Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01175 and HGI48_RS01180 are separated by 105 nucleotides HGI48_RS01180 and HGI48_RS01185 are separated by 55 nucleotides HGI48_RS01185 and HGI48_RS01190 overlap by 1 nucleotides HGI48_RS01190 and HGI48_RS01195 are separated by 91 nucleotides
HGI48_RS01175: HGI48_RS01175 - sigma D regulator, at 263,985 to 264,434
_RS01175
HGI48_RS01180: HGI48_RS01180 - NAD(+) diphosphatase, at 264,540 to 265,313
_RS01180
HGI48_RS01185: HGI48_RS01185 - uroporphyrinogen decarboxylase, at 265,369 to 266,442
_RS01185
HGI48_RS01190: HGI48_RS01190 - deoxyribonuclease V, at 266,442 to 267,116
_RS01190
HGI48_RS01195: HGI48_RS01195 - YjaG family protein, at 267,208 to 267,798
_RS01195
Position (kb) 265
266
267 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.420 kb on + strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.454 kb on + strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.475 kb on + strand at 264.481 kb on + strand at 264.482 kb on - strand at 264.482 kb on - strand at 264.485 kb on + strand at 264.485 kb on + strand at 264.486 kb on - strand at 264.493 kb on + strand at 264.494 kb on - strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.518 kb on + strand at 264.519 kb on - strand at 264.533 kb on - strand at 264.585 kb on + strand at 264.585 kb on + strand at 264.586 kb on - strand at 264.623 kb on - strand at 264.719 kb on + strand, within HGI48_RS01180 at 264.719 kb on + strand at 264.814 kb on - strand, within HGI48_RS01180 at 264.814 kb on - strand, within HGI48_RS01180 at 264.875 kb on - strand, within HGI48_RS01180 at 264.896 kb on + strand, within HGI48_RS01180 at 264.897 kb on - strand, within HGI48_RS01180 at 264.907 kb on - strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.080 kb on - strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.161 kb on + strand, within HGI48_RS01180 at 265.221 kb on + strand, within HGI48_RS01180 at 265.222 kb on - strand, within HGI48_RS01180 at 265.286 kb on - strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.355 kb on - strand at 266.422 kb on + strand at 266.448 kb on - strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.508 kb on - strand at 266.508 kb on - strand at 266.625 kb on + strand, within HGI48_RS01190 at 266.626 kb on - strand at 266.640 kb on - strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 267.114 kb on + strand at 267.114 kb on + strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.140 kb on - strand at 267.151 kb on + strand at 267.151 kb on + strand at 267.151 kb on + strand at 267.153 kb on + strand at 267.176 kb on + strand at 267.188 kb on + strand at 267.188 kb on + strand at 267.188 kb on + strand at 267.191 kb on + strand at 267.191 kb on + strand at 267.191 kb on + strand at 267.192 kb on - strand at 267.192 kb on - strand at 267.202 kb on + strand at 267.203 kb on - strand at 267.213 kb on - strand at 267.291 kb on + strand, within HGI48_RS01195 at 267.291 kb on + strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.295 kb on + strand, within HGI48_RS01195 at 267.304 kb on + strand, within HGI48_RS01195 at 267.304 kb on + strand, within HGI48_RS01195 at 267.305 kb on - strand, within HGI48_RS01195 at 267.305 kb on - strand, within HGI48_RS01195 at 267.308 kb on - strand at 267.358 kb on - strand, within HGI48_RS01195
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 264,388 + HGI48_RS01175 0.90 -1.5 264,388 + HGI48_RS01175 0.90 -0.7 264,388 + HGI48_RS01175 0.90 +1.5 264,389 - HGI48_RS01175 0.90 +0.2 264,389 - HGI48_RS01175 0.90 +0.0 264,389 - HGI48_RS01175 0.90 +1.3 264,389 - HGI48_RS01175 0.90 -1.0 264,420 + -0.7 264,421 - +0.0 264,421 - -0.2 264,421 - -0.4 264,454 + -0.7 264,455 - +2.0 264,455 - +0.0 264,455 - -1.0 264,475 + +0.8 264,481 + -1.3 264,482 - -1.1 264,482 - +0.5 264,485 + +1.0 264,485 + +0.0 264,486 - +1.8 264,493 + -0.1 264,494 - +0.0 264,499 + -0.7 264,499 + -1.5 264,499 + +1.6 264,518 + -0.2 