Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03320 and HGI48_RS03325 are separated by 70 nucleotides HGI48_RS03325 and HGI48_RS03330 are separated by 73 nucleotides HGI48_RS03330 and HGI48_RS03335 overlap by 32 nucleotides
HGI48_RS03320: HGI48_RS03320 - phosphopentomutase, at 700,438 to 701,661
_RS03320
HGI48_RS03325: HGI48_RS03325 - purine-nucleoside phosphorylase, at 701,732 to 702,457
_RS03325
HGI48_RS03330: HGI48_RS03330 - ribosomal protein S18-alanine N-acetyltransferase, at 702,531 to 702,974
_RS03330
HGI48_RS03335: HGI48_RS03335 - DNA polymerase III subunit psi, at 702,943 to 703,362
_RS03335
Position (kb)
701
702
703 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 700.943 kb on - strand, within HGI48_RS03320 at 701.053 kb on + strand at 701.228 kb on - strand, within HGI48_RS03320 at 701.813 kb on + strand, within HGI48_RS03325 at 701.814 kb on - strand, within HGI48_RS03325 at 701.867 kb on - strand, within HGI48_RS03325 at 701.888 kb on + strand, within HGI48_RS03325 at 701.889 kb on - strand, within HGI48_RS03325 at 701.889 kb on - strand, within HGI48_RS03325 at 701.949 kb on - strand, within HGI48_RS03325 at 702.095 kb on - strand at 702.212 kb on + strand, within HGI48_RS03325 at 702.212 kb on + strand at 702.290 kb on + strand, within HGI48_RS03325 at 702.291 kb on - strand, within HGI48_RS03325 at 702.349 kb on + strand, within HGI48_RS03325 at 702.466 kb on + strand at 702.467 kb on - strand at 702.469 kb on + strand at 702.470 kb on - strand at 702.470 kb on - strand at 702.470 kb on - strand at 702.470 kb on - strand at 702.506 kb on + strand at 702.506 kb on + strand at 702.506 kb on + strand at 702.507 kb on - strand at 702.507 kb on - strand at 702.587 kb on - strand, within HGI48_RS03330 at 702.589 kb on + strand, within HGI48_RS03330 at 702.590 kb on - strand, within HGI48_RS03330 at 702.590 kb on - strand, within HGI48_RS03330 at 702.632 kb on - strand, within HGI48_RS03330 at 702.675 kb on + strand, within HGI48_RS03330 at 702.675 kb on + strand, within HGI48_RS03330 at 702.676 kb on - strand, within HGI48_RS03330 at 702.676 kb on - strand, within HGI48_RS03330 at 702.676 kb on - strand, within HGI48_RS03330 at 702.782 kb on + strand at 702.782 kb on + strand, within HGI48_RS03330 at 702.783 kb on - strand, within HGI48_RS03330 at 702.783 kb on - strand, within HGI48_RS03330 at 702.795 kb on + strand, within HGI48_RS03330 at 702.796 kb on - strand, within HGI48_RS03330 at 702.798 kb on + strand, within HGI48_RS03330 at 702.798 kb on + strand at 702.798 kb on + strand, within HGI48_RS03330 at 702.799 kb on - strand, within HGI48_RS03330 at 702.799 kb on - strand, within HGI48_RS03330 at 702.799 kb on - strand, within HGI48_RS03330 at 702.814 kb on + strand, within HGI48_RS03330 at 702.814 kb on + strand, within HGI48_RS03330 at 702.815 kb on - strand, within HGI48_RS03330 at 702.913 kb on - strand, within HGI48_RS03330 at 703.368 kb on - strand at 703.401 kb on + strand at 703.420 kb on + strand at 703.421 kb on - strand at 703.421 kb on - strand at 703.421 kb on - strand at 703.436 kb on + strand at 703.436 kb on + strand at 703.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 700,943 - HGI48_RS03320 0.41 +0.9 701,053 + +3.8 701,228 - HGI48_RS03320 0.65 -1.9 701,813 + HGI48_RS03325 0.11 -1.1 701,814 - HGI48_RS03325 0.11 -0.9 701,867 - HGI48_RS03325 0.19 -0.9 701,888 + HGI48_RS03325 0.21 -0.5 701,889 - HGI48_RS03325 0.22 -1.7 701,889 - HGI48_RS03325 0.22 -0.4 701,949 - HGI48_RS03325 0.30 -0.3 702,095 - -0.1 702,212 + HGI48_RS03325 0.66 -0.7 702,212 + -0.7 702,290 + HGI48_RS03325 0.77 -0.9 702,291 - HGI48_RS03325 0.77 -2.4 702,349 + HGI48_RS03325 0.85 +1.2 702,466 + -0.2 702,467 - +0.6 702,469 + -1.1 702,470 - -0.9 702,470 - -2.3 702,470 - +0.5 702,470 - -1.7 702,506 + -0.4 702,506 + +0.9 702,506 + -0.2 702,507 - -1.9 702,507 - -1.4 702,587 - HGI48_RS03330 0.13 +0.4 702,589 + HGI48_RS03330 0.13 +0.1 702,590 - HGI48_RS03330 0.13 -1.1 702,590 - HGI48_RS03330 0.13 -0.8 702,632 - HGI48_RS03330 0.23 -1.7 702,675 + HGI48_RS03330 0.32 -0.2 702,675 + HGI48_RS03330 0.32 +0.6 702,676 - HGI48_RS03330 0.33 -1.2 702,676 - HGI48_RS03330 0.33 +7.0 702,676 - HGI48_RS03330 0.33 -0.1 702,782 + -0.1 702,782 + HGI48_RS03330 0.57 -1.7 702,783 - HGI48_RS03330 0.57 -0.3 702,783 - HGI48_RS03330 0.57 +1.7 702,795 + HGI48_RS03330 0.59 +0.5 702,796 - HGI48_RS03330 0.60 -1.9 702,798 + HGI48_RS03330 0.60 +0.5 702,798 + +0.3 702,798 + HGI48_RS03330 0.60 -0.9 702,799 - HGI48_RS03330 0.60 -1.0 702,799 - HGI48_RS03330 0.60 -0.7 702,799 - HGI48_RS03330 0.60 +0.8 702,814 + HGI48_RS03330 0.64 +1.6 702,814 + HGI48_RS03330 0.64 -0.4 702,815 - HGI48_RS03330 0.64 +1.5 702,913 - HGI48_RS03330 0.86 -0.6 703,368 - -4.5 703,401 + -0.9 703,420 + +0.3 703,421 - +1.9 703,421 - -0.7 703,421 - -0.2 703,436 + -2.3 703,436 + -0.1 703,437 - -1.1
Or see this region's nucleotide sequence