Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS11125 and HGI48_RS11130 are separated by 13 nucleotides HGI48_RS11130 and HGI48_RS11135 are separated by 245 nucleotides HGI48_RS11135 and HGI48_RS11140 are separated by 37 nucleotides
HGI48_RS11125: HGI48_RS11125 - pectate lyase, at 2,421,401 to 2,423,218
_RS11125
HGI48_RS11130: HGI48_RS11130 - DNA-binding protein, at 2,423,232 to 2,423,564
_RS11130
HGI48_RS11135: HGI48_RS11135 - SDR family oxidoreductase, at 2,423,810 to 2,424,484
_RS11135
HGI48_RS11140: HGI48_RS11140 - type III effector HrpK, at 2,424,522 to 2,426,228
_RS11140
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2422.854 kb on + strand, within HGI48_RS11125 at 2422.854 kb on + strand, within HGI48_RS11125 at 2422.854 kb on + strand, within HGI48_RS11125 at 2422.854 kb on + strand, within HGI48_RS11125 at 2422.855 kb on - strand at 2422.855 kb on - strand at 2422.855 kb on - strand, within HGI48_RS11125 at 2422.855 kb on - strand, within HGI48_RS11125 at 2422.855 kb on - strand at 2422.855 kb on - strand, within HGI48_RS11125 at 2422.855 kb on - strand, within HGI48_RS11125 at 2422.855 kb on - strand, within HGI48_RS11125 at 2422.985 kb on - strand, within HGI48_RS11125 at 2423.098 kb on + strand at 2423.098 kb on + strand at 2423.098 kb on + strand at 2423.099 kb on - strand at 2423.146 kb on + strand at 2423.180 kb on + strand at 2423.180 kb on + strand at 2423.181 kb on - strand at 2423.192 kb on - strand at 2423.192 kb on - strand at 2423.211 kb on + strand at 2423.211 kb on + strand at 2423.211 kb on + strand at 2423.211 kb on + strand at 2423.211 kb on + strand at 2423.211 kb on + strand at 2423.212 kb on - strand at 2423.212 kb on - strand at 2423.212 kb on - strand at 2423.212 kb on - strand at 2423.212 kb on - strand at 2423.212 kb on - strand at 2423.216 kb on + strand at 2423.292 kb on + strand, within HGI48_RS11130 at 2423.293 kb on - strand, within HGI48_RS11130 at 2423.298 kb on - strand, within HGI48_RS11130 at 2423.562 kb on + strand at 2423.563 kb on - strand at 2423.563 kb on - strand at 2423.563 kb on - strand at 2423.563 kb on - strand at 2423.615 kb on + strand at 2423.617 kb on + strand at 2423.687 kb on - strand at 2423.707 kb on - strand at 2423.723 kb on + strand at 2423.726 kb on + strand at 2423.726 kb on + strand at 2423.761 kb on + strand at 2423.926 kb on + strand, within HGI48_RS11135 at 2423.926 kb on + strand, within HGI48_RS11135 at 2423.926 kb on + strand, within HGI48_RS11135 at 2423.926 kb on + strand, within HGI48_RS11135 at 2423.926 kb on + strand, within HGI48_RS11135 at 2423.927 kb on - strand, within HGI48_RS11135 at 2423.927 kb on - strand at 2423.927 kb on - strand at 2424.041 kb on - strand, within HGI48_RS11135 at 2424.245 kb on + strand, within HGI48_RS11135 at 2424.246 kb on - strand, within HGI48_RS11135 at 2424.246 kb on - strand, within HGI48_RS11135 at 2424.246 kb on - strand, within HGI48_RS11135 at 2424.330 kb on + strand, within HGI48_RS11135 at 2424.331 kb on - strand, within HGI48_RS11135 at 2424.331 kb on - strand at 