Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS06215 and HGI48_RS06220 are separated by 36 nucleotides HGI48_RS06220 and HGI48_RS06225 are separated by 1 nucleotides HGI48_RS06225 and HGI48_RS06230 are separated by 157 nucleotides HGI48_RS06230 and HGI48_RS06235 overlap by 11 nucleotides
HGI48_RS06215: HGI48_RS06215 - peptidoglycan DD-transpeptidase MrdA, at 1,320,347 to 1,322,257
_RS06215
HGI48_RS06220: HGI48_RS06220 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,322,294 to 1,322,764
_RS06220
HGI48_RS06225: HGI48_RS06225 - ribosome silencing factor, at 1,322,766 to 1,323,083
_RS06225
HGI48_RS06230: HGI48_RS06230 - nicotinate-nucleotide adenylyltransferase, at 1,323,241 to 1,323,915
_RS06230
HGI48_RS06235: HGI48_RS06235 - DNA polymerase III subunit delta, at 1,323,905 to 1,324,936
_RS06235
Position (kb)
1323
1324 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1322.243 kb on - strand at 1322.243 kb on - strand at 1322.250 kb on - strand at 1322.250 kb on - strand at 1322.296 kb on - strand at 1322.324 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.327 kb on - strand at 1322.367 kb on - strand, within HGI48_RS06220 at 1322.621 kb on - strand, within HGI48_RS06220 at 1322.827 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.970 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.976 kb on - strand, within HGI48_RS06225 at 1322.988 kb on - strand, within HGI48_RS06225 at 1323.003 kb on + strand, within HGI48_RS06225 at 1323.003 kb on + strand, within HGI48_RS06225 at 1323.004 kb on - strand, within HGI48_RS06225 at 1323.148 kb on - strand at 1323.203 kb on + strand at 1323.203 kb on + strand at 1323.205 kb on + strand at 1323.216 kb on + strand at 1323.216 kb on + strand at 1323.218 kb on + strand at 1323.218 kb on + strand at 1323.218 kb on + strand at 1323.218 kb on + strand at 1323.218 kb on + strand at 1323.218 kb on + strand at 1323.219 kb on - strand at 1323.229 kb on - strand at 1323.909 kb on - strand at 1323.909 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 1,322,243 - +1.0 1,322,243 - -1.2 1,322,250 - -2.2 1,322,250 - +1.4 1,322,296 - -1.2 1,322,324 - +0.4 1,322,327 - -0.4 1,322,327 - -1.0 1,322,327 - -1.2 1,322,327 - +1.2 1,322,327 - +0.0 1,322,327 - +1.0 1,322,327 - -2.2 1,322,367 - HGI48_RS06220 0.15 -1.0 1,322,621 - HGI48_RS06220 0.69 +0.6 1,322,827 - HGI48_RS06225 0.19 +0.8 1,322,970 - HGI48_RS06225 0.64 -0.1 1,322,970 - HGI48_RS06225 0.64 -0.1 1,322,970 - HGI48_RS06225 0.64 +0.9 1,322,970 - HGI48_RS06225 0.64 -0.2 1,322,970 - HGI48_RS06225 0.64 -1.7 1,322,970 - HGI48_RS06225 0.64 -0.1 1,322,970 - HGI48_RS06225 0.64 +0.4 1,322,970 - HGI48_RS06225 0.64 +0.9 1,322,970 - HGI48_RS06225 0.64 -0.8 1,322,970 - +1.0 1,322,970 - HGI48_RS06225 0.64 -1.6 1,322,970 - HGI48_RS06225 0.64 -0.1 1,322,976 - HGI48_RS06225 0.66 -1.0 1,322,976 - HGI48_RS06225 0.66 -0.4 1,322,976 - HGI48_RS06225 0.66 +0.1 1,322,976 - HGI48_RS06225 0.66 -0.2 1,322,976 - HGI48_RS06225 0.66 -3.2 1,322,976 - HGI48_RS06225 0.66 +1.1 1,322,976 - HGI48_RS06225 0.66 +0.5 1,322,976 - HGI48_RS06225 0.66 +0.3 1,322,976 - HGI48_RS06225 0.66 +0.4 1,322,976 - HGI48_RS06225 0.66 -1.2 1,322,976 - HGI48_RS06225 0.66 -0.1 1,322,976 - HGI48_RS06225 0.66 -1.4 1,322,988 - HGI48_RS06225 0.70 +0.7 1,323,003 + HGI48_RS06225 0.75 -2.2 1,323,003 + HGI48_RS06225 0.75 -1.0 1,323,004 - HGI48_RS06225 0.75 -0.2 1,323,148 - -0.2 1,323,203 + -1.2 1,323,203 + -2.7 1,323,205 + -1.2 1,323,216 + -1.4 1,323,216 + -2.8 1,323,218 + +1.4 1,323,218 + +3.3 1,323,218 + +0.0 1,323,218 + -2.0 1,323,218 + +0.2 1,323,218 + +0.6 1,323,219 - -2.2 1,323,229 - -1.2 1,323,909 - +0.2 1,323,909 - -0.2
Or see this region's nucleotide sequence