleotides DZA65_RS10690 and DZA65_RS22875 are separated by 223 nucleotides
DZA65_RS10675: DZA65_RS10675 - hypothetical protein, at 2,262,676 to 2,263,077
_RS10675
DZA65_RS10680: DZA65_RS10680 - phage holin, lambda family, at 2,263,230 to 2,263,355
_RS10680
DZA65_RS10685: DZA65_RS10685 - hypothetical protein, at 2,263,623 to 2,264,228
_RS10685
DZA65_RS10690: DZA65_RS10690 - hypothetical protein, at 2,264,368 to 2,264,601
_RS10690
DZA65_RS22875: DZA65_RS22875 - hypothetical protein, at 2,264,825 to 2,264,983
_RS22875
Position (kb)
2263
2264
2265 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2262.652 kb on + strand at 2262.653 kb on - strand at 2262.673 kb on - strand at 2262.673 kb on - strand at 2262.675 kb on + strand at 2262.718 kb on + strand, within DZA65_RS10675 at 2262.719 kb on - strand, within DZA65_RS10675 at 2262.719 kb on - strand, within DZA65_RS10675 at 2262.719 kb on - strand, within DZA65_RS10675 at 2262.730 kb on - strand, within DZA65_RS10675 at 2262.769 kb on - strand, within DZA65_RS10675 at 2262.784 kb on - strand at 2262.847 kb on + strand, within DZA65_RS10675 at 2262.847 kb on + strand, within DZA65_RS10675 at 2262.847 kb on + strand, within DZA65_RS10675 at 2262.847 kb on + strand, within DZA65_RS10675 at 2262.848 kb on - strand, within DZA65_RS10675 at 2262.875 kb on - strand, within DZA65_RS10675 at 2262.878 kb on + strand, within DZA65_RS10675 at 2262.904 kb on + strand, within DZA65_RS10675 at 2262.905 kb on - strand, within DZA65_RS10675 at 2263.059 kb on - strand at 2263.081 kb on + strand at 2263.081 kb on + strand at 2263.081 kb on + strand at 2263.082 kb on - strand at 2263.082 kb on - strand at 2263.082 kb on - strand at 2263.083 kb on + strand at 2263.083 kb on + strand at 2263.083 kb on + strand at 2263.083 kb on + strand at 2263.120 kb on + strand at 2263.120 kb on + strand at 2263.121 kb on - strand at 2263.121 kb on - strand at 2263.190 kb on + strand at 2263.191 kb on - strand at 2263.197 kb on + strand at 2263.198 kb on - strand at 2263.293 kb on + strand, within DZA65_RS10680 at 2263.302 kb on - strand, within DZA65_RS10680 at 2263.302 kb on - strand, within DZA65_RS10680 at 2263.302 kb on - strand, within DZA65_RS10680 at 2263.334 kb on + strand, within DZA65_RS10680 at 2263.335 kb on - strand, within DZA65_RS10680 at 2263.335 kb on - strand, within DZA65_RS10680 at 2263.338 kb on - strand, within DZA65_RS10680 at 2263.357 kb on - strand at 2263.401 kb on + strand at 2263.425 kb on + strand at 2263.425 kb on + strand at 2263.426 kb on - strand at 2263.426 kb on - strand at 2263.524 kb on + strand at 2263.529 kb on + strand at 2263.529 kb on + strand at 2263.529 kb on + strand at 2263.530 kb on - strand at 2263.541 kb on + strand at 2263.545 kb on + strand at 2263.552 kb on + strand at 2263.553 kb on - strand at 2263.553 kb on - strand at 2263.587 kb on + strand at 2263.588 kb on - strand at 2263.588 kb on - strand at 2263.588 kb on - strand at 2263.591 kb on + strand at 2263.618 kb on + strand at 2263.622 kb on + strand at 2263.709 kb on + strand, within DZA65_RS10685 at 2263.792 kb on - strand, within DZA65_RS10685 at 2263.794 kb on - strand, within DZA65_RS10685 at 2263.794 kb on - strand, within DZA65_RS10685 at 2263.809 kb on + strand, within DZA65_RS10685 at 2263.828 kb on + strand, within DZA65_RS10685 at 2263.843 kb on + strand, within DZA65_RS10685 at 2263.975 kb on + strand, within DZA65_RS10685 at 2263.975 kb on + strand at 2263.975 kb on + strand, within DZA65_RS10685 at 2263.976 kb on - strand, within DZA65_RS10685 at 2263.976 kb on - strand, within DZA65_RS10685 at 2263.979 kb on + strand, within DZA65_RS10685 at 2263.980 kb on - strand, within DZA65_RS10685 at 2263.985 kb on + strand, within DZA65_RS10685 at 2263.985 kb on + strand, within DZA65_RS10685 at 2263.985 kb on + strand, within DZA65_RS10685 at 2263.986 kb on - strand, within DZA65_RS10685 at 2264.069 kb on + strand, within DZA65_RS10685 at 2264.069 kb on + strand, within DZA65_RS10685 at 2264.081 kb on - strand, within DZA65_RS10685 at 2264.113 kb on + strand, within DZA65_RS10685 at 2264.123 kb on + strand, within DZA65_RS10685 at 2264.123 kb on + strand, within DZA65_RS10685 at 2264.195 kb on + strand at 2264.208 kb on - strand at 2264.293 kb on - strand at 2264.356 kb on - strand at 2264.396 kb on - strand, within DZA65_RS10690 at 2264.396 kb on - strand, within DZA65_RS10690 at 2264.608 kb on - strand at 2264.608 kb on - strand at 2264.780 kb on - strand at 2264.783 kb on + strand at 2264.784 kb on - strand at 2264.802 kb on + strand at 2264.806 kb on + strand at 2264.807 kb on - strand at 2264.807 kb on - strand at 2264.826 kb on + strand at 2264.946 kb on - strand, within DZA65_RS22875 at 2264.968 kb on + strand at 2264.968 kb on + strand at 2264.977 kb on + strand at 2264.984 kb on - strand at 2265.037 kb on - strand at 2265.075 kb on - strand at 2265.100 kb on - strand at 2265.152 kb on - strand at 2265.154 kb on - strand at 2265.154 kb on - strand at 2265.154 kb on - strand at 2265.154 kb on - strand at 2265.171 kb on + strand at 2265.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,262,652 + +0.4 2,262,653 - +0.5 2,262,673 - +0.1 2,262,673 - -0.5 2,262,675 + +0.8 2,262,718 + DZA65_RS10675 0.10 -0.4 2,262,719 - DZA65_RS10675 0.11 -1.1 2,262,719 - DZA65_RS10675 0.11 -1.1 2,262,719 - DZA65_RS10675 0.11 +2.5 2,262,730 - DZA65_RS10675 0.13 -0.2 2,262,769 - DZA65_RS10675 0.23 -1.9 2,262,784 - +0.5 2,262,847 + DZA65_RS10675 0.43 +0.0 2,262,847 + DZA65_RS10675 0.43 -2.0 2,262,847 + DZA65_RS10675 0.43 +0.3 2,262,847 + DZA65_RS10675 0.43 -0.2 2,262,848 - DZA65_RS10675 0.43 -1.4 2,262,875 - DZA65_RS10675 0.50 +0.6 2,262,878 + DZA65_RS10675 0.50 +1.2 2,262,904 + DZA65_RS10675 0.57 +0.9 2,262,905 - DZA65_RS10675 0.57 -1.3 2,263,059 - -0.4 2,263,081 + -1.1 2,263,081 + +0.1 2,263,081 + +0.5 2,263,082 - -1.0 2,263,082 - +1.2 2,263,082 - -1.2 2,263,083 + +0.2 2,263,083 + +0.1 2,263,083 + +0.4 2,263,083 + -0.5 2,263,120 + -0.1 2,263,120 + +1.5 2,263,121 - +1.3 2,263,121 - +0.3 2,263,190 + -1.1 2,263,191 - -0.2 2,263,197 + -1.4 2,263,198 - -0.2 2,263,293 + DZA65_RS10680 0.50 +0.1 2,263,302 - DZA65_RS10680 0.57 -1.2 2,263,302 - DZA65_RS10680 0.57 +0.1 2,263,302 - DZA65_RS10680 0.57 -1.4 2,263,334 + DZA65_RS10680 0.83 -0.7 2,263,335 - DZA65_RS10680 0.83 +1.1 2,263,335 - DZA65_RS10680 0.83 +0.1 2,263,338 - DZA65_RS10680 0.86 +0.4 2,263,357 - +0.7 2,263,401 + -1.5 2,263,425 + +0.4 2,263,425 + +0.2 2,263,426 - -0.5 2,263,426 - +3.3 2,263,524 + -0.7 2,263,529 + +0.1 2,263,529 + +0.7 2,263,529 + -0.7 2,263,530 - -1.7 2,263,541 + +0.1 2,263,545 + -0.5 2,263,552 + -0.0 2,263,553 - -1.3 2,263,553 - +0.5 2,263,587 + +1.2 2,263,588 - -0.2 2,263,588 - +0.4 2,263,588 - +0.2 2,263,591 + +0.5 2,263,618 + -1.5 2,263,622 + +0.2 2,263,709 + DZA65_RS10685 0.14 -1.2 2,263,792 - DZA65_RS10685 0.28 -0.7 2,263,794 - DZA65_RS10685 0.28 -1.1 2,263,794 - DZA65_RS10685 0.28 +0.1 2,263,809 + DZA65_RS10685 0.31 +0.3 2,263,828 + DZA65_RS10685 0.34 +0.5 2,263,843 + DZA65_RS10685 0.36 -0.7 2,263,975 + DZA65_RS10685 0.58 -1.6 2,263,975 + +0.5 2,263,975 + DZA65_RS10685 0.58 -1.0 2,263,976 - DZA65_RS10685 0.58 +0.2 2,263,976 - DZA65_RS10685 0.58 -0.3 2,263,979 + DZA65_RS10685 0.59 -1.3 2,263,980 - DZA65_RS10685 0.59 -0.8 2,263,985 + DZA65_RS10685 0.60 -1.0 2,263,985 + DZA65_RS10685 0.60 -0.3 2,263,985 + DZA65_RS10685 0.60 -1.3 2,263,986 - DZA65_RS10685 0.60 +0.3 2,264,069 + DZA65_RS10685 0.74 -0.3 2,264,069 + DZA65_RS10685 0.74 -0.1 2,264,081 - DZA65_RS10685 0.76 +0.4 2,264,113 + DZA65_RS10685 0.81 +0.1 2,264,123 + DZA65_RS10685 0.83 +0.5 2,264,123 + DZA65_RS10685 0.83 +0.4 2,264,195 + -0.1 2,264,208 - -0.7 2,264,293 - -0.4 2,264,356 - -0.5 2,264,396 - DZA65_RS10690 0.12 -1.0 2,264,396 - DZA65_RS10690 0.12 +0.6 2,264,608 - -0.1 2,264,608 - -0.7 2,264,780 - -1.3 2,264,783 + -0.0 2,264,784 - -1.5 2,264,802 + +1.7 2,264,806 + +0.9 2,264,807 - -0.6 2,264,807 - -0.5 2,264,826 + +0.1 2,264,946 - DZA65_RS22875 0.76 -0.3 2,264,968 + +0.5 2,264,968 + +0.9 2,264,977 + -0.5 2,264,984 - +0.3 2,265,037 - -0.2 2,265,075 - +1.3 2,265,100 - -0.3 2,265,152 - -0.3 2,265,154 - +0.8 2,265,154 - -0.1 2,265,154 - -1.6 2,265,154 - -0.3 2,265,171 + +0.1 2,265,197 - +2.3
Or see this region's nucleotide sequence