Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS21120 and DZA65_RS21125 overlap by 4 nucleotides DZA65_RS21125 and DZA65_RS21130 are separated by 74 nucleotides DZA65_RS21130 and DZA65_RS21135 are separated by 2 nucleotides
DZA65_RS21120: DZA65_RS21120 - hydroxymethylbilane synthase, at 4,566,006 to 4,566,947
_RS21120
DZA65_RS21125: DZA65_RS21125 - uroporphyrinogen-III synthase, at 4,566,944 to 4,567,684
_RS21125
DZA65_RS21130: DZA65_RS21130 - uroporphyrinogen-III C-methyltransferase, at 4,567,759 to 4,568,886
_RS21130
DZA65_RS21135: DZA65_RS21135 - protoheme IX biogenesis protein HemY, at 4,568,889 to 4,570,085
_RS21135
Position (kb)
4567
4568
4569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4567.708 kb on + strand at 4567.709 kb on - strand at 4567.728 kb on + strand at 4567.728 kb on + strand at 4567.728 kb on + strand at 4567.729 kb on - strand at 4567.729 kb on - strand at 4567.729 kb on - strand at 4567.729 kb on - strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.745 kb on + strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.746 kb on - strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.752 kb on + strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.753 kb on - strand at 4567.758 kb on + strand at 4567.758 kb on + strand at 4567.759 kb on - strand at 4567.759 kb on - strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.773 kb on + strand at 4567.774 kb on - strand at 4567.774 kb on - strand at 4567.774 kb on - strand at 4567.774 kb on - strand at 4567.774 kb on - strand at 4567.921 kb on + strand, within DZA65_RS21130 at 4567.921 kb on + strand, within DZA65_RS21130 at 4567.921 kb on + strand, within DZA65_RS21130 at 4567.922 kb on - strand, within DZA65_RS21130 at 4567.922 kb on - strand, within DZA65_RS21130 at 4567.924 kb on + strand, within DZA65_RS21130 at 4567.924 kb on + strand, within DZA65_RS21130 at 4567.924 kb on + strand, within DZA65_RS21130 at 4567.924 kb on + strand, within DZA65_RS21130 at 4567.925 kb on - strand, within DZA65_RS21130 at 4567.925 kb on - strand, within DZA65_RS21130 at 4567.925 kb on - strand, within DZA65_RS21130 at 4567.927 kb on + strand, within DZA65_RS21130 at 4567.927 kb on + strand, within DZA65_RS21130 at 4567.927 kb on + strand, within DZA65_RS21130 at 4567.928 kb on - strand, within DZA65_RS21130 at 4568.149 kb on - strand, within DZA65_RS21130 at 4568.149 kb on - strand, within DZA65_RS21130 at 4568.370 kb on + strand, within DZA65_RS21130 at 4568.370 kb on + strand, within DZA65_RS21130 at 4568.396 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.590 kb on + strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.591 kb on - strand, within