Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14860 and DZA65_RS14865 overlap by 14 nucleotides DZA65_RS14865 and DZA65_RS14870 are separated by 2 nucleotides DZA65_RS14870 and DZA65_RS14875 are separated by 64 nucleotides DZA65_RS14875 and DZA65_RS14880 overlap by 8 nucleotides DZA65_RS14880 and DZA65_RS14885 overlap by 11 nucleotides
DZA65_RS14860: DZA65_RS14860 - hydrogenase formation protein HypD, at 3,240,272 to 3,241,387
_RS14860
DZA65_RS14865: DZA65_RS14865 - hydrogenase maturation factor HybG, at 3,241,374 to 3,241,661
_RS14865
DZA65_RS14870: DZA65_RS14870 - hydrogenase nickel incorporation protein HypB, at 3,241,664 to 3,242,791
_RS14870
DZA65_RS14875: DZA65_RS14875 - hydrogenase maturation nickel metallochaperone HypA, at 3,242,856 to 3,243,197
_RS14875
DZA65_RS14880: DZA65_RS14880 - hydrogenase-2 assembly chaperone, at 3,243,190 to 3,243,756
_RS14880
DZA65_RS14885: DZA65_RS14885 - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,243,746 to 3,244,246
_RS14885
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3241.021 kb on - strand, within DZA65_RS14860 at 3241.090 kb on + strand, within DZA65_RS14860 at 3241.090 kb on + strand, within DZA65_RS14860 at 3241.091 kb on - strand, within DZA65_RS14860 at 3241.105 kb on + strand, within DZA65_RS14860 at 3241.309 kb on + strand at 3241.310 kb on - strand at 3241.310 kb on - strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.380 kb on + strand at 3241.381 kb on - strand at 3241.381 kb on - strand at 3241.381 kb on - strand at 3241.381 kb on - strand at 3241.381 kb on - strand at 3241.381 kb on - strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.387 kb on + strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.388 kb on - strand at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.509 kb on + strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.510 kb on - strand, within DZA65_RS14865 at 3241.565 kb on + strand, within DZA65_RS14865 at 3241.609 kb on + strand, within DZA65_RS14865 at 3241.665 kb on + strand at 3241.665 kb on + strand at 3241.666 kb on - strand at 3241.676 kb on + strand at 3241.677 kb on - strand at 3241.755 kb on - strand at 3241.755 kb on - strand at 3241.835 kb on + strand, within DZA65_RS14870 at 3241.835 kb on + strand, within DZA65_RS14870 at 3241.836 kb on - strand, within DZA65_RS14870 at 3241.836 kb on - strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.903 kb on + strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.904 kb on - strand, within DZA65_RS14870 at 3241.911 kb on + strand, within DZA65_RS14870 at 3241.911 kb on + strand, within DZA65_RS14870 at 3241.912 kb on - strand, within DZA65_RS14870 at 3241.912 kb on - strand, within DZA65_RS14870 at 3242.216 kb on - strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.226 kb on - strand, within DZA65_RS14870 at 3242.226 kb on - strand, within DZA65_RS14870 at 3242.345 kb on + strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.407 kb on - strand, within DZA65_RS14870 at 3242.571 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.605 kb on - strand, within DZA65_RS14870 at 3242.605 kb on - strand, within DZA65_RS14870 at 3242.642 kb on + strand, within DZA65_RS14870 at 3242.643 kb on - strand, within DZA65_RS14870 at 3242.643 kb on - strand, within DZA65_RS14870 at 3242.710 kb on + strand at 3242.711 kb on - strand at 3242.750 kb on + strand at 3242.751 kb on - strand at 3242.751 kb on - strand at 3242.751 kb on - strand at 3242.785 kb on + strand at 3242.795 kb on + strand at 3242.825 kb on + strand at 3242.825 kb on + strand at 3242.857 kb on + strand at 3242.912 kb on + strand, within DZA65_RS14875 at 3242.912 kb on + strand, within DZA65_RS14875 at 3242.912 kb on + strand, within DZA65_RS14875 at 3243.175 kb on + strand at 3243.175 kb on + strand at 3243.175 kb on + strand at 3243.368 kb on + strand, within DZA65_RS14880 at 3243.368 kb on + strand, within DZA65_RS14880 at 3243.369 kb on - strand, within DZA65_RS14880 at 3243.369 kb on - strand, within DZA65_RS14880 at 3243.484 kb on + strand, within DZA65_RS14880 at 3243.546 kb on + strand, within DZA65_RS14880 at 3243.547 kb on - strand, within DZA65_RS14880 at 3243.547 kb on - strand, within DZA65_RS14880 at 3243.600 kb on + strand, within DZA65_RS14880 at 3243.600 kb on + strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.697 kb on + strand, within DZA65_RS14880 at 3243.711 kb on + strand at 3243.711 kb on + strand at 3243.712 kb on - strand at 3243.712 kb on - strand at 3243.712 kb on - strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 3,241,021 - DZA65_RS14860 0.67 -2.0 3,241,090 + DZA65_RS14860 0.73 -0.2 3,241,090 + DZA65_RS14860 0.73 -0.1 3,241,091 - DZA65_RS14860 0.73 -2.3 3,241,105 + DZA65_RS14860 0.75 -0.5 3,241,309 + -1.0 3,241,310 - -0.4 3,241,310 - +0.4 3,241,380 + -1.6 3,241,380 + -1.1 3,241,380 + +0.1 3,241,380 + -0.5 3,241,380 + -0.8 3,241,380 + +1.1 3,241,380 + -0.1 3,241,381 - +1.5 3,241,381 - +0.1 3,241,381 - +2.0 3,241,381 - -0.7 3,241,381 - -2.0 3,241,381 - +0.6 3,241,387 + +0.2 3,241,387 + -0.5 3,241,387 + +0.1 3,241,387 + -0.4 3,241,387 + -0.6 3,241,387 + -2.0 3,241,387 + +0.5 3,241,388 - +0.5 3,241,388 - -0.9 3,241,388 - -0.7 3,241,388 - +0.8 3,241,388 - -0.0 3,241,388 - -1.5 3,241,388 - +0.8 3,241,388 - -0.4 3,241,388 - +0.0 3,241,388 - -0.1 3,241,388 - -1.5 3,241,509 + DZA65_RS14865 0.47 +0.3 3,241,509 + DZA65_RS14865 0.47 +0.3 3,241,509 + DZA65_RS14865 0.47 +0.2 3,241,509 + DZA65_RS14865 0.47 -1.0 3,241,509 + DZA65_RS14865 0.47 -0.5 3,241,509 + DZA65_RS14865 0.47 +1.0 3,241,509 + DZA65_RS14865 0.47 -0.4 3,241,509 + DZA65_RS14865 0.47 +0.2 3,241,509 + DZA65_RS14865 0.47 -2.3 3,241,509 + DZA65_RS14865 0.47 -2.2 3,241,509 + DZA65_RS14865 0.47 +0.8 3,241,509 + DZA65_RS14865 0.47 -0.1 3,241,509 + DZA65_RS14865 0.47 +0.1 3,241,509 + DZA65_RS14865 0.47 -0.9 3,241,509 + DZA65_RS14865 0.47 -0.6 3,241,510 - DZA65_RS14865 0.47 -0.7 3,241,510 - DZA65_RS14865 0.47 +0.1 3,241,510 - DZA65_RS14865 0.47 +0.8 3,241,510 - DZA65_RS14865 0.47 -0.3 3,241,510 - DZA65_RS14865 0.47 -0.2 3,241,510 - DZA65_RS14865 0.47 -0.1 3,241,510 - DZA65_RS14865 0.47 -0.6 3,241,565 + DZA65_RS14865 0.66 -1.2 3,241,609 + DZA65_RS14865 0.82 -0.3 3,241,665 + -0.5 3,241,665 + +0.8 3,241,666 - -0.8 3,241,676 + +0.3 3,241,677 - +0.6 3,241,755 - -0.9 3,241,755 - -0.9 3,241,835 + DZA65_RS14870 0.15 +0.5 3,241,835 + DZA65_RS14870 0.15 +0.7 3,241,836 - DZA65_RS14870 0.15 +0.5 3,241,836 - DZA65_RS14870 0.15 +1.6 3,241,903 + DZA65_RS14870 0.21 -0.2 3,241,903 + DZA65_RS14870 0.21 -0.4 3,241,903 + DZA65_RS14870 0.21 -0.3 3,241,903 + DZA65_RS14870 0.21 -1.3 3,241,903 + DZA65_RS14870 0.21 -0.5 3,241,903 + DZA65_RS14870 0.21 +0.5 3,241,903 + DZA65_RS14870 0.21 -0.6 3,241,903 + DZA65_RS14870 0.21 +0.1 3,241,903 + DZA65_RS14870 0.21 -1.3 3,241,903 + DZA65_RS14870 0.21 +0.6 3,241,903 + DZA65_RS14870 0.21 -0.8 3,241,903 + DZA65_RS14870 0.21 -0.9 3,241,903 + DZA65_RS14870 0.21 -1.6 3,241,903 + DZA65_RS14870 0.21 -0.1 3,241,903 + DZA65_RS14870 0.21 -0.6 3,241,903 + DZA65_RS14870 0.21 -0.3 3,241,903 + DZA65_RS14870 0.21 -0.0 3,241,903 + DZA65_RS14870 0.21 -1.4 3,241,903 + DZA65_RS14870 0.21 -1.5 3,241,903 + DZA65_RS14870 0.21 -0.4 3,241,903 + DZA65_RS14870 0.21 -1.0 3,241,904 - DZA65_RS14870 0.21 -0.3 3,241,904 - DZA65_RS14870 0.21 -0.7 3,241,904 - DZA65_RS14870 0.21 -2.9 3,241,904 - -0.2 3,241,904 - DZA65_RS14870 0.21 +0.4 3,241,904 - DZA65_RS14870 0.21 -1.3 3,241,904 - DZA65_RS14870 0.21 -1.0 3,241,904 - -1.2 3,241,904 - DZA65_RS14870 0.21 -0.9 3,241,904 - DZA65_RS14870 0.21 -0.6 3,241,904 - DZA65_RS14870 0.21 +0.1 3,241,904 - DZA65_RS14870 0.21 +1.1 3,241,904 - DZA65_RS14870 0.21 -0.2 3,241,904 - DZA65_RS14870 0.21 -0.9 3,241,904 - DZA65_RS14870 0.21 -0.2 3,241,904 - DZA65_RS14870 0.21 -0.5 3,241,904 - DZA65_RS14870 0.21 -0.6 3,241,904 - DZA65_RS14870 0.21 -0.3 3,241,904 - DZA65_RS14870 0.21 +0.1 3,241,904 - DZA65_RS14870 0.21 -0.9 3,241,911 + DZA65_RS14870 0.22 -2.0 3,241,911 + DZA65_RS14870 0.22 -0.8 3,241,912 - DZA65_RS14870 0.22 +0.8 3,241,912 - DZA65_RS14870 0.22 +0.0 3,242,216 - DZA65_RS14870 0.49 +0.1 3,242,225 + DZA65_RS14870 0.50 -0.4 3,242,225 + DZA65_RS14870 0.50 +0.1 3,242,225 + DZA65_RS14870 0.50 -0.1 3,242,226 - DZA65_RS14870 0.50 -1.2 3,242,226 - DZA65_RS14870 0.50 -0.0 3,242,345 + DZA65_RS14870 0.60 -0.1 3,242,346 - DZA65_RS14870 0.60 -0.7 3,242,346 - DZA65_RS14870 0.60 +0.1 3,242,346 - DZA65_RS14870 0.60 +0.5 3,242,407 - DZA65_RS14870 