Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS02480 and HGI48_RS02485 overlap by 4 nucleotides HGI48_RS02485 and HGI48_RS02490 are separated by 302 nucleotides HGI48_RS02490 and HGI48_RS02495 are separated by 138 nucleotides
HGI48_RS02480: HGI48_RS02480 - hydroxyisourate hydrolase, at 536,468 to 536,800
_RS02480
HGI48_RS02485: HGI48_RS02485 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 536,797 to 537,297
_RS02485
HGI48_RS02490: HGI48_RS02490 - LysR family transcriptional regulator, at 537,600 to 538,538
_RS02490
HGI48_RS02495: HGI48_RS02495 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 538,677 to 539,711
_RS02495
Position (kb) 537
538
539 Strain fitness (log2 ratio) -2
-1
0
1
2 at 536.788 kb on + strand at 536.789 kb on - strand at 536.789 kb on - strand at 536.794 kb on + strand at 536.795 kb on - strand at 536.795 kb on - strand at 536.832 kb on + strand at 537.012 kb on + strand, within HGI48_RS02485 at 537.152 kb on - strand, within HGI48_RS02485 at 537.276 kb on + strand at 537.277 kb on - strand at 537.286 kb on + strand at 537.286 kb on + strand at 537.286 kb on + strand at 537.287 kb on - strand at 537.287 kb on - strand at 537.287 kb on - strand at 537.287 kb on - strand at 537.329 kb on + strand at 537.329 kb on + strand at 537.329 kb on + strand at 537.330 kb on - strand at 537.364 kb on + strand at 537.389 kb on + strand at 537.401 kb on + strand at 537.404 kb on - strand at 537.433 kb on - strand at 537.444 kb on + strand at 537.444 kb on + strand at 537.444 kb on + strand at 537.444 kb on + strand at 537.444 kb on + strand at 537.444 kb on + strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.445 kb on - strand at 537.482 kb on - strand at 537.482 kb on - strand at 537.517 kb on + strand at 537.535 kb on + strand at 537.564 kb on + strand at 537.678 kb on + strand at 537.678 kb on + strand at 537.794 kb on + strand, within HGI48_RS02490 at 537.794 kb on + strand, within HGI48_RS02490 at 537.796 kb on + strand, within HGI48_RS02490 at 537.900 kb on - strand, within HGI48_RS02490 at 537.910 kb on + strand, within HGI48_RS02490 at 537.911 kb on - strand, within HGI48_RS02490 at 537.911 kb on - strand, within HGI48_RS02490 at 537.911 kb on - strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.139 kb on + strand, within HGI48_RS02490 at 538.140 kb on - strand, within HGI48_RS02490 at 538.158 kb on - strand, within HGI48_RS02490 at 538.213 kb on - strand, within HGI48_RS02490 at 538.309 kb on + strand, within HGI48_RS02490 at 538.309 kb on + strand, within HGI48_RS02490 at 538.309 kb on + strand, within HGI48_RS02490 at 538.310 kb on - strand, within HGI48_RS02490 at 538.340 kb on - strand, within HGI48_RS02490 at 538.340 kb on - strand at 538.552 kb on - strand at 538.587 kb on - strand at 538.587 kb on - strand at 538.617 kb on + strand at 538.617 kb on + strand at 538.618 kb on - strand at 538.618 kb on - strand at 538.618 kb on - strand at 538.643 kb on + strand