Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS02350 and HGI48_RS02355 are separated by 39 nucleotides HGI48_RS02355 and HGI48_RS02360 are separated by 234 nucleotides HGI48_RS02360 and HGI48_RS02370 are separated by 338 nucleotides HGI48_RS02370 and HGI48_RS02375 are separated by 13 nucleotides
HGI48_RS02350: HGI48_RS02350 - Xaa-Pro aminopeptidase, at 510,720 to 512,048
_RS02350
HGI48_RS02355: HGI48_RS02355 - YecA family protein, at 512,088 to 512,672
_RS02355
HGI48_RS02360: HGI48_RS02360 - cell division protein ZapA, at 512,907 to 513,236
_RS02360
HGI48_RS02370: HGI48_RS02370 - 5-formyltetrahydrofolate cyclo-ligase, at 513,575 to 514,189
_RS02370
HGI48_RS02375: HGI48_RS02375 - multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR, at 514,203 to 515,459
_RS02375
Position (kb) 512
513
514 Strain fitness (log2 ratio) -2
-1
0
1
2 at 511.950 kb on - strand at 512.033 kb on - strand at 512.172 kb on + strand, within HGI48_RS02355 at 512.172 kb on + strand, within HGI48_RS02355 at 512.172 kb on + strand, within HGI48_RS02355 at 512.172 kb on + strand, within HGI48_RS02355 at 512.173 kb on - strand, within HGI48_RS02355 at 512.173 kb on - strand, within HGI48_RS02355 at 512.176 kb on + strand at 512.176 kb on + strand, within HGI48_RS02355 at 512.176 kb on + strand, within HGI48_RS02355 at 512.176 kb on + strand, within HGI48_RS02355 at 512.177 kb on - strand, within HGI48_RS02355 at 512.177 kb on - strand, within HGI48_RS02355 at 512.177 kb on - strand, within HGI48_RS02355 at 512.177 kb on - strand, within HGI48_RS02355 at 512.257 kb on - strand, within HGI48_RS02355 at 512.257 kb on - strand, within HGI48_RS02355 at 512.257 kb on - strand, within HGI48_RS02355 at 512.373 kb on + strand at 512.373 kb on + strand, within HGI48_RS02355 at 512.459 kb on - strand, within HGI48_RS02355 at 512.574 kb on + strand, within HGI48_RS02355 at 512.626 kb on - strand at 512.657 kb on + strand at 512.657 kb on + strand at 512.657 kb on + strand at 512.658 kb on - strand at 512.658 kb on - strand at 512.658 kb on - strand at 512.664 kb on + strand at 512.667 kb on - strand at 512.707 kb on - strand at 512.711 kb on + strand at 512.730 kb on + strand at 512.731 kb on - strand at 512.731 kb on - strand at 512.732 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.771 kb on - strand at 512.771 kb on - strand at 512.777 kb on + strand at 512.777 kb on + strand at 512.777 kb on + strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.789 kb on - strand at 512.789 kb on - strand at 512.789 kb on - strand at 512.795 kb on + strand at 512.795 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.815 kb on + strand at 512.815 kb on + strand at 512.815 kb on + strand at 512.820 kb on + strand at 513.235 kb on - strand at 513.296 kb on + strand at 513.296 kb on + strand at 513.296 kb on + strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.301 kb on + strand at 513.302 kb on - strand at 513.302 kb on - strand at 513.381 kb on + strand at 513.381 kb on + strand at 513.382 kb on - strand at 513.382 kb on - strand at 513.382 kb on - strand at 513.428 kb on - strand at 513.531 kb on + strand at 513.531 kb on + strand at 513.531 kb on + strand at 513.544 kb on + strand at 513.561 kb on - strand at 513.621 kb on + strand at 513.830 kb on + strand, within HGI48_RS02370 at 513.830 kb on + strand, within HGI48_RS02370 at 513.831 kb on - strand, within HGI48_RS02370 at 513.831 kb on - strand, within HGI48_RS02370 at 513.876 kb on + strand, within HGI48_RS02370 at 513.877 kb on - strand, within HGI48_RS02370 at 513.877 kb on - strand, within HGI48_RS02370 at 514.041 kb on - strand, within HGI48_RS02370 at 514.041 kb on - strand, within HGI48_RS02370 at 514.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 511,950 - -1.2 512,033 - +1.4 512,172 + HGI48_RS02355 0.14 +0.5 512,172 + HGI48_RS02355 0.14 +1.1 512,172 + HGI48_RS02355 0.14 +1.8 512,172 + HGI48_RS02355 0.14 -0.5 512,173 - HGI48_RS02355 0.15 -1.4 512,173 - HGI48_RS02355 0.15 -0.0 512,176 + +1.0 512,176 + HGI48_RS02355 0.15 +0.2 512,176 + HGI48_RS02355 0.15 -0.6 512,176 + HGI48_RS02355 0.15 +0.5 512,177 - HGI48_RS02355 0.15 +0.5 512,177 - HGI48_RS02355 0.15 -1.5 512,177 - HGI48_RS02355 0.15 +1.0 512,177 - HGI48_RS02355 0.15 -1.0 512,257 - HGI48_RS02355 0.29 -0.8 512,257 - HGI48_RS02355 0.29 -1.4 512,257 - HGI48_RS02355 0.29 +0.2 512,373 + -0.6 512,373 + HGI48_RS02355 0.49 -1.0 512,459 - HGI48_RS02355 0.63 -0.5 512,574 + HGI48_RS02355 0.83 +0.1 512,626 - -0.6 512,657 + -0.4 512,657 + -1.0 512,657 + -0.7 512,658 - -0.6 512,658 - -0.0 512,658 - -0.7 512,664 + +2.3 512,667 - +1.2 512,707 - -0.6 512,711 + -0.6 512,730 + -1.8 512,731 - +1.0 512,731 - -1.6 512,732 + -2.5 512,770 + -1.0 512,770 + -1.0 512,770 + -0.0 512,770 + +1.5 512,770 + -1.6 512,771 - +1.2 512,771 - +1.6 512,777 + -0.0 512,777 + -0.6 512,777 + +1.0 512,778 - -1.0 512,778 - +0.3 512,778 - -1.6 512,778 - +0.4 512,788 + -1.0 512,788 + +0.4 512,788 + +0.2 512,788 + -0.0 512,788 + +0.3 512,788 + -1.0 512,789 - -0.0 512,789 - -1.6 512,789 - -2.4 512,795 + +0.2 512,795 + -0.7 512,813 + -1.7 512,813 + +1.2 512,813 + +0.6 512,813 + -0.5 512,813 + -1.0 512,815 + -0.8 512,815 + +0.1 512,815 + +0.2 512,820 + -0.7 513,235 - +0.8 513,296 + -2.0 513,296 + +0.2 513,296 + +0.8 513,297 - +0.3 513,297 - +0.5 513,297 - +1.7 513,297 - -1.8 513,297 - +2.7 513,297 - +1.4 513,297 - +0.8 513,301 + -0.6 513,302 - +2.5 513,302 - -0.0 513,381 + -0.3 513,381 + +1.0 513,382 - +2.0 513,382 - -0.4 513,382 - -1.5 513,428 - +0.7 513,531 + +0.5 513,531 + -0.5 513,531 + +1.0 513,544 + +1.4 513,561 - -1.0 513,621 + +1.5 513,830 + HGI48_RS02370 0.41 -0.4 513,830 + HGI48_RS02370 0.41 -0.0 513,831 - HGI48_RS02370 0.42 +1.0 513,831 - HGI48_RS02370 0.42 -1.0 513,876 + HGI48_RS02370 0.49 -1.0 513,877 - HGI48_RS02370 0.49 +0.6 513,877 - HGI48_RS02370 0.49 -1.4 514,041 - HGI48_RS02370 0.76 +0.1 514,041 - HGI48_RS02370 0.76 -0.5 514,193 - -1.0
Or see this region's nucleotide sequence