Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS06525 and HGI48_RS06530 are separated by 16 nucleotides HGI48_RS06530 and HGI48_RS06535 overlap by 4 nucleotides HGI48_RS06535 and HGI48_RS06540 are separated by 134 nucleotides HGI48_RS06540 and HGI48_RS06545 overlap by 4 nucleotides HGI48_RS06545 and HGI48_RS06550 are separated by 12 nucleotides HGI48_RS06550 and HGI48_RS06555 are separated by 94 nucleotides
HGI48_RS06525: HGI48_RS06525 - cytochrome d ubiquinol oxidase subunit II, at 1,386,474 to 1,387,613
_RS06525
HGI48_RS06530: HGI48_RS06530 - cytochrome bd-I oxidase subunit CydX, at 1,387,630 to 1,387,746
_RS06530
HGI48_RS06535: HGI48_RS06535 - cyd operon protein YbgE, at 1,387,743 to 1,388,036
_RS06535
HGI48_RS06540: HGI48_RS06540 - tol-pal system-associated acyl-CoA thioesterase, at 1,388,171 to 1,388,575
_RS06540
HGI48_RS06545: HGI48_RS06545 - Tol-Pal system protein TolQ, at 1,388,572 to 1,389,258
_RS06545
HGI48_RS06550: HGI48_RS06550 - colicin uptake protein TolR, at 1,389,271 to 1,389,696
_RS06550
HGI48_RS06555: HGI48_RS06555 - cell envelope integrity protein TolA, at 1,389,791 to 1,390,975
_RS06555
Position (kb)
1388
1389
1390 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1387.615 kb on + strand at 1387.754 kb on + strand at 1387.776 kb on - strand, within HGI48_RS06535 at 1387.838 kb on - strand, within HGI48_RS06535 at 1387.838 kb on - strand, within HGI48_RS06535 at 1387.838 kb on - strand, within HGI48_RS06535 at 1387.959 kb on - strand, within HGI48_RS06535 at 1387.959 kb on - strand, within HGI48_RS06535 at 1388.019 kb on + strand at 1388.019 kb on + strand at 1388.019 kb on + strand at 1388.020 kb on - strand at 1388.020 kb on - strand at 1388.020 kb on - strand at 1388.022 kb on + strand at 1388.023 kb on - strand at 1388.025 kb on + strand at 1388.025 kb on + strand at 1388.025 kb on + strand at 1388.026 kb on - strand at 1388.026 kb on - strand at 1388.026 kb on - strand at 1388.081 kb on - strand at 1388.084 kb on + strand at 1388.085 kb on - strand at 1388.085 kb on - strand at 1388.085 kb on - strand at 1388.085 kb on - strand at 1388.098 kb on + strand at 1388.098 kb on + strand at 1388.099 kb on - strand at 1388.099 kb on - strand at 1388.102 kb on + strand at 1388.102 kb on + strand at 1388.103 kb on - strand at 1388.103 kb on - strand at 1388.103 kb on - strand at 1388.121 kb on + strand at 1388.121 kb on + strand at 1388.134 kb on + strand at 1388.135 kb on - strand at 1388.135 kb on - strand at 1388.136 kb on + strand at 1388.136 kb on + strand at 1388.136 kb on + strand at 1388.137 kb on - strand at 1388.137 kb on - strand at 1388.167 kb on + strand at 1388.167 kb on + strand at 1388.210 kb on + strand at 1388.210 kb on + strand at 1388.211 kb on - strand at 1388.218 kb on + strand, within HGI48_RS06540 at 1388.218 kb on + strand, within HGI48_RS06540 at 1388.218 kb on + strand, within HGI48_RS06540 at 1388.219 kb on - strand, within HGI48_RS06540 at 1388.219 kb on - strand at 1388.233 kb on + strand, within HGI48_RS06540 at 1388.233 kb on + strand, within HGI48_RS06540 at 1388.252 kb on + strand, within HGI48_RS06540 at 1388.252 kb on + strand, within HGI48_RS06540 at 1388.264 kb on + strand, within HGI48_RS06540 at 1388.264 kb on + strand, within HGI48_RS06540 at 1388.366 kb on + strand, within HGI48_RS06540 at 1388.367 kb on - strand, within HGI48_RS06540 at 1388.395 kb on - strand, within HGI48_RS06540 at 1388.421 kb on + strand, within HGI48_RS06540 at 1388.481 kb on + strand, within HGI48_RS06540 at 1388.482 kb on - strand, within HGI48_RS06540 at 1388.545 kb on + strand