Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19065 and DZA65_RS19070 are separated by 31 nucleotides DZA65_RS19070 and DZA65_RS19075 are separated by 84 nucleotides DZA65_RS19075 and DZA65_RS19080 are separated by 69 nucleotides DZA65_RS19080 and DZA65_RS19085 are separated by 97 nucleotides DZA65_RS19085 and DZA65_RS19090 are separated by 27 nucleotides
DZA65_RS19065: DZA65_RS19065 - cell division protein ZapD, at 4,121,737 to 4,122,486
_RS19065
DZA65_RS19070: DZA65_RS19070 - DNA gyrase inhibitor YacG, at 4,122,518 to 4,122,715
_RS19070
DZA65_RS19075: DZA65_RS19075 - 8-oxo-dGTP diphosphatase MutT, at 4,122,800 to 4,123,201
_RS19075
DZA65_RS19080: DZA65_RS19080 - preprotein translocase subunit SecA, at 4,123,271 to 4,125,964
_RS19080
DZA65_RS19085: DZA65_RS19085 - secA regulator SecM, at 4,126,062 to 4,126,562
_RS19085
DZA65_RS19090: DZA65_RS19090 - DUF721 domain-containing protein, at 4,126,590 to 4,127,111
_RS19090
Position (kb)
4123
4124
4125
4126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4122.316 kb on + strand, within DZA65_RS19065 at 4122.316 kb on + strand, within DZA65_RS19065 at 4122.317 kb on - strand, within DZA65_RS19065 at 4122.317 kb on - strand, within DZA65_RS19065 at 4122.324 kb on + strand, within DZA65_RS19065 at 4122.325 kb on - strand, within DZA65_RS19065 at 4122.325 kb on - strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.379 kb on + strand, within DZA65_RS19065 at 4122.390 kb on - strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.400 kb on - strand, within DZA65_RS19065 at 4122.400 kb on - strand, within DZA65_RS19065 at 4122.484 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.540 kb on + strand, within DZA65_RS19070 at 4122.541 kb on - strand, within DZA65_RS19070 at 4122.541 kb on - strand, within DZA65_RS19070 at 4122.550 kb on + strand, within DZA65_RS19070 at 4122.583 kb on + strand, within DZA65_RS19070 at 4122.583 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.677 kb on - strand, within DZA65_RS19070 at 4122.677 kb on - strand, within DZA65_RS19070 at 4122.713 kb on + strand at 4122.714 kb on - strand at 4122.731 kb on + strand at 4122.732 kb on - strand at 4122.749 kb on + strand at 4122.766 kb on + strand at 4122.767 kb on - strand at 4122.767 kb on - strand at 4122.772 kb on + strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.784 kb on + strand at 4122.784 kb on + strand at 4122.785 kb on - strand at 4122.785 kb on - strand at 4122.797 kb on + strand at 4122.797 kb on + strand at 4122.797 kb on + strand at 4122.798 kb on - strand at 4122.798 kb on - strand at 4122.798 kb on - strand at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.112 kb on - strand at 4123.112 kb on - strand, within DZA65_RS19075 at 4123.112 kb on - strand, within DZA65_RS19075 at 4123.168 kb on + strand at 4123.184 kb on + strand at 4123.202 kb on + strand at 4123.243 kb on + strand at 4123.243 kb on + strand at 4123.251 kb on - strand at 4123.258 kb on + strand at 4123.273 kb on - strand at 4123.273 kb on - strand at 4123.484 kb on - strand at 4126.228 kb on - strand, within DZA65_RS19085 at 4126.391 kb on - strand at 4126.391 kb on - strand, within DZA65_RS19085 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.959 kb on - strand, within DZA65_RS19090
