Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10050 and DZA65_RS10055 overlap by 4 nucleotides DZA65_RS10055 and DZA65_RS10060 are separated by 4 nucleotides
DZA65_RS10050: DZA65_RS10050 - histidinol-phosphate transaminase, at 2,127,019 to 2,128,089
_RS10050
DZA65_RS10055: DZA65_RS10055 - histidinol dehydrogenase, at 2,128,086 to 2,129,396
_RS10055
DZA65_RS10060: DZA65_RS10060 - ATP phosphoribosyltransferase, at 2,129,401 to 2,130,300
_RS10060
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2127.221 kb on + strand, within DZA65_RS10050 at 2127.222 kb on - strand, within DZA65_RS10050 at 2127.223 kb on + strand, within DZA65_RS10050 at 2127.232 kb on + strand at 2127.232 kb on + strand, within DZA65_RS10050 at 2127.539 kb on + strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.648 kb on - strand, within DZA65_RS10050 at 2127.762 kb on - strand, within DZA65_RS10050 at 2127.770 kb on + strand, within DZA65_RS10050 at 2127.837 kb on + strand, within DZA65_RS10050 at 2127.838 kb on - strand, within DZA65_RS10050 at 2128.057 kb on + strand at 2128.058 kb on - strand at 2128.077 kb on + strand at 2128.078 kb on - strand at 2128.215 kb on + strand at 2128.258 kb on + strand, within DZA65_RS10055 at 2128.258 kb on + strand, within DZA65_RS10055 at 2128.342 kb on - strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2129.000 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.402 kb on + strand at 2129.436 kb on + strand at 2129.436 kb on + strand at 2129.437 kb on - strand at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.802 kb on + strand, within DZA65_RS10060 at 2129.803 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.928 kb on + strand, within DZA65_RS10060 at 2129.954 kb on + strand, within DZA65_RS10060 at 2129.955 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.280 kb on + strand at 2130.280 kb on + strand at 2130.281 kb on - strand at 2130.291 kb on + strand at 2130.292 kb on - strand at 2130.314 kb on + strand at 2130.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,127,221 + DZA65_RS10050 0.19 -2.6 2,127,222 - DZA65_RS10050 0.19 -2.6 2,127,223 + DZA65_RS10050 0.19 -2.8 2,127,232 + -1.5 2,127,232 + DZA65_RS10050 0.20 -0.1 2,127,539 + DZA65_RS10050 0.49 -1.8 2,127,540 - DZA65_RS10050 0.49 -2.1 2,127,540 - DZA65_RS10050 0.49 -0.9 2,127,647 + DZA65_RS10050 0.59 +1.0 2,127,647 + DZA65_RS10050 0.59 -1.5 2,127,647 + DZA65_RS10050 0.59 -1.8 2,127,648 - DZA65_RS10050 0.59 -0.5 2,127,762 - DZA65_RS10050 0.69 -2.5 2,127,770 + DZA65_RS10050 0.70 -1.7 2,127,837 + DZA65_RS10050 0.76 -2.8 2,127,838 - DZA65_RS10050 0.76 -2.3 2,128,057 + -2.6 2,128,058 - -2.1 2,128,077 + -0.7 2,128,078 - -2.1 2,128,215 + -1.3 2,128,258 + DZA65_RS10055 0.13 -1.6 2,128,258 + DZA65_RS10055 0.13 -2.5 2,128,342 - DZA65_RS10055 0.20 +0.4 2,128,753 + DZA65_RS10055 0.51 -1.3 2,128,753 + -2.5 2,128,753 + DZA65_RS10055 0.51 -1.8 2,128,753 + DZA65_RS10055 0.51 -3.8 2,128,753 + DZA65_RS10055 0.51 -2.9 2,128,754 - DZA65_RS10055 0.51 -0.2 2,128,754 - DZA65_RS10055 0.51 -0.6 2,129,000 + DZA65_RS10055 0.70 -0.2 2,129,002 + DZA65_RS10055 0.70 -3.4 2,129,002 + DZA65_RS10055 0.70 -3.3 2,129,002 + DZA65_RS10055 0.70 -1.3 2,129,003 - DZA65_RS10055 0.70 -2.1 2,129,003 - DZA65_RS10055 0.70 -4.0 2,129,402 + -2.5 2,129,436 + -1.1 2,129,436 + -1.3 2,129,437 - -1.6 2,129,618 + DZA65_RS10060 0.24 -0.9 2,129,618 + DZA65_RS10060 0.24 -3.1 2,129,619 - DZA65_RS10060 0.24 -0.1 2,129,619 - DZA65_RS10060 0.24 -0.1 2,129,619 - DZA65_RS10060 0.24 -0.1 2,129,619 - DZA65_RS10060 0.24 -0.3 2,129,619 - DZA65_RS10060 0.24 +0.3 2,129,736 - DZA65_RS10060 0.37 -0.9 2,129,736 - DZA65_RS10060 0.37 -0.6 2,129,802 + DZA65_RS10060 0.45 -2.5 2,129,803 - DZA65_RS10060 0.45 -0.3 2,129,889 - DZA65_RS10060 0.54 -1.1 2,129,889 - DZA65_RS10060 0.54 -3.4 2,129,928 + DZA65_RS10060 0.59 -1.1 2,129,954 + DZA65_RS10060 0.61 -2.3 2,129,955 - DZA65_RS10060 0.62 -2.1 2,130,024 - DZA65_RS10060 0.69 -1.8 2,130,024 - DZA65_RS10060 0.69 -2.8 2,130,280 + -0.3 2,130,280 + -0.2 2,130,281 - +0.8 2,130,291 + -0.7 2,130,292 - -1.1 2,130,314 + -0.1 2,130,335 + -0.3
Or see this region's nucleotide sequence