at 1388.546 kb on - strand at 1388.551 kb on + strand at 1388.551 kb on + strand at 1388.552 kb on - strand at 1388.586 kb on + strand at 1388.671 kb on - strand, within HGI48_RS06545 at 1388.767 kb on + strand, within HGI48_RS06545 at 1388.767 kb on + strand, within HGI48_RS06545 at 1388.821 kb on + strand at 1388.986 kb on + strand at 1388.986 kb on + strand at 1388.987 kb on - strand at 1388.987 kb on - strand, within HGI48_RS06545 at 1389.018 kb on + strand at 1389.145 kb on + strand, within HGI48_RS06545 at 1389.216 kb on + strand at 1389.270 kb on + strand at 1389.315 kb on + strand, within HGI48_RS06550 at 1389.318 kb on + strand, within HGI48_RS06550 at 1389.318 kb on + strand, within HGI48_RS06550 at 1389.319 kb on - strand, within HGI48_RS06550 at 1389.384 kb on + strand, within HGI48_RS06550 at 1389.487 kb on + strand, within HGI48_RS06550 at 1389.513 kb on + strand at 1389.616 kb on + strand, within HGI48_RS06550 at 1389.741 kb on + strand at 1389.742 kb on - strand at 1389.752 kb on + strand at 1389.753 kb on - strand at 1389.822 kb on + strand at 1389.822 kb on + strand at 1389.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 1,387,615 + +0.3 1,387,754 + -0.3 1,387,776 - HGI48_RS06535 0.11 -0.5 1,387,838 - HGI48_RS06535 0.32 +0.1 1,387,838 - HGI48_RS06535 0.32 +0.1 1,387,838 - HGI48_RS06535 0.32 +0.1 1,387,959 - HGI48_RS06535 0.73 +0.6 1,387,959 - HGI48_RS06535 0.73 -0.5 1,388,019 + -0.2 1,388,019 + +1.0 1,388,019 + +0.7 1,388,020 - +1.2 1,388,020 - -0.2 1,388,020 - +0.2 1,388,022 + -1.1 1,388,023 - +1.2 1,388,025 + -0.8 1,388,025 + +0.5 1,388,025 + -0.2 1,388,026 - -0.1 1,388,026 - -1.2 1,388,026 - +0.5 1,388,081 - +0.6 1,388,084 + +0.1 1,388,085 - -0.9 1,388,085 - +0.3 1,388,085 - -0.3 1,388,085 - -0.1 1,388,098 + +1.8 1,388,098 + +0.8 1,388,099 - -2.3 1,388,099 - -0.0 1,388,102 + -2.1 1,388,102 + -1.7 1,388,103 - -1.1 1,388,103 - -1.2 1,388,103 - -0.8 1,388,121 + -0.2 1,388,121 + -0.1 1,388,134 + +0.7 1,388,135 - -1.8 1,388,135 - -1.1 1,388,136 + -1.3 1,388,136 + +0.9 1,388,136 + +0.2 1,388,137 - -1.1 1,388,137 - -1.5 1,388,167 + -0.7 1,388,167 + -1.1 1,388,210 + -1.8 1,388,210 + +0.1 1,388,211 - +0.5 1,388,218 + HGI48_RS06540 0.12 -0.7 1,388,218 + HGI48_RS06540 0.12 +0.2 1,388,218 + HGI48_RS06540 0.12 -1.5 1,388,219 - HGI48_RS06540 0.12 +0.6 1,388,219 - +2.1 1,388,233 + HGI48_RS06540 0.15 -0.9 1,388,233 + HGI48_RS06540 0.15 -1.2 1,388,252 + HGI48_RS06540 0.20 -0.5 1,388,252 + HGI48_RS06540 0.20 -0.4 1,388,264 + HGI48_RS06540 0.23 -0.6 1,388,264 + HGI48_RS06540 0.23 +0.3 1,388,366 + HGI48_RS06540 0.48 -0.3 1,388,367 - HGI48_RS06540 0.48 +0.1 1,388,395 - HGI48_RS06540 0.55 +0.5 1,388,421 + HGI48_RS06540 0.62 -0.4 1,388,481 + HGI48_RS06540 0.77 +1.5 1,388,482 - HGI48_RS06540 0.77 +0.0 1,388,545 + -0.4 1,388,546 - +0.9 1,388,551 + +1.2 1,388,551 + +0.2 1,388,552 - -2.1 1,388,586 + -2.7 1,388,671 - HGI48_RS06545 0.14 -2.2 1,388,767 + HGI48_RS06545 0.28 -2.5 1,388,767 + HGI48_RS06545 0.28 -0.3 1,388,821 + -1.1 1,388,986 + -0.5 1,388,986 + -1.1 1,388,987 - -1.1 1,388,987 - HGI48_RS06545 0.60 -0.0 1,389,018 + -1.1 1,389,145 + HGI48_RS06545 0.83 -4.2 1,389,216 + +0.4 1,389,270 + -1.5 1,389,315 + HGI48_RS06550 0.10 +0.1 1,389,318 + HGI48_RS06550 0.11 -0.2 1,389,318 + HGI48_RS06550 0.11 -1.3 1,389,319 - HGI48_RS06550 0.11 +0.9 1,389,384 + HGI48_RS06550 0.27 +0.9 1,389,487 + HGI48_RS06550 0.51 -0.8 1,389,513 + -1.1 1,389,616 + HGI48_RS06550 0.81 +0.2 1,389,741 + -0.5 1,389,742 - -1.5 1,389,752 + -0.1 1,389,753 - -1.8 1,389,822 + -3.5 1,389,822 + -0.1 1,389,823 - +1.1
Or see this region's nucleotide sequence