2424.385 kb on - strand, within HGI48_RS11135 at 2424.653 kb on + strand at 2425.132 kb on + strand, within HGI48_RS11140 at 2425.208 kb on - strand, within HGI48_RS11140 at 2425.223 kb on + strand, within HGI48_RS11140 at 2425.223 kb on + strand, within HGI48_RS11140 at 2425.224 kb on - strand, within HGI48_RS11140 at 2425.224 kb on - strand, within HGI48_RS11140 at 2425.224 kb on - strand, within HGI48_RS11140 at 2425.227 kb on + strand, within HGI48_RS11140 at 2425.227 kb on + strand, within HGI48_RS11140 at 2425.227 kb on + strand, within HGI48_RS11140 at 2425.227 kb on + strand, within HGI48_RS11140 at 2425.228 kb on - strand, within HGI48_RS11140 at 2425.228 kb on - strand, within HGI48_RS11140 at 2425.427 kb on + strand, within HGI48_RS11140
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 2,422,854 + HGI48_RS11125 0.80 +0.5 2,422,854 + HGI48_RS11125 0.80 +1.4 2,422,854 + HGI48_RS11125 0.80 +0.1 2,422,854 + HGI48_RS11125 0.80 +0.0 2,422,855 - -1.3 2,422,855 - +0.7 2,422,855 - HGI48_RS11125 0.80 -2.0 2,422,855 - HGI48_RS11125 0.80 +0.3 2,422,855 - +0.3 2,422,855 - HGI48_RS11125 0.80 -0.4 2,422,855 - HGI48_RS11125 0.80 -0.9 2,422,855 - HGI48_RS11125 0.80 -1.5 2,422,985 - HGI48_RS11125 0.87 -1.4 2,423,098 + -0.5 2,423,098 + -1.1 2,423,098 + -1.1 2,423,099 - -0.2 2,423,146 + -1.1 2,423,180 + -0.7 2,423,180 + -1.0 2,423,181 - -0.5 2,423,192 - +0.4 2,423,192 - -1.7 2,423,211 + -0.1 2,423,211 + +0.8 2,423,211 + -0.7 2,423,211 + +0.4 2,423,211 + -0.3 2,423,211 + +0.0 2,423,212 - +0.6 2,423,212 - +0.1 2,423,212 - -0.0 2,423,212 - +0.3 2,423,212 - +0.5 2,423,212 - -0.5 2,423,216 + -0.7 2,423,292 + HGI48_RS11130 0.18 +0.9 2,423,293 - HGI48_RS11130 0.18 -0.7 2,423,298 - HGI48_RS11130 0.20 -0.5 2,423,562 + +0.0 2,423,563 - -0.3 2,423,563 - +1.2 2,423,563 - -2.3 2,423,563 - -1.1 2,423,615 + -0.4 2,423,617 + -1.7 2,423,687 - -0.9 2,423,707 - -0.5 2,423,723 + +0.3 2,423,726 + -0.2 2,423,726 + +0.3 2,423,761 + +0.6 2,423,926 + HGI48_RS11135 0.17 +2.1 2,423,926 + HGI48_RS11135 0.17 -0.5 2,423,926 + HGI48_RS11135 0.17 +0.3 2,423,926 + HGI48_RS11135 0.17 -2.7 2,423,926 + HGI48_RS11135 0.17 -1.0 2,423,927 - HGI48_RS11135 0.17 -1.3 2,423,927 - -0.3 2,423,927 - -1.3 2,424,041 - HGI48_RS11135 0.34 -0.6 2,424,245 + HGI48_RS11135 0.64 -0.3 2,424,246 - HGI48_RS11135 0.65 -3.0 2,424,246 - HGI48_RS11135 0.65 -0.9 2,424,246 - HGI48_RS11135 0.65 -0.1 2,424,330 + HGI48_RS11135 0.77 -0.2 2,424,331 - HGI48_RS11135 0.77 -2.3 2,424,331 - +1.3 2,424,385 - HGI48_RS11135 0.85 -0.7 2,424,653 + +0.3 2,425,132 + HGI48_RS11140 0.36 -2.0 2,425,208 - HGI48_RS11140 0.40 -1.2 2,425,223 + HGI48_RS11140 0.41 +0.0 2,425,223 + HGI48_RS11140 0.41 +0.0 2,425,224 - HGI48_RS11140 0.41 -1.3 2,425,224 - HGI48_RS11140 0.41 -0.3 2,425,224 - HGI48_RS11140 0.41 -0.7 2,425,227 + HGI48_RS11140 0.41 -0.7 2,425,227 + HGI48_RS11140 0.41 +0.9 2,425,227 + HGI48_RS11140 0.41 -0.4 2,425,227 + HGI48_RS11140 0.41 -0.9 2,425,228 - HGI48_RS11140 0.41 -2.0 2,425,228 - HGI48_RS11140 0.41 -0.7 2,425,427 + HGI48_RS11140 0.53 +0.3
Or see this region's nucleotide sequence