Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14870 and DZA65_RS14875 are separated by 64 nucleotides DZA65_RS14875 and DZA65_RS14880 overlap by 8 nucleotides DZA65_RS14880 and DZA65_RS14885 overlap by 11 nucleotides DZA65_RS14885 and DZA65_RS14890 overlap by 1 nucleotides
DZA65_RS14870: DZA65_RS14870 - hydrogenase nickel incorporation protein HypB, at 3,241,664 to 3,242,791
_RS14870
DZA65_RS14875: DZA65_RS14875 - hydrogenase maturation nickel metallochaperone HypA, at 3,242,856 to 3,243,197
_RS14875
DZA65_RS14880: DZA65_RS14880 - hydrogenase-2 assembly chaperone, at 3,243,190 to 3,243,756
_RS14880
DZA65_RS14885: DZA65_RS14885 - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,243,746 to 3,244,246
_RS14885
DZA65_RS14890: DZA65_RS14890 - hydrogenase 2 large subunit, at 3,244,246 to 3,245,952
_RS14890
Position (kb)
3243
3244 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3242.216 kb on - strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.225 kb on + strand, within DZA65_RS14870 at 3242.226 kb on - strand, within DZA65_RS14870 at 3242.226 kb on - strand, within DZA65_RS14870 at 3242.345 kb on + strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.346 kb on - strand, within DZA65_RS14870 at 3242.407 kb on - strand, within DZA65_RS14870 at 3242.571 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.598 kb on + strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.599 kb on - strand at 3242.599 kb on - strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.601 kb on + strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.602 kb on - strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.604 kb on + strand, within DZA65_RS14870 at 3242.605 kb on - strand, within DZA65_RS14870 at 3242.605 kb on - strand, within DZA65_RS14870 at 3242.642 kb on + strand, within DZA65_RS14870 at 3242.643 kb on - strand, within DZA65_RS14870 at 3242.643 kb on - strand, within DZA65_RS14870 at 3242.710 kb on + strand at 3242.711 kb on - strand at 3242.750 kb on + strand at 3242.751 kb on - strand at 3242.751 kb on - strand at 3242.751 kb on - strand at 3242.785 kb on + strand at 3242.795 kb on + strand at 3242.825 kb on + strand at 3242.825 kb on + strand at 3242.857 kb on + strand at 3242.912 kb on + strand, within DZA65_RS14875 at 3242.912 kb on + strand, within DZA65_RS14875 at 3242.912 kb on + strand, within DZA65_RS14875 at 3243.175 kb on + strand at 3243.175 kb on + strand at 3243.175 kb on + strand at 3243.368 kb on + strand, within DZA65_RS14880 at 3243.368 kb on + strand, within DZA65_RS14880 at 3243.369 kb on - strand, within DZA65_RS14880 at 3243.369 kb on - strand, within DZA65_RS14880 at 3243.484 kb on + strand, within DZA65_RS14880 at 3243.546 kb on + strand, within DZA65_RS14880 at 3243.547 kb on - strand, within DZA65_RS14880 at 3243.547 kb on - strand, within DZA65_RS14880 at 3243.600 kb