Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03200 and HGI48_RS03205 are separated by 105 nucleotides HGI48_RS03205 and HGI48_RS03210 are separated by 8 nucleotides HGI48_RS03210 and HGI48_RS03215 are separated by 298 nucleotides HGI48_RS03215 and HGI48_RS03220 are separated by 54 nucleotides HGI48_RS03220 and HGI48_RS03225 are separated by 59 nucleotides
HGI48_RS03200: HGI48_RS03200 - ATP-dependent zinc metalloprotease FtsH, at 675,830 to 677,782
_RS03200
HGI48_RS03205: HGI48_RS03205 - dihydropteroate synthase, at 677,888 to 678,721
_RS03205
HGI48_RS03210: HGI48_RS03210 - phosphoglucosamine mutase, at 678,730 to 680,067
_RS03210
HGI48_RS03215: HGI48_RS03215 - preprotein translocase subunit SecG, at 680,366 to 680,701
_RS03215
HGI48_RS03220: HGI48_RS03220 - tRNA-Leu, at 680,756 to 680,842
_RS03220
HGI48_RS03225: HGI48_RS03225 - tRNA-Met, at 680,902 to 680,978
_RS03225
Position (kb) 678
679
680
681 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 677.734 kb on + strand at 677.734 kb on + strand at 677.735 kb on - strand at 677.752 kb on + strand at 677.752 kb on + strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.859 kb on - strand at 677.875 kb on + strand at 677.875 kb on + strand at 678.348 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.468 kb on + strand, within HGI48_RS03205 at 678.475 kb on + strand, within HGI48_RS03205 at 678.476 kb on - strand, within HGI48_RS03205 at 678.684 kb on + strand at 678.684 kb on + strand at 678.684 kb on + strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.719 kb on + strand at 678.729 kb on + strand at 680.077 kb on + strand at 680.078 kb on - strand at 680.078 kb on - strand at 680.143 kb on - strand at 680.143 kb on - strand at 680.143 kb on - strand at 680.247 kb on - strand at 680.423 kb on - strand, within HGI48_RS03215 at 680.695 kb on + strand at 680.695 kb on + strand at 680.740 kb on + strand at 680.740 kb on + strand at 680.848 kb on - strand at 680.849 kb on + strand at 680.850 kb on - strand at 680.853 kb on + strand at 680.853 kb on + strand at 680.854 kb on - strand at 680.854 kb on - strand at 680.981 kb on + strand at 680.981 kb on + strand at 681.009 kb on + strand at 681.016 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 677,734 + -0.4 677,734 + -1.4 677,735 - -3.1 677,752 + -0.6 677,752 + -0.2 677,753 - -2.0 677,753 - -0.4 677,753 - -1.5 677,753 - -1.9 677,753 - +0.1 677,753 - -1.2 677,753 - +0.1 677,780 + +0.1 677,780 + -0.7 677,780 + -1.0 677,780 + +0.7 677,780 + +0.1 677,780 + +0.1 677,780 + -0.7 677,780 + +0.1 677,780 + -0.7 677,781 - -0.6 677,781 - -0.5 677,781 - -0.4 677,781 - -0.8 677,781 - -1.6 677,781 - +1.1 677,859 - +0.6 677,875 + +1.1 677,875 + +1.6 678,348 - HGI48_RS03205 0.55 -1.2 678,392 - HGI48_RS03205 0.60 -0.9 678,392 - HGI48_RS03205 0.60 +0.7 678,468 + HGI48_RS03205 0.70 +0.4 678,475 + HGI48_RS03205 0.70 -0.9 678,476 - HGI48_RS03205 0.71 +0.1 678,684 + -2.6 678,684 + -3.8 678,684 + -1.7 678,685 - -1.2 678,685 - -2.8 678,685 - -0.6 678,685 - -0.3 678,685 - -2.4 678,685 - -1.4 678,719 + -0.8 678,729 + -1.9 680,077 + -1.2 680,078 - -0.4 680,078 - -1.4 680,143 - -1.5 680,143 - -1.4 680,143 - -1.5 680,247 - -0.7 680,423 - HGI48_RS03215 0.17 -1.3 680,695 + +0.7 680,695 + -1.7 680,740 + -0.1 680,740 + -1.4 680,848 - -1.7 680,849 + +3.5 680,850 - -2.3 680,853 + -1.0 680,853 + -0.0 680,854 - -0.7 680,854 - -0.4 680,981 + -0.6 680,981 + -2.0 681,009 + -0.9 681,016 + +0.1 681,067 + -1.4 681,067 + -2.3 681,067 + -1.3 681,067 + -0.9
Or see this region's nucleotide sequence