DZA65_RS21130 at 4568.697 kb on - strand, within DZA65_RS21130 at 4568.786 kb on + strand at 4568.786 kb on + strand at 4568.787 kb on - strand at 4568.787 kb on - strand at 4568.899 kb on + strand at 4568.899 kb on + strand at 4568.899 kb on + strand at 4568.899 kb on + strand at 4568.899 kb on + strand at 4568.900 kb on - strand at 4568.900 kb on - strand at 4568.900 kb on - strand at 4568.967 kb on + strand at 4568.967 kb on + strand at 4568.967 kb on + strand at 4568.968 kb on - strand at 4568.968 kb on - strand at 4568.991 kb on + strand at 4568.991 kb on + strand at 4568.991 kb on + strand at 4568.991 kb on + strand at 4568.991 kb on + strand at 4568.992 kb on - strand at 4569.126 kb on - strand, within DZA65_RS21135 at 4569.186 kb on + strand, within DZA65_RS21135 at 4569.187 kb on - strand, within DZA65_RS21135 at 4569.187 kb on - strand, within DZA65_RS21135 at 4569.187 kb on - strand, within DZA65_RS21135 at 4569.249 kb on + strand, within DZA65_RS21135 at 4569.249 kb on + strand, within DZA65_RS21135 at 4569.249 kb on + strand, within DZA65_RS21135 at 4569.249 kb on + strand, within DZA65_RS21135 at 4569.250 kb on - strand, within DZA65_RS21135 at 4569.250 kb on - strand, within DZA65_RS21135 at 4569.250 kb on - strand, within DZA65_RS21135 at 4569.250 kb on - strand, within DZA65_RS21135 at 4569.306 kb on + strand, within DZA65_RS21135 at 4569.306 kb on + strand, within DZA65_RS21135 at 4569.416 kb on - strand at 4569.495 kb on + strand, within DZA65_RS21135 at 4569.495 kb on + strand, within DZA65_RS21135 at 4569.496 kb on - strand, within DZA65_RS21135 at 4569.512 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.530 kb on + strand, within DZA65_RS21135 at 4569.531 kb on - strand, within DZA65_RS21135 at 4569.531 kb on - strand, within DZA65_RS21135 at 4569.555 kb on + strand, within DZA65_RS21135 at 4569.555 kb on + strand, within DZA65_RS21135 at 4569.555 kb on + strand, within DZA65_RS21135 at 4569.585 kb on - strand, within DZA65_RS21135 at 4569.659 kb on + strand, within DZA65_RS21135 at 4569.659 kb on + strand, within DZA65_RS21135 at 4569.660 kb on - strand, within DZA65_RS21135 at 4569.660 kb on - strand, within DZA65_RS21135 at 4569.660 kb on - strand, within DZA65_RS21135 at 4569.660 kb on - strand, within DZA65_RS21135 at 4569.748 kb on + strand, within DZA65_RS21135 at 4569.748 kb on + strand, within DZA65_RS21135 at 4569.748 kb on + strand, within DZA65_RS21135 at 4569.748 kb on + strand, within DZA65_RS21135 at 4569.749 kb on - strand, within DZA65_RS21135 at 4569.784 kb on + strand at 4569.785 kb on - strand, within DZA65_RS21135 at 4569.884 kb on + strand, within DZA65_RS21135 at 4569.885 kb on - strand, within DZA65_RS21135 at 4569.885 kb on - strand, within DZA65_RS21135 at 4569.885 kb on - strand, within DZA65_RS21135 at 4569.885 kb on - strand, within DZA65_RS21135 at 4569.885 kb on - strand, within DZA65_RS21135