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,122,316 + DZA65_RS19065 0.77 -1.3 4,122,316 + DZA65_RS19065 0.77 +0.1 4,122,317 - DZA65_RS19065 0.77 +1.2 4,122,317 - DZA65_RS19065 0.77 -0.8 4,122,324 + DZA65_RS19065 0.78 -1.0 4,122,325 - DZA65_RS19065 0.78 -0.9 4,122,325 - DZA65_RS19065 0.78 +0.3 4,122,347 + DZA65_RS19065 0.81 -0.5 4,122,347 + DZA65_RS19065 0.81 -0.9 4,122,347 + DZA65_RS19065 0.81 +0.6 4,122,347 + DZA65_RS19065 0.81 -0.6 4,122,347 + DZA65_RS19065 0.81 +0.5 4,122,347 + DZA65_RS19065 0.81 +0.8 4,122,347 + DZA65_RS19065 0.81 -1.2 4,122,347 + DZA65_RS19065 0.81 -0.3 4,122,348 - DZA65_RS19065 0.81 -0.1 4,122,348 - DZA65_RS19065 0.81 +0.7 4,122,348 - DZA65_RS19065 0.81 +1.2 4,122,348 - DZA65_RS19065 0.81 -0.3 4,122,348 - DZA65_RS19065 0.81 +0.1 4,122,379 + DZA65_RS19065 0.86 +0.4 4,122,390 - DZA65_RS19065 0.87 -0.9 4,122,399 + DZA65_RS19065 0.88 +0.1 4,122,399 + DZA65_RS19065 0.88 -1.6 4,122,399 + DZA65_RS19065 0.88 -0.5 4,122,399 + DZA65_RS19065 0.88 -0.1 4,122,400 - DZA65_RS19065 0.88 +0.0 4,122,400 - DZA65_RS19065 0.88 -0.6 4,122,484 + -1.2 4,122,514 + +0.8 4,122,514 + -0.8 4,122,514 + -0.4 4,122,514 + -1.1 4,122,514 + +0.0 4,122,515 - +0.8 4,122,515 - +0.7 4,122,515 - +1.1 4,122,515 - -1.3 4,122,515 - +0.1 4,122,515 - +0.1 4,122,540 + DZA65_RS19070 0.11 -0.6 4,122,541 - DZA65_RS19070 0.12 -1.8 4,122,541 - DZA65_RS19070 0.12 +0.2 4,122,550 + DZA65_RS19070 0.16 -0.6 4,122,583 + DZA65_RS19070 0.33 -0.8 4,122,583 + DZA65_RS19070 0.33 -0.0 4,122,676 + DZA65_RS19070 0.80 -0.2 4,122,676 + DZA65_RS19070 0.80 -0.8 4,122,676 + DZA65_RS19070 0.80 -0.0 4,122,676 + DZA65_RS19070 0.80 -1.2 4,122,676 + DZA65_RS19070 0.80 +0.2 4,122,676 + DZA65_RS19070 0.80 +0.7 4,122,676 + DZA65_RS19070 0.80 -0.0 4,122,677 - DZA65_RS19070 0.80 -0.9 4,122,677 - DZA65_RS19070 0.80 -0.2 4,122,713 + +0.2 4,122,714 - -0.2 4,122,731 + +0.2 4,122,732 - -0.2 4,122,749 + -0.3 4,122,766 + -0.5 4,122,767 - -0.5 4,122,767 - +0.2 4,122,772 + +0.3 4,122,773 - -0.6 4,122,773 - -1.8 4,122,773 - -1.9 4,122,773 - -0.6 4,122,773 - +0.1 4,122,784 + +0.1 4,122,784 + -0.5 4,122,785 - +0.5 4,122,785 - -0.6 4,122,797 + -2.6 4,122,797 + -1.0 4,122,797 + -0.6 4,122,798 - -0.8 4,122,798 - +1.4 4,122,798 - +0.3 4,122,956 - DZA65_RS19075 0.39 -0.4 4,122,956 - DZA65_RS19075 0.39 -0.2 4,122,956 - DZA65_RS19075 0.39 +1.0 4,122,956 - DZA65_RS19075 0.39 -0.8 4,123,111 + DZA65_RS19075 0.77 +0.2 4,123,111 + DZA65_RS19075 0.77 +0.8 4,123,111 + DZA65_RS19075 0.77 -0.9 4,123,111 + DZA65_RS19075 0.77 +0.0 4,123,112 - +1.4 4,123,112 - DZA65_RS19075 0.78 -0.3 4,123,112 - DZA65_RS19075 0.78 +0.2 4,123,168 + +0.5 4,123,184 + +0.5 4,123,202 + -0.5 4,123,243 + -0.6 4,123,243 + -0.1 4,123,251 - -0.2 4,123,258 + -1.0 4,123,273 - -0.1 4,123,273 - -0.8 4,123,484 - -1.1 4,126,228 - DZA65_RS19085 0.33 -1.8 4,126,391 - +2.1 4,126,391 - DZA65_RS19085 0.66 -1.0 4,126,940 + DZA65_RS19090 0.67 -1.0 4,126,940 + DZA65_RS19090 0.67 -0.2 4,126,940 + DZA65_RS19090 0.67 +1.4 4,126,958 + DZA65_RS19090 0.70 -0.3 4,126,958 + DZA65_RS19090 0.70 -0.6 4,126,959 - DZA65_RS19090 0.71 +0.2
Or see this region's nucleotide sequence