on + strand, within DZA65_RS14880 at 3243.600 kb on + strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.601 kb on - strand, within DZA65_RS14880 at 3243.697 kb on + strand, within DZA65_RS14880 at 3243.711 kb on + strand at 3243.711 kb on + strand at 3243.712 kb on - strand at 3243.712 kb on - strand at 3243.712 kb on - strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.756 kb on + strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.817 kb on + strand, within DZA65_RS14885 at 3243.818 kb on - strand, within DZA65_RS14885 at 3243.818 kb on - strand, within DZA65_RS14885 at 3243.818 kb on - strand, within DZA65_RS14885 at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.238 kb on + strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.239 kb on - strand at 3244.359 kb on + strand at 3244.360 kb on - strand at 3244.360 kb on - strand at 3244.413 kb on + strand at 3244.466 kb on + strand, within DZA65_RS14890 at 3244.466 kb on + strand, within DZA65_RS14890 at 3244.466 kb on + strand, within DZA65_RS14890 at 3244.466 kb on + strand, within DZA65_RS14890 at 3244.467 kb on - strand, within DZA65_RS14890 at 3244.467 kb on - strand, within DZA65_RS14890 at 3244.475 kb on - strand, within DZA65_RS14890 at 3244.475 kb on - strand, within DZA65_RS14890 at 3244.488 kb on + strand, within DZA65_RS14890 at 3244.651 kb on + strand, within DZA65_RS14890 at 3244.652 kb on - strand, within DZA65_RS14890 at 3244.669 kb on - strand, within DZA65_RS14890 at 3244.750 kb on + strand, within DZA65_RS14890
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,242,216 - DZA65_RS14870 0.49 +0.0 3,242,225 + DZA65_RS14870 0.50 +1.2 3,242,225 + DZA65_RS14870 0.50 +0.3 3,242,225 + DZA65_RS14870 0.50 -0.1 3,242,226 - DZA65_RS14870 0.50 -0.1 3,242,226 - DZA65_RS14870 0.50 -1.0 3,242,345 + DZA65_RS14870 0.60 -0.3 3,242,346 - DZA65_RS14870 0.60 -0.1 3,242,346 - DZA65_RS14870 0.60 -0.4 3,242,346 - DZA65_RS14870 0.60 -1.1 3,242,407 - DZA65_RS14870 0.66 -1.5 3,242,571 + DZA65_RS14870 0.80 +1.0 3,242,598 + DZA65_RS14870 0.83 +0.7 3,242,598 + DZA65_RS14870 0.83 -2.1 3,242,598 + DZA65_RS14870 0.83 -1.8 3,242,599 - DZA65_RS14870 0.83 -0.9 3,242,599 - DZA65_RS14870 0.83 +0.2 3,242,599 - DZA65_RS14870 0.83 +0.8 3,242,599 - -0.4 3,242,599 - DZA65_RS14870 0.83 +0.0 3,242,601 + DZA65_RS14870 0.83 -1.2 3,242,601 + DZA65_RS14870 0.83 -1.3 3,242,601 + DZA65_RS14870 0.83 +0.5 3,242,601 + DZA65_RS14870 0.83 +0.2 3,242,601 + DZA65_RS14870 0.83 -1.1 3,242,601 + DZA65_RS14870 0.83 -1.4 3,242,601 + DZA65_RS14870 0.83 +0.9 3,242,601 + DZA65_RS14870 0.83 +0.4 3,242,602 - DZA65_RS14870 0.83 +0.6 3,242,602 - DZA65_RS14870 0.83 -0.1 3,242,602 - DZA65_RS14870 0.83 +0.2 3,242,602 - DZA65_RS14870 0.83 +0.5 3,242,602 - DZA65_RS14870 0.83 -0.2 3,242,602 - DZA65_RS14870 0.83 -1.8 3,242,602 - DZA65_RS14870 0.83 -2.1 3,242,602 - DZA65_RS14870 0.83 -1.6 3,242,602 - DZA65_RS14870 0.83 -0.7 3,242,602 - DZA65_RS14870 0.83 +0.3 3,242,602 - -0.8 3,242,602 - DZA65_RS14870 0.83 -0.9 3,242,602 - DZA65_RS14870 0.83 -2.1 3,242,602 - DZA65_RS14870 0.83 -0.8 3,242,604 + DZA65_RS14870 0.83 +0.2 3,242,604 + DZA65_RS14870 0.83 -0.6 3,242,604 + DZA65_RS14870 0.83 -0.2 3,242,604 + DZA65_RS14870 0.83 +0.2 3,242,605 - DZA65_RS14870 0.83 -0.8 3,242,605 - DZA65_RS14870 0.83 +0.5 3,242,642 + DZA65_RS14870 0.87 -0.1 3,242,643 - DZA65_RS14870 0.87 -0.3 3,242,643 - DZA65_RS14870 0.87 -0.2 3,242,710 + +1.2 3,242,711 - +0.2 3,242,750 + -0.2 3,242,751 - +0.1 3,242,751 - -0.7 3,242,751 - +0.4 3,242,785 + -0.4 3,242,795 + +0.1 3,242,825 + -0.1 3,242,825 + -0.8 3,242,857 + -0.5 3,242,912 + DZA65_RS14875 0.16 +0.2 3,242,912 + DZA65_RS14875 0.16 -0.1 3,242,912 + DZA65_RS14875 0.16 +0.3 3,243,175 + +0.5 3,243,175 + -0.5 3,243,175 + -1.0 3,243,368 + DZA65_RS14880 0.31 -1.8 3,243,368 + DZA65_RS14880 0.31 -0.7 3,243,369 - DZA65_RS14880 0.32 -0.4 3,243,369 - DZA65_RS14880 0.32 -1.8 3,243,484 + DZA65_RS14880 0.52 -0.9 3,243,546 + DZA65_RS14880 0.63 -0.3 3,243,547 - DZA65_RS14880 0.63 -1.0 3,243,547 - DZA65_RS14880 0.63 -2.1 3,243,600 + DZA65_RS14880 0.72 -0.1 3,243,600 + DZA65_RS14880 0.72 +0.6 3,243,601 - DZA65_RS14880 0.72 -0.8 3,243,601 - DZA65_RS14880 0.72 -0.1 3,243,601 - DZA65_RS14880 0.72 -2.1 3,243,601 - DZA65_RS14880 0.72 +0.1 3,243,697 + DZA65_RS14880 0.89 -0.5 3,243,711 + +0.1 3,243,711 + -1.0 3,243,712 - -0.6 3,243,712 - -0.5 3,243,712 - -1.3 3,243,756 + -0.2 3,243,756 + -1.4 3,243,756 + -0.4 3,243,756 + -0.4 3,243,756 + +0.2 3,243,756 + +0.1 3,243,756 + -0.1 3,243,756 + +1.3 3,243,756 + -0.5 3,243,756 + -0.9 3,243,756 + -0.8 3,243,757 - -0.4 3,243,757 - +1.2 3,243,757 - -0.8 3,243,757 - -0.4 3,243,757 - -1.1 3,243,757 - -0.1 3,243,757 - +0.5 3,243,757 - +1.8 3,243,757 - +0.3 3,243,757 - -0.6 3,243,757 - +0.3 3,243,757 - -0.1 3,243,817 + DZA65_RS14885 0.14 -0.7 3,243,818 - DZA65_RS14885 0.14 -0.2 3,243,818 - DZA65_RS14885 0.14 -0.0 3,243,818 - DZA65_RS14885 0.14 +0.2 3,244,238 + -2.1 3,244,238 + -0.5 3,244,238 + -0.2 3,244,238 + -1.1 3,244,238 + +0.7 3,244,238 + -2.8 3,244,238 + -1.4 3,244,238 + -0.4 3,244,238 + +0.5 3,244,239 - +0.2 3,244,239 - -0.9 3,244,239 - -0.5 3,244,239 - +0.6 3,244,239 - -0.5 3,244,239 - +0.3 3,244,239 - +0.0 3,244,359 + +0.9 3,244,360 - -0.4 3,244,360 - -0.8 3,244,413 + +0.8 3,244,466 + DZA65_RS14890 0.13 -0.3 3,244,466 + DZA65_RS14890 0.13 -1.8 3,244,466 + DZA65_RS14890 0.13 -0.9 3,244,466 + DZA65_RS14890 0.13 +0.1 3,244,467 - DZA65_RS14890 0.13 -0.7 3,244,467 - DZA65_RS14890 0.13 +0.3 3,244,475 - DZA65_RS14890 0.13 -0.0 3,244,475 - DZA65_RS14890 0.13 +0.5 3,244,488 + DZA65_RS14890 0.14 -0.8 3,244,651 + DZA65_RS14890 0.24 -0.5 3,244,652 - DZA65_RS14890 0.24 -0.2 3,244,669 - DZA65_RS14890 0.25 -0.0 3,244,750 + DZA65_RS14890 0.30 -1.7
Or see this region's nucleotide sequence