Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS11720 and HGI48_RS11725 are separated by 3 nucleotides HGI48_RS11725 and HGI48_RS11730 are separated by 11 nucleotides HGI48_RS11730 and HGI48_RS11735 overlap by 1 nucleotides
HGI48_RS11720: HGI48_RS11720 - type III secretion system gatekeeper subunit SctW, at 2,560,327 to 2,561,496
_RS11720
HGI48_RS11725: HGI48_RS11725 - type III secretion system export apparatus subunit SctV, at 2,561,500 to 2,563,602
_RS11725
HGI48_RS11730: HGI48_RS11730 - type III secretion system inner membrane ring subunit SctD, at 2,563,614 to 2,564,576
_RS11730
HGI48_RS11735: HGI48_RS11735 - type III secretion system ATPase SctN, at 2,564,576 to 2,565,955
_RS11735
Position (kb)
2561
2562
2563
2564 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2560.930 kb on + strand, within HGI48_RS11720 at 2560.931 kb on - strand at 2560.931 kb on - strand, within HGI48_RS11720 at 2560.931 kb on - strand, within HGI48_RS11720 at 2561.137 kb on - strand, within HGI48_RS11720 at 2561.228 kb on + strand, within HGI48_RS11720 at 2561.228 kb on + strand, within HGI48_RS11720 at 2561.229 kb on - strand, within HGI48_RS11720 at 2561.252 kb on - strand, within HGI48_RS11720 at 2561.655 kb on + strand at 2561.656 kb on - strand at 2561.656 kb on - strand at 2561.686 kb on + strand at 2561.686 kb on + strand at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.205 kb on + strand, within HGI48_RS11725 at 2562.206 kb on - strand, within HGI48_RS11725 at 2562.206 kb on - strand, within HGI48_RS11725 at 2562.206 kb on - strand at 2562.206 kb on - strand, within HGI48_RS11725 at 2562.206 kb on - strand, within HGI48_RS11725 at 2562.324 kb on + strand, within HGI48_RS11725 at 2562.324 kb on + strand at 2562.324 kb on + strand, within HGI48_RS11725 at 2562.325 kb on - strand, within HGI48_RS11725 at 2562.325 kb on - strand at 2562.325 kb on - strand, within HGI48_RS11725 at 2562.441 kb on - strand, within HGI48_RS11725 at 2562.470 kb on + strand, within HGI48_RS11725 at 2562.679 kb on + strand, within HGI48_RS11725 at 2562.679 kb on + strand, within HGI48_RS11725 at 2562.679 kb on + strand, within HGI48_RS11725 at 2562.680 kb on - strand, within HGI48_RS11725 at 2562.694 kb on - strand, within HGI48_RS11725 at 2562.694 kb on - strand, within HGI48_RS11725 at 2563.005 kb on + strand, within HGI48_RS11725 at 2563.113 kb on - strand, within HGI48_RS11725 at 2563.222 kb on + strand, within HGI48_RS11725 at 2563.223 kb on - strand, within HGI48_RS11725 at 2563.261 kb on + strand, within HGI48_RS11725 at 2563.261 kb on + strand, within HGI48_RS11725 at 2563.262 kb on - strand, within HGI48_RS11725 at 2563.376 kb on - strand, within HGI48_RS11725 at 2563.440 kb on - strand at 2563.600 kb on + strand at 2563.600 kb on + strand at 2563.600 kb on + strand at 2563.600 kb on + strand at 2563.719 kb on + strand, within HGI48_RS11730 at 2563.719 kb on + strand, within HGI48_RS11730 at 2563.720 kb on - strand, within HGI48_RS11730 at 2564.083 kb on - strand, within HGI48_RS11730 at 2564.358 kb on + strand, within HGI48_RS11730 at 2564.359 kb on - strand, within HGI48_RS11730 at 2564.423 kb on - strand, within HGI48_RS11730
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 2,560,930 + HGI48_RS11720 0.52 +0.2 2,560,931 - +1.2 2,560,931 - HGI48_RS11720 0.52 +0.2 2,560,931 - HGI48_RS11720 0.52 -3.0 2,561,137 - HGI48_RS11720 0.69 -2.1 2,561,228 + HGI48_RS11720 0.77 -1.0 2,561,228 + HGI48_RS11720 0.77 +0.8 2,561,229 - HGI48_RS11720 0.77 -0.2 2,561,252 - HGI48_RS11720 0.79 -0.6 2,561,655 + -0.5 2,561,656 - -0.4 2,561,656 - -0.0 2,561,686 + +1.4 2,561,686 + -1.1 2,562,205 + HGI48_RS11725 0.34 +0.4 2,562,205 + HGI48_RS11725 0.34 +0.2 2,562,205 + HGI48_RS11725 0.34 -0.0 2,562,205 + HGI48_RS11725 0.34 +0.5 2,562,205 + HGI48_RS11725 0.34 -0.5 2,562,205 + HGI48_RS11725 0.34 -2.1 2,562,206 - HGI48_RS11725 0.34 -0.5 2,562,206 - HGI48_RS11725 0.34 +0.2 2,562,206 - -0.4 2,562,206 - HGI48_RS11725 0.34 -1.0 2,562,206 - HGI48_RS11725 0.34 +0.4 2,562,324 + HGI48_RS11725 0.39 -2.1 2,562,324 + +1.9 2,562,324 + HGI48_RS11725 0.39 +1.1 2,562,325 - HGI48_RS11725 0.39 +0.4 2,562,325 - -0.8 2,562,325 - HGI48_RS11725 0.39 -0.1 2,562,441 - HGI48_RS11725 0.45 -1.4 2,562,470 + HGI48_RS11725 0.46 -0.5 2,562,679 + HGI48_RS11725 0.56 +0.8 2,562,679 + HGI48_RS11725 0.56 +1.0 2,562,679 + HGI48_RS11725 0.56 +1.4 2,562,680 - HGI48_RS11725 0.56 -1.4 2,562,694 - HGI48_RS11725 0.57 -1.2 2,562,694 - HGI48_RS11725 0.57 +0.2 2,563,005 + HGI48_RS11725 0.72 -1.1 2,563,113 - HGI48_RS11725 0.77 -0.1 2,563,222 + HGI48_RS11725 0.82 +1.0 2,563,223 - HGI48_RS11725 0.82 +0.1 2,563,261 + HGI48_RS11725 0.84 -1.6 2,563,261 + HGI48_RS11725 0.84 +0.9 2,563,262 - HGI48_RS11725 0.84 +0.2 2,563,376 - HGI48_RS11725 0.89 +0.6 2,563,440 - -1.2 2,563,600 + +0.4 2,563,600 + +0.2 2,563,600 + -0.8 2,563,600 + -0.6 2,563,719 + HGI48_RS11730 0.11 +0.6 2,563,719 + HGI48_RS11730 0.11 -0.2 2,563,720 - HGI48_RS11730 0.11 -0.1 2,564,083 - HGI48_RS11730 0.49 +1.2 2,564,358 + HGI48_RS11730 0.77 -0.5 2,564,359 - HGI48_RS11730 0.77 +0.7 2,564,423 - HGI48_RS11730 0.84 -0.8
Or see this region's nucleotide sequence