at 538.646 kb on - strand at 538.646 kb on - strand at 538.646 kb on - strand at 538.646 kb on - strand at 538.646 kb on - strand at 538.862 kb on + strand, within HGI48_RS02495 at 538.862 kb on + strand, within HGI48_RS02495 at 538.863 kb on - strand, within HGI48_RS02495 at 538.863 kb on - strand, within HGI48_RS02495 at 539.040 kb on + strand, within HGI48_RS02495 at 539.198 kb on - strand, within HGI48_RS02495 at 539.226 kb on - strand, within HGI48_RS02495 at 539.301 kb on + strand, within HGI48_RS02495 at 539.301 kb on + strand, within HGI48_RS02495 at 539.302 kb on - strand, within HGI48_RS02495 at 539.302 kb on - strand, within HGI48_RS02495 at 539.396 kb on + strand, within HGI48_RS02495
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 536,788 + -2.0 536,789 - -1.7 536,789 - -1.5 536,794 + -0.7 536,795 - -0.1 536,795 - -0.9 536,832 + -1.0 537,012 + HGI48_RS02485 0.43 +1.6 537,152 - HGI48_RS02485 0.71 +0.5 537,276 + +0.4 537,277 - -0.0 537,286 + -0.7 537,286 + +0.3 537,286 + -0.0 537,287 - -0.2 537,287 - -0.2 537,287 - +0.8 537,287 - -1.6 537,329 + +1.2 537,329 + +1.7 537,329 + -0.5 537,330 - +0.8 537,364 + +0.3 537,389 + +0.1 537,401 + -1.5 537,404 - -0.5 537,433 - +1.8 537,444 + -0.5 537,444 + +1.1 537,444 + +0.3 537,444 + +0.1 537,444 + +0.6 537,444 + -1.3 537,445 - +0.8 537,445 - -1.0 537,445 - +0.3 537,445 - -0.7 537,445 - +1.2 537,445 - +2.0 537,445 - -0.5 537,482 - -0.4 537,482 - -0.9 537,517 + -0.2 537,535 + -0.2 537,564 + -0.9 537,678 + -0.2 537,678 + -0.3 537,794 + HGI48_RS02490 0.21 -1.7 537,794 + HGI48_RS02490 0.21 -0.5 537,796 + HGI48_RS02490 0.21 -0.3 537,900 - HGI48_RS02490 0.32 -0.4 537,910 + HGI48_RS02490 0.33 -0.6 537,911 - HGI48_RS02490 0.33 -0.9 537,911 - HGI48_RS02490 0.33 -0.4 537,911 - HGI48_RS02490 0.33 -0.3 538,139 + HGI48_RS02490 0.57 -0.7 538,139 + HGI48_RS02490 0.57 +0.6 538,139 + HGI48_RS02490 0.57 -0.7 538,139 + HGI48_RS02490 0.57 -1.2 538,139 + HGI48_RS02490 0.57 -0.5 538,139 + HGI48_RS02490 0.57 +0.1 538,139 + HGI48_RS02490 0.57 +0.7 538,140 - HGI48_RS02490 0.58 -0.6 538,158 - HGI48_RS02490 0.59 -0.7 538,213 - HGI48_RS02490 0.65 -0.2 538,309 + HGI48_RS02490 0.76 +2.7 538,309 + HGI48_RS02490 0.76 -0.3 538,309 + HGI48_RS02490 0.76 -1.3 538,310 - HGI48_RS02490 0.76 -0.5 538,340 - HGI48_RS02490 0.79 -0.2 538,340 - +2.4 538,552 - -0.7 538,587 - -1.0 538,587 - -1.2 538,617 + +0.3 538,617 + -1.5 538,618 - +0.9 538,618 - -0.9 538,618 - +0.3 538,643 + -0.2 538,646 - +0.8 538,646 - +0.4 538,646 - +0.1 538,646 - -0.4 538,646 - -1.2 538,862 + HGI48_RS02495 0.18 +0.6 538,862 + HGI48_RS02495 0.18 -1.7 538,863 - HGI48_RS02495 0.18 -1.6 538,863 - HGI48_RS02495 0.18 -1.7 539,040 + HGI48_RS02495 0.35 -1.3 539,198 - HGI48_RS02495 0.50 +0.7 539,226 - HGI48_RS02495 0.53 -2.6 539,301 + HGI48_RS02495 0.60 -0.0 539,301 + HGI48_RS02495 0.60 -0.4 539,302 - HGI48_RS02495 0.60 -1.2 539,302 - HGI48_RS02495 0.60 -0.2 539,396 + HGI48_RS02495 0.69 -2.0
Or see this region's nucleotide sequence