Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS10020 and HGI48_RS10025 are separated by 96 nucleotides HGI48_RS10025 and HGI48_RS10030 are separated by 78 nucleotides
HGI48_RS10020: HGI48_RS10020 - murein biosynthesis integral membrane protein MurJ, at 2,163,632 to 2,165,167
_RS10020
HGI48_RS10025: HGI48_RS10025 - LysR family transcriptional regulator, at 2,165,264 to 2,166,175
_RS10025
HGI48_RS10030: HGI48_RS10030 - NAD(P)-dependent alcohol dehydrogenase, at 2,166,254 to 2,167,270
_RS10030
Position (kb)
2165
2166
2167 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2165.168 kb on + strand at 2165.266 kb on - strand at 2165.267 kb on + strand at 2165.267 kb on + strand at 2165.267 kb on + strand at 2165.267 kb on + strand at 2165.267 kb on + strand at 2165.268 kb on - strand at 2165.268 kb on - strand at 2165.272 kb on + strand at 2165.272 kb on + strand at 2165.272 kb on + strand at 2165.272 kb on + strand at 2165.286 kb on + strand at 2165.287 kb on - strand at 2165.334 kb on + strand at 2165.338 kb on - strand at 2165.338 kb on - strand at 2165.338 kb on - strand at 2165.338 kb on - strand at 2165.338 kb on - strand at 2165.338 kb on - strand at 2165.362 kb on + strand, within HGI48_RS10025 at 2165.404 kb on + strand at 2165.404 kb on + strand at 2165.404 kb on + strand, within HGI48_RS10025 at 2165.405 kb on - strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.504 kb on + strand, within HGI48_RS10025 at 2165.555 kb on + strand, within HGI48_RS10025 at 2165.556 kb on - strand, within HGI48_RS10025 at 2165.753 kb on - strand at 2165.837 kb on + strand at 2165.838 kb on - strand at 2165.874 kb on - strand, within HGI48_RS10025 at 2165.912 kb on + strand, within HGI48_RS10025 at 2165.913 kb on - strand, within HGI48_RS10025 at 2165.954 kb on + strand, within HGI48_RS10025 at 2165.954 kb on + strand, within HGI48_RS10025 at 2165.998 kb on + strand, within HGI48_RS10025 at 2165.998 kb on + strand, within HGI48_RS10025 at 2165.998 kb on + strand, within HGI48_RS10025 at 2165.999 kb on - strand, within HGI48_RS10025 at 2165.999 kb on - strand, within HGI48_RS10025 at 2165.999 kb on - strand, within HGI48_RS10025 at 2166.070 kb on + strand, within HGI48_RS10025 at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.220 kb on + strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.221 kb on - strand at 2166.240 kb on - strand at 2166.517 kb on - strand, within HGI48_RS10030 at 2166.517 kb on - strand, within HGI48_RS10030 at 2166.578 kb on + strand, within HGI48_RS10030 at 2166.579 kb on - strand, within HGI48_RS10030 at 2166.579 kb on - strand, within HGI48_RS10030 at 2166.588 kb on - strand, within HGI48_RS10030 at 2166.588 kb on - strand at 2166.588 kb on - strand, within HGI48_RS10030 at 2166.662 kb on - strand, within HGI48_RS10030 at 2166.774 kb on + strand, within HGI48_RS10030 at 2166.890 kb on + strand, within HGI48_RS10030 at 2166.891 kb on - strand, within HGI48_RS10030 at 2167.024 kb on - strand, within HGI48_RS10030 at 2167.024 kb on - strand, within HGI48_RS10030 at 2167.075 kb on + strand, within HGI48_RS10030 at 2167.075 kb on + strand, within HGI48_RS10030 at 2167.075 kb on + strand, within HGI48_RS10030 at 2167.076 kb on - strand, within HGI48_RS10030 at 2167.076 kb on - strand, within HGI48_RS10030 at 2167.165 kb on + strand, within HGI48_RS10030
