Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00675 and HGI48_RS00680 are separated by 111 nucleotides HGI48_RS00680 and HGI48_RS00685 are separated by 65 nucleotides HGI48_RS00685 and HGI48_RS00690 are separated by 108 nucleotides HGI48_RS00690 and HGI48_RS00695 overlap by 32 nucleotides
HGI48_RS00675: HGI48_RS00675 - ribonuclease PH, at 158,964 to 159,680
_RS00675
HGI48_RS00680: HGI48_RS00680 - orotate phosphoribosyltransferase, at 159,792 to 160,433
_RS00680
HGI48_RS00685: HGI48_RS00685 - nucleoid occlusion factor SlmA, at 160,499 to 161,095
_RS00685
HGI48_RS00690: HGI48_RS00690 - dUTP diphosphatase, at 161,204 to 161,662
_RS00690
HGI48_RS00695: HGI48_RS00695 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 161,631 to 162,887
_RS00695
Position (kb) 160
161
162 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 159.713 kb on + strand at 159.714 kb on - strand at 159.714 kb on - strand at 159.714 kb on - strand at 159.714 kb on - strand at 159.716 kb on - strand at 159.716 kb on - strand at 159.729 kb on + strand at 159.729 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.734 kb on + strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.735 kb on - strand at 159.801 kb on + strand at 159.802 kb on - strand at 159.878 kb on + strand at 159.878 kb on + strand, within HGI48_RS00680 at 159.878 kb on + strand, within HGI48_RS00680 at 159.888 kb on + strand, within HGI48_RS00680 at 160.005 kb on + strand, within HGI48_RS00680 at 160.006 kb on - strand, within HGI48_RS00680 at 160.006 kb on - strand, within HGI48_RS00680 at 160.006 kb on - strand, within HGI48_RS00680 at 160.088 kb on + strand, within HGI48_RS00680 at 160.312 kb on - strand, within HGI48_RS00680 at 160.432 kb on - strand at 160.467 kb on + strand at 160.467 kb on + strand at 160.468 kb on - strand at 160.468 kb on - strand at 160.468 kb on - strand at 160.468 kb on - strand at 160.864 kb on + strand, within HGI48_RS00685 at 160.864 kb on + strand, within HGI48_RS00685 at 160.865 kb on - strand, within HGI48_RS00685 at 160.865 kb on - strand, within HGI48_RS00685 at 160.865 kb on - strand, within HGI48_RS00685 at 160.901 kb on - strand, within HGI48_RS00685 at 161.000 kb on - strand, within HGI48_RS00685 at 161.115 kb on - strand at 161.115 kb on - strand at 161.115 kb on - strand at 161.171 kb on + strand at 161.172 kb on - strand at 161.173 kb on + strand at 161.203 kb on - strand at 161.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 159,713 + +2.7 159,714 - -0.6 159,714 - -2.5 159,714 - -0.7 159,714 - +0.1 159,716 - +0.4 159,716 - -1.5 159,729 + -0.7 159,729 + +1.9 159,734 + -0.4 159,734 + +0.9 159,734 + +0.9 159,734 + -3.0 159,734 + +1.0 159,734 + -0.1 159,734 + -2.8 159,734 + +1.2 159,734 + -0.4 159,734 + -1.5 159,734 + -0.7 159,734 + -1.5 159,734 + +0.2 159,735 - -1.8 159,735 - +0.0 159,735 - -2.3 159,735 - -0.7 159,735 - -0.8 159,735 - +0.6 159,735 - -1.1 159,735 - -1.5 159,735 - -1.7 159,735 - +0.4 159,735 - -2.3 159,735 - -2.1 159,735 - -1.7 159,735 - -0.7 159,735 - -1.5 159,801 + -1.7 159,802 - -0.8 159,878 + +0.9 159,878 + HGI48_RS00680 0.13 -1.5 159,878 + HGI48_RS00680 0.13 -0.1 159,888 + HGI48_RS00680 0.15 -2.3 160,005 + HGI48_RS00680 0.33 -1.1 160,006 - HGI48_RS00680 0.33 -2.5 160,006 - HGI48_RS00680 0.33 -1.1 160,006 - HGI48_RS00680 0.33 -2.5 160,088 + HGI48_RS00680 0.46 +0.6 160,312 - HGI48_RS00680 0.81 -3.2 160,432 - -1.6 160,467 + -1.7 160,467 + -0.7 160,468 - +0.3 160,468 - +1.4 160,468 - +0.2 160,468 - +0.2 160,864 + HGI48_RS00685 0.61 -0.0 160,864 + HGI48_RS00685 0.61 +0.5 160,865 - HGI48_RS00685 0.61 -1.9 160,865 - HGI48_RS00685 0.61 -0.7 160,865 - HGI48_RS00685 0.61 -1.0 160,901 - HGI48_RS00685 0.67 -0.6 161,000 - HGI48_RS00685 0.84 -0.6 161,115 - -0.4 161,115 - -0.4 161,115 - -0.7 161,171 + -0.2 161,172 - -0.1 161,173 + +0.7 161,203 - -0.1 161,203 - +1.1
Or see this region's nucleotide sequence