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,567,708 + -0.1 4,567,709 - +0.3 4,567,728 + +0.6 4,567,728 + +0.3 4,567,728 + -0.1 4,567,729 - -0.4 4,567,729 - -0.5 4,567,729 - +0.2 4,567,729 - -1.6 4,567,745 + -0.0 4,567,745 + -0.4 4,567,745 + +0.2 4,567,745 + +0.3 4,567,745 + +0.6 4,567,745 + -1.0 4,567,745 + +0.6 4,567,745 + -0.4 4,567,746 - +0.1 4,567,746 - -1.2 4,567,746 - -0.3 4,567,746 - -1.9 4,567,746 - -0.9 4,567,746 - +0.8 4,567,746 - -1.5 4,567,746 - +0.2 4,567,746 - +0.2 4,567,746 - -1.0 4,567,746 - -0.0 4,567,746 - -0.9 4,567,752 + -0.4 4,567,752 + -0.2 4,567,752 + +2.6 4,567,752 + +0.1 4,567,752 + -0.7 4,567,752 + -0.7 4,567,752 + -1.1 4,567,752 + -1.1 4,567,752 + -0.8 4,567,752 + -0.2 4,567,752 + +0.8 4,567,752 + -0.3 4,567,752 + +0.1 4,567,752 + -0.7 4,567,752 + -0.3 4,567,752 + +0.3 4,567,752 + -0.6 4,567,752 + -0.5 4,567,752 + -0.2 4,567,752 + -1.3 4,567,752 + +0.4 4,567,752 + -0.7 4,567,752 + -1.0 4,567,752 + +1.0 4,567,752 + -1.4 4,567,752 + +0.1 4,567,752 + -1.9 4,567,752 + -0.2 4,567,753 - -1.9 4,567,753 - -0.6 4,567,753 - +0.6 4,567,753 - -1.4 4,567,753 - +0.3 4,567,753 - -1.6 4,567,753 - +0.2 4,567,753 - +0.1 4,567,753 - +0.1 4,567,753 - +1.2 4,567,753 - -0.9 4,567,753 - -1.0 4,567,758 + +0.1 4,567,758 + +2.7 4,567,759 - +0.2 4,567,759 - +0.1 4,567,773 + -1.4 4,567,773 + -1.0 4,567,773 + -0.1 4,567,773 + +0.2 4,567,773 + +0.9 4,567,773 + +0.8 4,567,773 + -0.9 4,567,773 + -0.2 4,567,773 + -0.2 4,567,774 - -0.0 4,567,774 - +0.7 4,567,774 - -0.2 4,567,774 - -0.6 4,567,774 - -0.1 4,567,921 + DZA65_RS21130 0.14 -2.3 4,567,921 + DZA65_RS21130 0.14 +0.3 4,567,921 + DZA65_RS21130 0.14 +0.8 4,567,922 - DZA65_RS21130 0.14 -0.2 4,567,922 - DZA65_RS21130 0.14 -1.8 4,567,924 + DZA65_RS21130 0.15 -0.5 4,567,924 + DZA65_RS21130 0.15 -0.4 4,567,924 + DZA65_RS21130 0.15 +0.8 4,567,924 + DZA65_RS21130 0.15 -0.2 4,567,925 - DZA65_RS21130 0.15 -1.0 4,567,925 - DZA65_RS21130 0.15 +0.1 4,567,925 - DZA65_RS21130 0.15 +0.9 4,567,927 + DZA65_RS21130 0.15 -1.1 4,567,927 + DZA65_RS21130 0.15 -1.2 4,567,927 + DZA65_RS21130 0.15 -1.6 4,567,928 - DZA65_RS21130 0.15 -0.4 4,568,149 - DZA65_RS21130 0.35 -0.9 4,568,149 - DZA65_RS21130 0.35 +0.6 4,568,370 + DZA65_RS21130 0.54 -0.4 4,568,370 + DZA65_RS21130 0.54 +0.1 4,568,396 + DZA65_RS21130 0.56 -0.6 4,568,590 + DZA65_RS21130 0.74 -0.9 4,568,590 + DZA65_RS21130 0.74 -0.0 4,568,590 + DZA65_RS21130 0.74 -1.4 4,568,590 + DZA65_RS21130 0.74 -1.6 4,568,590 + DZA65_RS21130 0.74 -1.4 4,568,590 + DZA65_RS21130 0.74 -0.1 4,568,590 + DZA65_RS21130 0.74 -0.9 4,568,590 + DZA65_RS21130 0.74 -0.2 4,568,590 + DZA65_RS21130 0.74 -0.9 4,568,591 - DZA65_RS21130 0.74 -1.2 4,568,591 - DZA65_RS21130 0.74 -0.4 4,568,591 - DZA65_RS21130 0.74 -1.2 4,568,591 - DZA65_RS21130 0.74 +0.5 4,568,591 - DZA65_RS21130 0.74 -0.4 4,568,591 - DZA65_RS21130 0.74 +0.3 4,568,591 - DZA65_RS21130 0.74 -0.7 4,568,591 - DZA65_RS21130 0.74 -1.2 4,568,697 - DZA65_RS21130 0.83 +0.0 4,568,786 + -1.0 4,568,786 + +0.0 4,568,787 - -0.9 4,568,787 - -0.7 4,568,899 + +1.6 4,568,899 + +1.0 4,568,899 + -0.4 4,568,899 + -0.7 4,568,899 + +0.2 4,568,900 - -3.0 4,568,900 - +0.2 4,568,900 - +0.9 4,568,967 + -1.2 4,568,967 + -0.5 4,568,967 + -0.2 4,568,968 - -2.0 4,568,968 - +0.3 4,568,991 + +0.1 4,568,991 + -1.2 4,568,991 + -1.9 4,568,991 + -0.4 4,568,991 + -0.8 4,568,992 - +0.8 4,569,126 - DZA65_RS21135 0.20 -0.2 4,569,186 + DZA65_RS21135 0.25 +0.6 4,569,187 - DZA65_RS21135 0.25 -0.1 4,569,187 - DZA65_RS21135 0.25 -0.7 4,569,187 - DZA65_RS21135 0.25 +1.6 4,569,249 + DZA65_RS21135 0.30 -1.1 4,569,249 + DZA65_RS21135 0.30 +0.1 4,569,249 + DZA65_RS21135 0.30 -0.6 4,569,249 + DZA65_RS21135 0.30 -1.2 4,569,250 - DZA65_RS21135 0.30 -0.2 4,569,250 - DZA65_RS21135 0.30 -0.2 4,569,250 - DZA65_RS21135 0.30 -1.8 4,569,250 - DZA65_RS21135 0.30 -0.8 4,569,306 + DZA65_RS21135 0.35 -0.2 4,569,306 + DZA65_RS21135 0.35 +0.1 4,569,416 - +1.0 4,569,495 + DZA65_RS21135 0.51 +0.3 4,569,495 + DZA65_RS21135 0.51 +1.0 4,569,496 - DZA65_RS21135 0.51 +0.7 4,569,512 + DZA65_RS21135 0.52 -0.7 4,569,530 + DZA65_RS21135 0.54 +1.6 4,569,530 + DZA65_RS21135 0.54 +0.2 4,569,530 + DZA65_RS21135 0.54 -0.3 4,569,530 + DZA65_RS21135 0.54 -0.0 4,569,530 + DZA65_RS21135 0.54 -2.3 4,569,530 + DZA65_RS21135 0.54 -0.8 4,569,531 - DZA65_RS21135 0.54 -0.0 4,569,531 - DZA65_RS21135 0.54 -0.8 4,569,555 + DZA65_RS21135 0.56 +1.9 4,569,555 + DZA65_RS21135 0.56 -0.8 4,569,555 + DZA65_RS21135 0.56 -0.4 4,569,585 - DZA65_RS21135 0.58 -0.3 4,569,659 + DZA65_RS21135 0.64 +1.0 4,569,659 + DZA65_RS21135 0.64 -1.0 4,569,660 - DZA65_RS21135 0.64 -0.4 4,569,660 - DZA65_RS21135 0.64 -0.0 4,569,660 - DZA65_RS21135 0.64 -0.6 4,569,660 - DZA65_RS21135 0.64 -0.8 4,569,748 + DZA65_RS21135 0.72 -0.1 4,569,748 + DZA65_RS21135 0.72 -1.4 4,569,748 + DZA65_RS21135 0.72 +0.1 4,569,748 + DZA65_RS21135 0.72 -0.6 4,569,749 - DZA65_RS21135 0.72 -0.6 4,569,784 + -0.4 4,569,785 - DZA65_RS21135 0.75 -0.9 4,569,884 + DZA65_RS21135 0.83 +0.2 4,569,885 - DZA65_RS21135 0.83 -0.2 4,569,885 - DZA65_RS21135 0.83 -0.0 4,569,885 - DZA65_RS21135 0.83 -0.5 4,569,885 - DZA65_RS21135 0.83 -0.3 4,569,885 - DZA65_RS21135 0.83 +0.6
Or see this region's nucleotide sequence