0.66 -0.9 3,242,571 + DZA65_RS14870 0.80 -1.0 3,242,598 + DZA65_RS14870 0.83 +0.3 3,242,598 + DZA65_RS14870 0.83 -1.2 3,242,598 + DZA65_RS14870 0.83 -0.2 3,242,599 - DZA65_RS14870 0.83 -0.7 3,242,599 - DZA65_RS14870 0.83 -0.2 3,242,599 - DZA65_RS14870 0.83 +0.6 3,242,599 - -0.8 3,242,599 - DZA65_RS14870 0.83 -0.0 3,242,601 + DZA65_RS14870 0.83 -0.6 3,242,601 + DZA65_RS14870 0.83 -0.7 3,242,601 + DZA65_RS14870 0.83 -0.2 3,242,601 + DZA65_RS14870 0.83 -0.1 3,242,601 + DZA65_RS14870 0.83 -0.2 3,242,601 + DZA65_RS14870 0.83 -1.2 3,242,601 + DZA65_RS14870 0.83 +0.8 3,242,601 + DZA65_RS14870 0.83 +0.4 3,242,602 - DZA65_RS14870 0.83 +0.2 3,242,602 - DZA65_RS14870 0.83 -0.9 3,242,602 - DZA65_RS14870 0.83 +0.4 3,242,602 - DZA65_RS14870 0.83 +0.8 3,242,602 - DZA65_RS14870 0.83 -0.9 3,242,602 - DZA65_RS14870 0.83 -1.2 3,242,602 - DZA65_RS14870 0.83 -1.5 3,242,602 - DZA65_RS14870 0.83 -2.0 3,242,602 - DZA65_RS14870 0.83 +0.4 3,242,602 - DZA65_RS14870 0.83 -0.6 3,242,602 - -0.2 3,242,602 - DZA65_RS14870 0.83 -1.9 3,242,602 - DZA65_RS14870 0.83 +0.3 3,242,602 - DZA65_RS14870 0.83 -2.2 3,242,604 + DZA65_RS14870 0.83 -1.8 3,242,604 + DZA65_RS14870 0.83 -0.4 3,242,604 + DZA65_RS14870 0.83 +0.1 3,242,604 + DZA65_RS14870 0.83 -0.2 3,242,605 - DZA65_RS14870 0.83 -0.6 3,242,605 - DZA65_RS14870 0.83 -0.6 3,242,642 + DZA65_RS14870 0.87 -0.9 3,242,643 - DZA65_RS14870 0.87 -0.6 3,242,643 - DZA65_RS14870 0.87 -0.4 3,242,710 + +1.6 3,242,711 - +1.6 3,242,750 + +0.6 3,242,751 - +0.2 3,242,751 - -0.8 3,242,751 - -0.4 3,242,785 + +0.2 3,242,795 + +0.0 3,242,825 + +0.1 3,242,825 + -0.2 3,242,857 + +0.1 3,242,912 + DZA65_RS14875 0.16 +0.1 3,242,912 + DZA65_RS14875 0.16 +0.5 3,242,912 + DZA65_RS14875 0.16 +0.2 3,243,175 + -0.6 3,243,175 + -0.1 3,243,175 + -0.8 3,243,368 + DZA65_RS14880 0.31 -0.2 3,243,368 + DZA65_RS14880 0.31 -3.1 3,243,369 - DZA65_RS14880 0.32 -0.7 3,243,369 - DZA65_RS14880 0.32 +0.1 3,243,484 + DZA65_RS14880 0.52 -0.1 3,243,546 + DZA65_RS14880 0.63 -0.3 3,243,547 - DZA65_RS14880 0.63 -2.3 3,243,547 - DZA65_RS14880 0.63 -0.2 3,243,600 + DZA65_RS14880 0.72 +0.2 3,243,600 + DZA65_RS14880 0.72 +0.2 3,243,601 - DZA65_RS14880 0.72 +0.8 3,243,601 - DZA65_RS14880 0.72 +0.8 3,243,601 - DZA65_RS14880 0.72 -0.8 3,243,601 - DZA65_RS14880 0.72 +1.0 3,243,697 + DZA65_RS14880 0.89 -0.1 3,243,711 + -0.3 3,243,711 + -0.0 3,243,712 - +0.0 3,243,712 - -0.1 3,243,712 - -0.6 3,243,756 + +0.5 3,243,756 + -1.8 3,243,756 + -0.2 3,243,756 + +0.1 3,243,756 + +0.0 3,243,756 + -0.3 3,243,756 + -0.3 3,243,756 + +0.5 3,243,756 + -0.4 3,243,756 + -0.1 3,243,756 + +0.4 3,243,757 - -0.8 3,243,757 - -0.2 3,243,757 - +0.6 3,243,757 - -0.3 3,243,757 - -0.9 3,243,757 - -0.7 3,243,757 - +0.1 3,243,757 - +2.1 3,243,757 - -0.3 3,243,757 - +0.6 3,243,757 - +0.4 3,243,757 - -0.4
Or see this region's nucleotide sequence