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 2,165,168 + +0.1 2,165,266 - +0.7 2,165,267 + +0.2 2,165,267 + -0.8 2,165,267 + +0.2 2,165,267 + -0.8 2,165,267 + +0.1 2,165,268 - -1.1 2,165,268 - +0.7 2,165,272 + -1.3 2,165,272 + -0.4 2,165,272 + -0.5 2,165,272 + +0.7 2,165,286 + -0.9 2,165,287 - +0.2 2,165,334 + +0.2 2,165,338 - +0.0 2,165,338 - -0.3 2,165,338 - -0.0 2,165,338 - +0.2 2,165,338 - -1.1 2,165,338 - -0.1 2,165,362 + HGI48_RS10025 0.11 -0.9 2,165,404 + +0.7 2,165,404 + +0.7 2,165,404 + HGI48_RS10025 0.15 -0.9 2,165,405 - HGI48_RS10025 0.15 -0.1 2,165,504 + HGI48_RS10025 0.26 -1.0 2,165,504 + HGI48_RS10025 0.26 -0.8 2,165,504 + HGI48_RS10025 0.26 +0.2 2,165,504 + HGI48_RS10025 0.26 +0.4 2,165,504 + HGI48_RS10025 0.26 +0.5 2,165,504 + HGI48_RS10025 0.26 +0.2 2,165,555 + HGI48_RS10025 0.32 -0.5 2,165,556 - HGI48_RS10025 0.32 +1.1 2,165,753 - -0.3 2,165,837 + -1.3 2,165,838 - +1.8 2,165,874 - HGI48_RS10025 0.67 +0.2 2,165,912 + HGI48_RS10025 0.71 -0.1 2,165,913 - HGI48_RS10025 0.71 +0.1 2,165,954 + HGI48_RS10025 0.76 +0.6 2,165,954 + HGI48_RS10025 0.76 +0.4 2,165,998 + HGI48_RS10025 0.80 -0.3 2,165,998 + HGI48_RS10025 0.80 -0.1 2,165,998 + HGI48_RS10025 0.80 -0.3 2,165,999 - HGI48_RS10025 0.81 -0.2 2,165,999 - HGI48_RS10025 0.81 -1.0 2,165,999 - HGI48_RS10025 0.81 -1.1 2,166,070 + HGI48_RS10025 0.88 -0.6 2,166,220 + -0.8 2,166,220 + -0.1 2,166,220 + -1.6 2,166,220 + +0.5 2,166,220 + +0.1 2,166,220 + -0.3 2,166,220 + -0.3 2,166,220 + +0.2 2,166,220 + +1.8 2,166,220 + -0.8 2,166,220 + +0.7 2,166,220 + +0.2 2,166,220 + -0.6 2,166,220 + +0.1 2,166,220 + +0.2 2,166,221 - +0.2 2,166,221 - +0.7 2,166,221 - +0.7 2,166,221 - +2.1 2,166,221 - +0.5 2,166,221 - +2.2 2,166,221 - +0.4 2,166,221 - +0.9 2,166,221 - -1.8 2,166,221 - -0.0 2,166,221 - -1.3 2,166,221 - -2.1 2,166,221 - -0.1 2,166,221 - -0.8 2,166,221 - +0.2 2,166,240 - +1.1 2,166,517 - HGI48_RS10030 0.26 -1.8 2,166,517 - HGI48_RS10030 0.26 +0.1 2,166,578 + HGI48_RS10030 0.32 -0.3 2,166,579 - HGI48_RS10030 0.32 +0.2 2,166,579 - HGI48_RS10030 0.32 +0.7 2,166,588 - HGI48_RS10030 0.33 -1.1 2,166,588 - +0.2 2,166,588 - HGI48_RS10030 0.33 -0.8 2,166,662 - HGI48_RS10030 0.40 +0.6 2,166,774 + HGI48_RS10030 0.51 -0.6 2,166,890 + HGI48_RS10030 0.63 +0.9 2,166,891 - HGI48_RS10030 0.63 -1.1 2,167,024 - HGI48_RS10030 0.76 -1.2 2,167,024 - HGI48_RS10030 0.76 +0.1 2,167,075 + HGI48_RS10030 0.81 -0.8 2,167,075 + HGI48_RS10030 0.81 -0.2 2,167,075 + HGI48_RS10030 0.81 -2.1 2,167,076 - HGI48_RS10030 0.81 -0.5 2,167,076 - HGI48_RS10030 0.81 +0.2 2,167,165 + HGI48_RS10030 0.90 -0.9
Or see this region's nucleotide sequence