264,519 - -0.7 264,533 - -0.5 264,585 + -3.1 264,585 + +0.6 264,586 - -1.3 264,623 - +2.4 264,719 + HGI48_RS01180 0.23 -1.5 264,719 + -0.5 264,814 - HGI48_RS01180 0.35 -0.2 264,814 - HGI48_RS01180 0.35 +0.5 264,875 - HGI48_RS01180 0.43 -2.0 264,896 + HGI48_RS01180 0.46 -0.2 264,897 - HGI48_RS01180 0.46 -0.8 264,907 - HGI48_RS01180 0.47 -0.5 264,999 + HGI48_RS01180 0.59 +0.2 264,999 + HGI48_RS01180 0.59 -1.7 264,999 + HGI48_RS01180 0.59 -1.5 264,999 + HGI48_RS01180 0.59 +0.3 264,999 + HGI48_RS01180 0.59 -0.1 264,999 + HGI48_RS01180 0.59 +0.0 264,999 + +0.5 264,999 + HGI48_RS01180 0.59 -1.0 264,999 + +0.0 265,000 - HGI48_RS01180 0.59 -1.8 265,000 - HGI48_RS01180 0.59 +0.7 265,000 - -0.5 265,000 - HGI48_RS01180 0.59 -1.8 265,000 - HGI48_RS01180 0.59 +0.3 265,000 - HGI48_RS01180 0.59 +0.3 265,080 - HGI48_RS01180 0.70 +2.1 265,101 + HGI48_RS01180 0.72 -3.1 265,101 + HGI48_RS01180 0.72 -0.5 265,101 + HGI48_RS01180 0.72 -0.8 265,102 - HGI48_RS01180 0.73 -0.8 265,102 - HGI48_RS01180 0.73 -0.3 265,102 - HGI48_RS01180 0.73 -1.0 265,102 - HGI48_RS01180 0.73 +0.6 265,161 + HGI48_RS01180 0.80 -0.3 265,221 + HGI48_RS01180 0.88 -0.4 265,222 - HGI48_RS01180 0.88 -0.3 265,286 - +1.2 265,288 + -0.2 265,288 + -0.5 265,288 + -0.4 265,289 - +0.0 265,289 - +1.5 265,289 - +1.0 265,355 - -2.5 266,422 + -0.1 266,448 - +0.6 266,507 + +0.0 266,507 + +0.0 266,507 + -1.0 266,507 + -0.1 266,508 - -1.0 266,508 - +0.6 266,625 + HGI48_RS01190 0.27 -1.3 266,626 - +2.3 266,640 - HGI48_RS01190 0.29 +1.0 266,658 + HGI48_RS01190 0.32 +0.3 266,658 + HGI48_RS01190 0.32 -0.5 266,658 + HGI48_RS01190 0.32 -0.5 266,658 + HGI48_RS01190 0.32 +0.0 266,658 + HGI48_RS01190 0.32 +0.2 266,658 + HGI48_RS01190 0.32 +0.3 266,658 + HGI48_RS01190 0.32 +0.6 266,658 + HGI48_RS01190 0.32 -0.4 266,658 + HGI48_RS01190 0.32 +0.0 266,658 + HGI48_RS01190 0.32 -1.3 266,658 + HGI48_RS01190 0.32 -0.4 266,658 + HGI48_RS01190 0.32 -0.4 266,658 + HGI48_RS01190 0.32 -1.1 266,658 + HGI48_RS01190 0.32 +0.0 266,658 + HGI48_RS01190 0.32 -1.0 266,658 + +0.0 266,658 + HGI48_RS01190 0.32 +0.7 266,658 + HGI48_RS01190 0.32 -0.3 266,658 + HGI48_RS01190 0.32 -0.4 266,658 + HGI48_RS01190 0.32 +0.2 266,658 + HGI48_RS01190 0.32 -1.0 266,659 - HGI48_RS01190 0.32 +1.2 266,659 - HGI48_RS01190 0.32 +0.2 266,659 - HGI48_RS01190 0.32 -0.8 266,659 - HGI48_RS01190 0.32 -0.4 266,659 - HGI48_RS01190 0.32 +0.0 266,659 - +1.0 266,659 - HGI48_RS01190 0.32 -0.1 266,659 - HGI48_RS01190 0.32 -1.8 266,659 - +1.0 266,659 - HGI48_RS01190 0.32 +0.9 266,659 - HGI48_RS01190 0.32 +0.6 266,659 - HGI48_RS01190 0.32 +0.4 266,659 - HGI48_RS01190 0.32 +0.6 266,659 - HGI48_RS01190 0.32 -0.4 266,659 - HGI48_RS01190 0.32 -1.4 266,660 + HGI48_RS01190 0.32 -1.5 266,660 + HGI48_RS01190 0.32 -1.3 266,660 + HGI48_RS01190 0.32 +0.9 266,660 + HGI48_RS01190 0.32 +0.1 266,661 - HGI48_RS01190 0.32 -0.1 266,661 - HGI48_RS01190 0.32 -0.3 266,661 - HGI48_RS01190 0.32 +1.0 266,661 - HGI48_RS01190 0.32 +0.6 267,114 + -0.1 267,114 + +1.0 267,115 - -1.8 267,115 - -0.7 267,115 - -1.3 267,115 - -2.0 267,140 - +0.6 267,151 + -0.8 267,151 + -0.1 267,151 + +1.6 267,153 + +0.8 267,176 + +2.4 267,188 + -0.3 267,188 + -2.4 267,188 + +0.5 267,191 + +0.3 267,191 + +1.8 267,191 + -0.5 267,192 - -0.4 267,192 - -1.0 267,202 + +0.2 267,203 - -0.3 267,213 - +0.0 267,291 + HGI48_RS01195 0.14 -0.1 267,291 + HGI48_RS01195 0.14 +1.2 267,292 - HGI48_RS01195 0.14 -1.3 267,292 - HGI48_RS01195 0.14 -2.0 267,292 - HGI48_RS01195 0.14 -0.5 267,292 - HGI48_RS01195 0.14 +0.6 267,292 - HGI48_RS01195 0.14 -2.1 267,292 - HGI48_RS01195 0.14 -0.5 267,292 - HGI48_RS01195 0.14 -1.5 267,292 - HGI48_RS01195 0.14 +0.5 267,292 - HGI48_RS01195 0.14 +0.6 267,292 - HGI48_RS01195 0.14 -1.0 267,295 + HGI48_RS01195 0.15 -1.5 267,304 + HGI48_RS01195 0.16 +0.5 267,304 + HGI48_RS01195 0.16 +0.0 267,305 - HGI48_RS01195 0.16 +0.8 267,305 - HGI48_RS01195 0.16 -0.4 267,308 - +2.0 267,358 - HGI48_RS01195 0.25 +0.6
Or see this region's nucleotide sequence