Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05025 and HGI48_RS05030 are separated by 22 nucleotides HGI48_RS05030 and HGI48_RS05035 are separated by 207 nucleotides HGI48_RS05035 and HGI48_RS05040 overlap by 13 nucleotides HGI48_RS05040 and HGI48_RS05045 are separated by 3 nucleotides
HGI48_RS05025: HGI48_RS05025 - D-2-hydroxyacid dehydrogenase family protein, at 1,054,284 to 1,055,297
_RS05025
HGI48_RS05030: HGI48_RS05030 - GNAT family N-acetyltransferase, at 1,055,320 to 1,055,766
_RS05030
HGI48_RS05035: HGI48_RS05035 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 1,055,974 to 1,056,807
_RS05035
HGI48_RS05040: HGI48_RS05040 - cysteine desulfurase sulfur acceptor subunit CsdE, at 1,056,795 to 1,057,238
_RS05040
HGI48_RS05045: HGI48_RS05045 - cysteine desulfurase CsdA, at 1,057,242 to 1,058,447
_RS05045
Position (kb)
1055
1056
1057 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1055.129 kb on + strand, within HGI48_RS05025 at 1055.159 kb on + strand, within HGI48_RS05025 at 1055.195 kb on + strand, within HGI48_RS05025 at 1055.197 kb on + strand at 1055.197 kb on + strand at 1055.197 kb on + strand at 1055.198 kb on - strand at 1055.198 kb on - strand at 1055.198 kb on - strand at 1055.205 kb on - strand at 1055.205 kb on - strand at 1055.283 kb on + strand at 1055.316 kb on + strand at 1055.316 kb on + strand at 1055.317 kb on - strand at 1055.317 kb on - strand at 1055.321 kb on + strand at 1055.322 kb on - strand at 1055.322 kb on - strand at 1055.397 kb on - strand at 1055.470 kb on + strand, within HGI48_RS05030 at 1055.486 kb on - strand, within HGI48_RS05030 at 1055.540 kb on + strand, within HGI48_RS05030 at 1055.540 kb on + strand, within HGI48_RS05030 at 1055.540 kb on + strand, within HGI48_RS05030 at 1055.541 kb on - strand, within HGI48_RS05030 at 1055.541 kb on - strand, within HGI48_RS05030 at 1055.571 kb on - strand at 1055.571 kb on - strand, within HGI48_RS05030 at 1055.658 kb on + strand, within HGI48_RS05030 at 1055.659 kb on - strand, within HGI48_RS05030 at 1055.659 kb on - strand, within HGI48_RS05030 at 1055.687 kb on + strand, within HGI48_RS05030 at 1055.687 kb on + strand, within HGI48_RS05030 at 1055.688 kb on - strand at 1055.688 kb on - strand, within HGI48_RS05030 at 1055.697 kb on + strand, within HGI48_RS05030 at 1055.698 kb on - strand, within HGI48_RS05030 at 1055.852 kb on - strand at 1055.855 kb on + strand at 1055.855 kb on + strand at 1055.855 kb on + strand at 1055.856 kb on - strand at 1055.870 kb on - strand at 1055.870 kb on - strand at 1055.870 kb on - strand at 1055.870 kb on - strand at 1055.870 kb on - strand at 1055.979 kb on + strand at 1055.979 kb on + strand at 1055.980 kb on - strand at 1055.998 kb on + strand at 1055.998 kb on + strand at 1055.999 kb on - strand at 1055.999 kb on - strand at 1056.002 kb on + strand at 1056.003 kb on - strand at 1056.031 kb on + strand at 1056.080 kb on + strand, within HGI48_RS05035 at 1056.343 kb on + strand, within HGI48_RS05035 at 1056.343 kb on + strand at 1056.343 kb on + strand, within HGI48_RS05035 at 1056.343 kb on + strand, within HGI48_RS05035 at 1056.344 kb on - strand, within HGI48_RS05035 at 1056.550 kb on - strand, within HGI48_RS05035 at 1056.607 kb on + strand, within HGI48_RS05035 at 1056.699 kb on + strand, within HGI48_RS05035 at 1056.699 kb on + strand, within HGI48_RS05035 at 1056.700 kb on - strand, within HGI48_RS05035 at 1056.700 kb on - strand, within HGI48_RS05035 at 1056.700 kb on - strand, within HGI48_RS05035 at 1056.700 kb on - strand, within HGI48_RS05035 at 1056.946 kb on - strand, within HGI48_RS05040 at 1057.218 kb on + strand at 1057.218 kb on + strand at 1057.218 kb on + strand at 1057.219 kb on - strand at 1057.219 kb on - strand at 1057.254 kb on + strand at 1057.254 kb on + strand at 1057.255 kb on - strand at 1057.310 kb on + strand at 1057.311 kb on - strand at 1057.312 kb on + strand at 1057.312 kb on + strand at 1057.312 kb on + strand at 1057.313 kb on - strand at 1057.313 kb on - strand at 1057.313 kb on - strand at 1057.440 kb on + strand, within HGI48_RS05045 at 1057.440 kb on + strand, within HGI48_RS05045 at 1057.440 kb on + strand, within HGI48_RS05045 at 1057.440 kb on + strand, within HGI48_RS05045 at 1057.440 kb on + strand, within HGI48_RS05045 at 1057.441 kb on - strand, within HGI48_RS05045 at 1057.454 kb on + strand, within HGI48_RS05045 at 1057.547 kb on + strand, within HGI48_RS05045 at 1057.548 kb on - strand, within HGI48_RS05045 at 1057.548 kb on - strand, within HGI48_RS05045 at 1057.668 kb on - strand, within HGI48_RS05045 at 1057.778 kb on - strand, within HGI48_RS05045 at 1057.778 kb on - strand, within HGI48_RS05045 at 1057.801 kb on + strand, within HGI48_RS05045 at 1057.801 kb on + strand, within HGI48_RS05045 at 1057.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 1,055,129 + HGI48_RS05025 0.83 +0.7 1,055,159 + HGI48_RS05025 0.86 +0.2 1,055,195 + HGI48_RS05025 0.90 +0.2 1,055,197 + -0.8 1,055,197 + -1.3 1,055,197 + -1.7 1,055,198 - -1.7 1,055,198 - -0.0 1,055,198 - +0.7 1,055,205 - -0.6 1,055,205 - -0.2 1,055,283 + -0.0 1,055,316 + +2.5 1,055,316 + -0.3 1,055,317 - -0.3 1,055,317 - +0.1 1,055,321 + -1.9 1,055,322 - +1.5 1,055,322 - +1.0 1,055,397 - +3.8 1,055,470 + HGI48_RS05030 0.34 -0.9 1,055,486 - HGI48_RS05030 0.37 +0.9 1,055,540 + HGI48_RS05030 0.49 -0.1 1,055,540 + HGI48_RS05030 0.49 +0.6 1,055,540 + HGI48_RS05030 0.49 +1.1 1,055,541 - HGI48_RS05030 0.49 +0.1 1,055,541 - HGI48_RS05030 0.49 -0.7 1,055,571 - +2.0 1,055,571 - HGI48_RS05030 0.56 -0.6 1,055,658 + HGI48_RS05030 0.76 -0.8 1,055,659 - HGI48_RS05030 0.76 +0.2 1,055,659 - HGI48_RS05030 0.76 +0.7 1,055,687 + HGI48_RS05030 0.82 -0.3 1,055,687 + HGI48_RS05030 0.82 -2.1 1,055,688 - +0.1 1,055,688 - HGI48_RS05030 0.82 -0.3 1,055,697 + HGI48_RS05030 0.84 +0.7 1,055,698 - HGI48_RS05030 0.85 -0.3 1,055,852 - +0.6 1,055,855 + +0.3 1,055,855 + +0.4 1,055,855 + -0.7 1,055,856 - -0.7 1,055,870 - -0.3 1,055,870 - +0.4 1,055,870 - -1.9 1,055,870 - -0.9 1,055,870 - -0.1 1,055,979 + -0.1 1,055,979 + -1.2 1,055,980 - -0.7 1,055,998 + -0.2 1,055,998 + -1.3 1,055,999 - -0.8 1,055,999 - -0.3 1,056,002 + +0.4 1,056,003 - -0.6 1,056,031 + -0.9 1,056,080 + HGI48_RS05035 0.13 -0.6 1,056,343 + HGI48_RS05035 0.44 -2.3 1,056,343 + +0.1 1,056,343 + HGI48_RS05035 0.44 -0.7 1,056,343 + HGI48_RS05035 0.44 -1.9 1,056,344 - HGI48_RS05035 0.44 -1.1 1,056,550 - HGI48_RS05035 0.69 -1.9 1,056,607 + HGI48_RS05035 0.76 -0.5 1,056,699 + HGI48_RS05035 0.87 -0.6 1,056,699 + HGI48_RS05035 0.87 -1.6 1,056,700 - HGI48_RS05035 0.87 +0.0 1,056,700 - HGI48_RS05035 0.87 +0.2 1,056,700 - HGI48_RS05035 0.87 -0.1 1,056,700 - HGI48_RS05035 0.87 -1.0 1,056,946 - HGI48_RS05040 0.34 -0.2 1,057,218 + -1.3 1,057,218 + -0.2 1,057,218 + -2.2 1,057,219 - -1.3 1,057,219 - -0.3 1,057,254 + +1.1 1,057,254 + -0.3 1,057,255 - -1.7 1,057,310 + +0.2 1,057,311 - -1.5 1,057,312 + +0.1 1,057,312 + -0.4 1,057,312 + +0.7 1,057,313 - -1.8 1,057,313 - +0.4 1,057,313 - +0.1 1,057,440 + HGI48_RS05045 0.16 +1.0 1,057,440 + HGI48_RS05045 0.16 +0.4 1,057,440 + HGI48_RS05045 0.16 -0.7 1,057,440 + HGI48_RS05045 0.16 -0.9 1,057,440 + HGI48_RS05045 0.16 -1.9 1,057,441 - HGI48_RS05045 0.17 -0.3 1,057,454 + HGI48_RS05045 0.18 -1.8 1,057,547 + HGI48_RS05045 0.25 -0.3 1,057,548 - HGI48_RS05045 0.25 -0.3 1,057,548 - HGI48_RS05045 0.25 -1.3 1,057,668 - HGI48_RS05045 0.35 -0.3 1,057,778 - HGI48_RS05045 0.44 -0.0 1,057,778 - HGI48_RS05045 0.44 -0.5 1,057,801 + HGI48_RS05045 0.46 +0.4 1,057,801 + HGI48_RS05045 0.46 -1.6 1,057,802 - -0.9
Or see this region's nucleotide sequence